Method for regulating and controlling bioactivity of lysozyme based on induced crystallization effect of ionic liquid

Abstract

The invention discloses a method for regulating and controlling bioactivity of lysozyme based on the induced crystallization effect of ionic liquid. The method comprises the following steps: (1) adding water-soluble imidazolium ionic liquid into a solution A which is a sodium acetate buffer solution of NaCl with a pH value of 4 to 6.5 and mass concentration of 3 to 6%; (2) adding egg white lysozyme into liquid obtained in the step (1), wherein a supersaturation ratio of the lysozyme is 1 to 3 at a temperature of 20 to 30 DEG C; and (3) cooling the liquid obtained in the step (2) with stirring until a crystal is precipitated, carrying out crystal growing in turbidity, then carrying out cooling so as to allow a crystallization solution to be obtained, filtering the crystallization solution, drying the crystal so as to obtain a lysozyme crystal product and preserving the lysozyme crystal product at a temperature of 4 DEG C. The method provided by the invention has the advantages of easy realization of process control and convenience in operation. The prepared lysozyme crystal product has uniform granularity, a small variable coefficient, main granularity of more than 100 mu m, mass yield of more than 90%, intact appearance and substantially improved relative activity.

Description

technical field [0001] The invention relates to a method for regulating the biological activity of lysozyme based on the induced crystallization effect of ionic liquid, which is suitable for the production field of lysozyme in the fields of medicine, chemical industry and food. Background technique [0002] Lysozyme is an important biomacromolecule with various pharmacological effects. With the development of biological science, lysozyme has become an indispensable tool enzyme in genetic engineering and enzyme engineering, which is used to manufacture and extract the active enzymes in bacteria. Substances such as nucleic acids, enzymes and active polypeptides. At the same time, it is a natural fungicide and preservative with good safety performance, which can be widely used in food preservation, pharmaceutical preparations, daily chemical industries and other industries. During the production and purification of lysozyme, due to changes in process conditions or the environm...

AI Technical Summary

Problems solved by technology

The research and application of lysozyme started relatively late in my country, and foreign countries have blocked the production and preparation technology of high-purity and high-activity lysozyme. Compared with developed countries, the domestic production and production technology of lysozyme is relatively backward, especially The production of high-activity and high-purity lysozyme is far from meeting the growing market demand in my country

Although some domestic researchers have carried out some basic research work on the extraction and application of lysozyme, for example, they have made simple improvements to the crystallization process of lysozyme. The activity has increased, but its quality still cannot compete with foreign products

[0004] Ionic liquids have the characteristics of low vapor pressure, low melting point, wide liquid range, easy operation, good solubility, high stability and designability. They have become important reaction media in electrochemistry, catalysis and organic synthesis, and are widely used in chemical separation. It has also attracted widespread attention, and there is no industrial method to induce the crystallization of lysozyme and regulate the biological activity of lysozyme by adding ionic liquids.

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