Natural product preparation for promoting rumen fermentation

A technology of natural products and preparations, applied in the field of rumen regulation, can solve problems such as shortage of feed resources and inability to meet the needs of the development of herbivorous livestock breeding

Inactive Publication Date: 2013-11-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, my country's feed grains are still far from meeting the increasing demand for livestock products. At the same time, the output of forage grass, which is the main source of feed for herbivorous livestock, has been una

Method used

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  • Natural product preparation for promoting rumen fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Use a traditional Chinese medicine pulverizer to pulverize the millennium roots into powder, weigh 0.25g into a 15mL centrifuge tube, and add 5mL of 70% ethanol solution;

[0022] (2) Vortex vibration→35rpm rotary mixer mixing for 30min→vortex vibration→rotary mixer mixing for 30min (two times total 1h)→3000r centrifugation for 5min;

[0023] (3) Suck the supernatant into a freezing bottle, and blow dry with a nitrogen blowing instrument;

[0024] (4) The precipitate is retained in the centrifuge tube, continue to add 5mL 70% ethanol → vortex shaker → rotary mixer for 30 min → vortex shaker → rotary mixer for 30 min → vortex shaker → rotary mixer for 30 min (total 1.5h for three times) → 3000r Centrifuge for 5 min;

[0025] (5) Pipette the supernatant into the blow-dried frozen bottle, blow it dry with a nitrogen blower, and freeze the dryer for 24-48h, wait until it rises to room temperature to weigh, seal the bottle, and store at -20°C.

Embodiment 2

[0027] (1) Use a traditional Chinese medicine pulverizer to pulverize the millennium roots into powder, weigh 0.25g into a 15mL centrifuge tube, and add 5mL 60% ethanol solution;

[0028] (2) Vortex vibration→35rpm rotary mixer mixing for 30min→vortex vibration→rotary mixer mixing for 30min (two times total 1h)→3000r centrifugation for 5min;

[0029] (3) Suck the supernatant into a freezing bottle, and blow dry with a nitrogen blowing instrument;

[0030] (4) The precipitate in the centrifuge tube is retained, continue to add 5mL 60% ethanol → vortex oscillator → rotary mixer for 30 min → vortex oscillator → rotary mixer for 30 min → vortex oscillator → rotary mixer for 30 min (total 1.5h for three times) → 3000r Centrifuge for 5 min;

[0031] (5) Pipette the supernatant into the blow-dried frozen bottle, blow it dry with a nitrogen blower, and freeze the dryer for 24-48h, wait until it rises to room temperature to weigh, seal the bottle, and store at -20°C.

Embodiment 3

[0033] (1) Use a traditional Chinese medicine pulverizer to pulverize the millennium roots into powder, weigh 0.25g into a 15mL centrifuge tube, and add 5mL of 80% ethanol solution;

[0034] (2) Vortex vibration→35rpm rotary mixer mixing for 30min→vortex vibration→rotary mixer mixing for 30min (two times total 1h)→3000r centrifugation for 5min;

[0035] (3) Suck the supernatant into a freezing bottle, and blow dry with a nitrogen blowing instrument;

[0036] (4) The precipitate is retained in the centrifuge tube, continue to add 5mL 80% ethanol → vortex oscillator → rotary mixer for 30 minutes → vortex oscillator → rotary mixer for 30 minutes → vortex oscillator → rotary mixer for 30 minutes (total 1.5h for three times) → 3000r Centrifuge for 5 min;

[0037] (5) Pipette the supernatant into the blow-dried frozen bottle, blow it dry with a nitrogen blower, and freeze the dryer for 24-48h, wait until it rises to room temperature to weigh, seal the bottle, and store at -20°C.

[0038]

[0...

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Abstract

The invention relates to the field of rumen regulation and control, in particular to a natural product preparation for promoting the rumen fermentation. The natural product preparation is prepared through the following steps: after grinding homalomena occulta roots, extracting with 60-80 mass percent of ethanol solution, and performing nitrogen flushing and freeze drying to obtain dry powder. Through researching, the inventor has discovered that the homalomena occulta roots have a certain effect on promoting the rumen fermentation. Homalomena occulta has a wide source property and cooperativity, the experiment through adopting a filter paper flat band method shows that obscured homalomena rhizome volatile oil has a function of inhabiting bacterium burgeri on flocks and herds. Therefore, the homalomena occulta root extractive preparation is hopeful to further comprehensively improving the rumen function and promoting the health of ruminants after scientific compatibility.

Description

technical field [0001] The invention relates to the field of rumen regulation, in particular to a natural product preparation for promoting rumen fermentation. Background technique [0002] With the development of the national economy and the gradual change of people's dietary structure, the development of herbivorous livestock has become one of the main directions for the development of animal husbandry in my country in the future. Grass-fed livestock breeding is a world-recognized food-saving, economical and efficient animal husbandry, and is an important part of modern agriculture. Statistics show that at the end of 2010, the number of dairy cows in my country was about 12.5 million, an increase of 120.8% over the 5.66 million in 2001, while the milk output was 35.7 million tons, an increase of 248.3% over the 10.25 million tons in 2001. The dairy farming industry has developed rapidly, and the scale, science, standardization, and mechanization have all been significantl...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/16
Inventor 王佳堃刘美刘建新
Owner ZHEJIANG UNIV
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