Preparation method for amorpha fruticosa glycosides

A technology of amorphoside and amorphaside, which is applied in the field of natural medicinal chemistry and can solve the problems of disclosure and no industrialization method of amorphaside

Inactive Publication Date: 2013-12-04
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Through literature search, there is n...

Method used

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  • Preparation method for amorpha fruticosa glycosides

Examples

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Embodiment 1

[0017] Take 5kg of raw materials of Amorpha fruticosa fruit and crush them, add 1000ml of water and 10g of amylase to enzymatically hydrolyze for 20 hours, add 7 times the amount of 70% ethanol solution to the enzymatically hydrolyzed raw materials and extract twice, dilute the concentrated solution with appropriate amount of water and add ADS-21 macropore Adsorbed in the resin, first use 5 times the column volume of 40% ethanol solution to elute the impurities, then use 10 times the column volume of 50% ethanol to elute the active ingredient, add an appropriate amount of 30% ethanol solution to dissolve the concentrated solution, and add it to ADS-21 macroporous resin Adsorption, elute impurities with 6 times column volume of 30% ethanol solution, then elute with 8 times column volume of 55% ethanol, collect the eluate and concentrate it under reduced pressure until it is anhydrous and place it to crystallize, filter the crystals and dissolve the weight in ethyl acetate Crysta...

Embodiment 2

[0019] Take 5kg of Amorpha fruticosa raw material and crush it, add 1000ml of water and 13g of cellulase to enzymolyze it for 10 hours, add 6 times the amount of 60% ethanol solution to the enzymatic hydrolysis raw material, reflux and extract it for 3 times, dilute the concentrated solution with appropriate amount of water and add AB-8 large Adsorbed in the porous resin, first use 10 times column volume 20% ethanol solution to elute impurities, then use 7 times column volume 70% ethanol to elute active ingredients, add appropriate amount of 30% ethanol solution to dissolve the concentrated solution, add AB-8 macroporous resin Adsorb in medium, elute impurities with 6 times column volume of 30% ethanol solution, then elute with 8 times column volume of 55% ethanol, collect eluate and concentrate under reduced pressure until anhydrous and place crystallization, filter crystals and dissolve them in ethyl acetate Recrystallized and dried at low temperature to obtain 28 g of a...

Embodiment 3

[0021] Take 5kg of raw materials of Amorpha fruticosa fruit and crush them, add 1000ml of water and 20g of pectinase to enzymatically hydrolyze for 5 hours, add 7 times the amount of 90% ethanol solution to the enzymolyzed raw materials and extract them for 3 times, dilute the concentrated solution with appropriate amount of water and add HZ816 macroporous resin Adsorption in medium, first use 6 times column volume 30% ethanol solution to elute impurities, then use 6 times column volume 70% ethanol to elute active ingredients, add appropriate amount of 30% ethanol solution to dissolve the concentrated solution, add it to HZ816 macroporous resin for adsorption, use 6 times the column volume of 30% ethanol solution to elute the impurities, and then 8 times the column volume of 55% ethanol to elute, collect the eluate and concentrate it under reduced pressure until it is anhydrous and place it to crystallize, the crystals are filtered out and recrystallized with ethyl acetate. ...

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Abstract

The invention discloses a preparation method for amorpha fruticosa glycosides. The method includes the following steps that (1) amorpha fruticosa fruit raw material is taken and smashed, biological enzymes are added to carry out enzymolysis, the raw material after the enzymolysis is added to a 60-90% ethanol solution and is subjected to reflux extraction for 2-3 times, wherein the amount of the ethanol solution is 5-10 times that of the raw material, an extracted solution is filtered and concentrated, a concentrated solution is diluted by adding appropriate amount of water and then adsorbed through macroporous resin, gradient elution is carried out through an ethanol solution, and an eluant is concentrated to obtain a concentrated solution; (2) the concentrated solution is diluted by adding appropriate amount of water and added to the macroporous resin for adsorption, a 30% ethanol solution with the volume six times of a column volume, then 55% ethanol with the volume eight times of the column volume is used for elution, the eluant is collected, concentrated in a decompression mode to a small volume and stands for crystallization, the crystal substance is subjected to recrystallization through ethyl acetate, and the amorpha fruticosa glycosides are obtained after low temperature drying. The preparation method for the amorpha fruticosa glycosides is simple in technological operation, low in production cost and easy to produce industrially.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and in particular relates to a preparation method of amorphoside. Background technique [0002] Amorphaside is a flavonoid substance, CAS No. 4207-90-3, molecular weight C34H40O16, molecular weight 704.6, molecular structure formula: [0003] [0004] Amorphoside is a white needle-like crystal with a melting point of 154-155°. Amorphoside exists in the fruit of Amorpha fruticosa, and has the functions of inhibiting phosphodiesterase, inhibiting hypercoagulation and protecting the liver. [0005] Through literature search, no industrialization method of amorphoside has been disclosed yet. Contents of the invention [0006] The technical problem to be solved by the present invention is to provide a method for preparing amorphoside which is easy for industrial production. [0007] The present invention is achieved through the following technical solutions: [0008] A kind of prepa...

Claims

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Application Information

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IPC IPC(8): C07H15/26C07H1/08
Inventor 刘东锋万冬梅
Owner NANJING ZELANG MEDICAL TECH
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