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Composition for preventing necrotizing enterocolitis and use thereof

A composition, the technology of Lactobacillus plantarum, applied in the field of biomedicine, can solve problems such as high risk, harming probiotics, and destroying the balance of intestinal bacteria

Inactive Publication Date: 2013-12-04
PROMD BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, surgery is very risky for premature newborns. Although antibiotic treatment can inhibit the growth of pathogenic bacteria in the intestinal tract, it will also damage other beneficial bacteria in the intestinal tract and disrupt the balance of intestinal bacteria

Method used

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  • Composition for preventing necrotizing enterocolitis and use thereof
  • Composition for preventing necrotizing enterocolitis and use thereof
  • Composition for preventing necrotizing enterocolitis and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Screening and isolation of Lactobacillus plantarum PM-A0087 strain, Lactobacillus longum PM-A0101 strain and Lactobacillus bifidobacterium PM-A0218 strain

[0035] Lactobacillus plantarum PM-A0087 strain

[0036] Put the commercially available kimchi into a 50ml centrifuge tube, add a little sterile deionized water, and drop into the following various culture media for different separation purposes:

[0037] (1) MRS-seal: isolate Lactobacillus-facultative anaerobic environment;

[0038] (2) MRS-unsealed: Separation of cocci-aerobic environment;

[0039] (3) MRS+cysteine ​​(Cysteine)-sealed (anaerobic): isolate B bacteria of Lactobacillus-facultative anaerobic environment;

[0040] (4) LBS-seal: isolate Lactobacillus-facultative anaerobic environment;

[0041] (5) LBS+glacial acetic acid-sealed (anaerobic): isolate Lactobacillus-facultative anaerobic environment;

[0042] (6) TS-unsealed: isolate bacillus-aerobic environment;

[0043] (7) LB-unsealed: iso...

Embodiment 2

[0067] Example 2: The effect of a composition comprising one of Lactobacillus plantarum PM-A0087 strain, Lactobacillus longum PM-A0101 strain and Lactobacillus bifidobacterium PM-A0218 strain and a combination thereof for preventing necrotic enteritis.

[0068] Strain culture

[0069] Lactobacillus plantarum PM-A0087 strain was cultured in MRS medium at 37°C, Bifidobacterium longum PM-A0101 strain and Bifidobacterium bifidobacterium PM-A0218 strain were cultured in MRS medium with 5% cysteine ​​in an anaerobic environment , Culture at 37°C. After cultivating for three days, each lactic acid bacteria strain was taken out from the incubator, and then the bacterial solution in the culture tube was repeatedly sucked and completely dispersed, and the absorbance value was measured and the number of bacteria was estimated according to the measured value, and Lactobacillus plantarum PM- The bacterial counts of A0087 strain, Lactobacillus longum PM-A0101 strain and Lactobacillus bifid...

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Abstract

The invention discloses a composition for preventing necrotizing enterocolitis and a use thereof, and belongs to the field of biological medicine. The composition of the invention includes selecting from one or more separated lactobacillus strains, wherein the lactobacillus strains are selected from a group composed of lactobacillus plantarum subsp.plantarum PM-A0087 strains (preserved in NRRL, with a preservation number of NRRL-B50523), bifidobacterium longum PM-A0101 strains (preserved in NRRL, with a preservation number of NRRL-B50525) and bifidobacterium bifidum PM-A0218 strains (preserved in NRRL, with a preservation number of NRRL-B50524). The invention also discloses the use of the composition containing any one of the lactobacillus strains or combinations of the lactobacillus strains in preparation of drugs for preventing necrotizing enterocolitis.

Description

technical field [0001] The invention relates to a lactobacillus strain and a composition prepared therefrom, and also relates to the use of the composition in preparing medicines for preventing necrotic enteritis. The invention belongs to the field of biomedicine. Background technique [0002] Necrotizing enterocolitis (NEC) is a common intestinal disease with high mortality in premature infants. Necrotizing enteritis occurs in about 12% of premature infants with a birth weight of less than 1,500 grams. Symptoms include loss of appetite, weight loss, abdominal distention, bloody stools, peritonitis, sepsis, and shock, which can lead to death in severe cases. Currently known related pathogenic factors include premature birth, intestinal wall ischemia / hypoxia, bacterial infection, and intestinal feeding, etc., but the real etiology remains unclear. [0003] Treatment for necrotizing enteritis includes fasting, supportive care, antibiotics, and surgery. However, surgery is v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P1/00A23L1/30A23L2/52C12R1/25C12R1/01A23L33/135A61K35/747
Inventor 苏伟志李光智陈湘陵吴小俐
Owner PROMD BIOTECH
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