Culture method of ellcalyplus grandis Hill ex Maid tissue culture seedling

A method for cultivating tissue culture seedlings, applied in the field of cultivating Eucalyptus grandis tissue culture seedlings, can solve problems such as environmental pollution, short lifespan, mercury content, etc., so as to improve the callus induction rate, increase the average cost less, and shorten the emergence of seedlings. effect of time

Inactive Publication Date: 2015-07-22
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, incandescent lamps are the main light source for tissue culture, but incandescent lamps have high energy consumption, short lifespan, contain mercury, are easy to cause environmental pollution, and have a single light quality, which cannot meet the different light quality requirements of plants in different growth stages of tissue culture.

Method used

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  • Culture method of ellcalyplus grandis Hill ex Maid tissue culture seedling
  • Culture method of ellcalyplus grandis Hill ex Maid tissue culture seedling
  • Culture method of ellcalyplus grandis Hill ex Maid tissue culture seedling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Select mature seeds and soak them in warm water at 50-55°C and cool them down naturally. After 4-6 hours, wash them twice with sterile water, sterilize them with 20% (volume ratio) NaClO for 15 minutes, rinse them with sterile water for 3-4 times, and inoculate them in 1 / 2 MS culture. basically.

[0014] Take the leaves of the above-mentioned tissue-cultured seedlings (leaf width 0.5-1 cm) as explants, and cut 3-4 wounds on the leaves, and the depth reaches the main vein. Insert the treated leaves into WPM+6-BA1mg L -1 +NAA0.2mg·L -1 In the culture medium, after 5 days of dark culture, 7 LED light sources with different light qualities were placed to induce callus, and 9 petri dishes with a size of 90*15mm were placed under each light, and 12 leaves were inoculated in each petri dish. Adjust the LED light control software and the distance between the light source and the plants so that the light intensity is 105 μmol m -2 ·s -1 around, the photoperiod is 16h·d -1 ,...

Embodiment 2

[0024] A batch of sterile seedling leaves (0.5-1 cm in leaf width) were reselected as explants, and callus induction culture was carried out under RB2 light, and the rest of the operation and culture conditions were the same as in Example 1.

[0025] After 40 days, the callus cultured under RB2 light was transferred to WPM+6-BA1mg L-1+NAA0.3mg L-1 medium, and placed under 7 LED light sources with different light qualities in Table 1. For adventitious bud differentiation culture, 9 petri dishes with a size of 90*15mm were placed under each light, and 12 pieces of callus explants were inserted into each petri dish. The light, photoperiod and conditions of the culture room are all consistent with those in Example 1.

[0026] After 30 days of transfer culture, the growth of adventitious shoots of calli under 7 light treatments was counted, and the results are shown in Table 3.

[0027] Table 3 Adventitious buds under different light quality

[0028]

[0029] Note: germination...

Embodiment 3

[0033] The effective adventitious buds cultivated under monochromatic blue light and monochromatic red light in Example 2 were excised, and added 1.0 mg·L -1 In the 1 / 2MS medium of ABT, put it under 7 different light quality LED light sources in Table 1 to carry out adventitious bud growth and rooting culture, put 9 bottles (diameter 7cm, high 10cm glass bottle) under each light, each bottle receives 8 active buds. Adjust the LED light control software and the distance between the light source and the plants so that the light intensity is 95 μmol m -2 ·s -1 around, the photoperiod is 14h·d -1 , cultivated for 20 days. The relative humidity of the culture room is 70%±5%, and the temperature is (24±1)°C.

[0034] After 20 days of cultivation, the growth and rooting of the seedlings cultivated under different light qualities were observed, see Table 4 for details. After observation, the seedlings cultivated under green light were transferred to yellow light for rooting cultu...

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Abstract

The invention discloses a culture method of an ellcalyplus grandis Hill ex Maid tissue culture seedling, which comprises the following operation steps of taking an ellcalyplus grandis Hill ex Maid tissue culture seedling leaf as an explant, inducing a callus tissue of the ellcalyplus grandis Hill ex Maid leaf, and performing adventitious bud differentiation culture and rootage culture on the callus tissue of the leaf, and is characterized in that the callus tissue of the ellcalyplus grandis Hill ex Maid leaf is induced under the irradiation of red light and blue light, the adventitious bud differentiation culture is performed on the callus tissue of the leaf under the irradiation of the red light or the blue light, adventitious bud growth culture is performed under the irradiation of green light, and then the callus tissue is transferred to be under LED (light-emitting diode) yellow light for the rootage culture. According to the method, light sources are adjusted timely according to difference in requirements of the ellcalyplus grandis Hill ex Maid leaf on the light in different tissue culture stages, and the induction rate, the adventitious bud differentiation rate and the rootage rate of the callus tissue are increased effectively. In addition, the method is low in energy consumption, and can greatly increase the yield of the ellcalyplus grandis Hill ex Maid tissue culture seedling and effectively shorten the seedling emergence time in comparison to the traditional tissue culture method.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and in particular relates to a method for cultivating Eucalyptus grandis tissue culture seedlings. Background technique [0002] Eucalyptus grandis (Ellcalyplus grandis Hill ex Maid) is native to Australia. It is a tall tree with the characteristics of fast growth, high yield, salt-alkali resistance, and cold resistance. It is one of the main tree species for the construction of timber forests, coastal shelter forests, and ecological forests. All provinces have established a certain scale of short-rotation industrial timber forest bases. Now, with the development of the western region and the implementation of the Yangtze River and Pearl River shelterbelt projects, the progress of returning farmland to forests is accelerating, and the planting area of ​​Eucalyptus giantis is gradually expanding. broad. The propagation of Eucalyptus giantis mostly adopts the method of tissue culture, and the tech...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 黄华宏林二培程龙军童再康李许可
Owner ZHEJIANG FORESTRY UNIVERSITY
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