Compositions comprising peroxy alpha-ketocarboxylic acid and methods for producing and using the same
A composition and technology of ketone carboxylic acid, applied in the direction of active ingredients of peroxygen compounds, botany equipment and methods, medical preparations containing active ingredients, etc., can solve the problems of inability to kill spores, no effective treatment, etc.
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Embodiment 1
[0105] Disinfection of spores on medical devices
[0106] This example demonstrates the sporicidal efficacy of PKCA compounds in a dry, high protein environment using the method described in the ASTM E-2197 procedure. figure 1 The sporicidal activity of peroxy alpha-ketopyruvate (PPA), peroxy alpha-ketovalerate (POKVA) and peroxy alpha-ketobutyrate (POKBA) is illustrated. Each of these solutions challenged a 6 log kill of C. difficile spores in 10 minutes in a high protein environment. The required concentration is 1000 ppm (8.5 mM) for PPA and POKVA and 500 ppm (4.2 mM) for POKBA. Additionally, PPA and POKVA at a concentration of 750 mm (6.3 mM) and POKBA at a concentration of 250 ppm (2.1 mM) killed 3 logs of C. difficile. These concentrations of PKCA corresponded to alpha-ketoacid concentrations of 12.4 mM (1000 ppm), 9.3 mM (750 ppm) and 3.1 mM (250 ppm).
Embodiment 2
[0108] Disinfection of Biofilms on Medical Devices
[0109] The efficacy of PKCA against surface biofilm formation was tested by AO AC966.04 procedure. This protocol tests candidate disinfectants against Bacillus subtilis spores dried onto small ceramic penicylinders on which they can form biofilms. Briefly, prepare a final concentration equal to 1 to 4×10 7 CFU / mL of the dilution of the spore suspension in sterile distilled water. Using a sterile loop, place the sterile small cylinder in the prepared dilution and mix well, then incubate it for 10 to 15 minutes. Subsequently, the mobile cylinder was placed on a sterilized screen in a sterilized petri dish and then placed in a desiccator for at least 12 to 24 hours or until use. For the sanitizer test, small contaminated cylinders and uncontaminated control cylinders were placed in vials containing the PKCA mixture and allowed to solidify for 10 minutes. Subsequently, appropriate dilutions (usually a 1:1000 dilution of Spi...
Embodiment 3
[0111] Materials and methods
[0112] Strains and growth conditions
[0113] Pseudomonas aeruginosa PAO1 (ATCC number: BAA-47), Enterococcus faecalis V583 (ATCC number: 700802) and Staphylococcus aureus (ATCC number: 700699) were incubated at 37 °C in tryptic soybean meat Soup (tryptic soy broth) (TSB, Sigma Chemical Co., St. Louis, MO, USA) culture medium, while stirring for 16 hours.
[0114] Chemical treatments for biofilm formation
[0115]Multispecies biofilm formation was performed using Bolton broth (Oxoid Ltd, Basingstock, Hampshire, England) and bovine plasma (Biomeda, Foster City, CA, USA). Pseudomonas aeruginosa PAO1, Enterococcus faecalis V583 and Staphylococcus aureus cultured on TSB agar plates were inoculated into TSB broth and incubated at 37°C in a shaker for 16 hours. Aliquots of each individual bacterial type were diluted into serial dilutions in TSB broth. Diluted bacteria were plated to enumerate colony forming units (CFU). Dilute them further to 1×...
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