Plate culture and preparation method of ustilaginoidea virens thin-wall conidium

Abstract

The invention discloses a plate culture and preparation method of ustilaginoidea virens thin-wall conidium. The core of the method is that the thin-wall conidium is directly utilized for culturing and breeding new thin-wall conidium through a plate culture medium. The method comprises the following steps: 1) preparing an initial thin-wall conidium solution; 3) preparing a sporulation culture medium plate; 3) performing sporulation cultivation; 4) collecting new thin-wall conidium; and 5) purifying conidium. The method has the advantages that the cultivation and preparation can be performed within a shorter time which is generally 4 to 6 days; the operation is simple, easy and convenient; oscillating culture equipment is not required; the thin-wall conidium in an obtained culture product is mainly the conidium of the same generation; the conidium solution is relatively high in quality; no purifying treatment is required; and the method is applied to a plurality of research works.

Description

technical field [0001] The present invention relates to agricultural technology and biotechnology. Specifically, it is a preparation method for plate culture of conidia of rice false smut fungus parenchyma. Background technique [0002] In most fungal disease systems, the propagules of pathogenic bacteria not only have the function of multiplying and expanding their own population, but more importantly, they have the function of interacting with and identifying the host, and initiating the function of infecting the host. Therefore, in many important plant disease research In the process, the propagules of pathogenic bacteria need to be used as test materials. Obviously, an efficient and convenient method for culturing and preparing propagule materials will be very conducive to the smooth development of research work. Rice false smut has become a major disease in rice production in my country. The propagules of rice false smut include three types: sexual ascospores, asexual ...

AI Technical Summary

Problems solved by technology

[0004] These two methods can effectively prepare and obtain the thin-walled conidia of rice false smut, but in practice they all have some disadvantages, such as time-consuming; more labor-intensive; vibration culture equipment is required; A mixture of multi-generation spores of the genus Conidia; the culture product mixture contains a large number of mycelium clusters, bacterial metabolites, and medium components, etc., and it is often necessary to filter out mycelia and precipitate spores before subsequent treatment steps are suitable for use At the same time, because the culture product is in a viscous state, high-speed centrifuge equipment is often required to precipitate spores

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