Plate culture and preparation method of ustilaginoidea virens thin-wall conidium

A technology for conidia and rice smut fungus, which is applied in the fields of agricultural technology and biology, can solve the problems of shaking culture equipment, labor-intensive, time-consuming, etc., and achieves the effect of simple and convenient operation.

Active Publication Date: 2013-12-11
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] These two methods can effectively prepare and obtain the thin-walled conidia of rice false smut, but in practice they all have some disadvantages, such as time-consuming; more labor-intensive; vibration culture equipment is required; A mixture of multi-generation spores of the genus Conidia; the culture product mixture contains a larg...

Method used

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  • Plate culture and preparation method of ustilaginoidea virens thin-wall conidium
  • Plate culture and preparation method of ustilaginoidea virens thin-wall conidium
  • Plate culture and preparation method of ustilaginoidea virens thin-wall conidium

Examples

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Embodiment 1

[0047] Adopt a kind of rice smut fungus parenchyma culture preparation method of the present invention, cultivate and prepare the parenchyma conidia of rice smut fungus bacterial strain Uv-34, operate according to the following steps:

[0048] 1) Preparation of initial parenchyma liquid:

[0049] a) The primary source of parenchyma conidia: using the well-known second-stage culture method, the culture product containing a large number of parenchyma conidia is obtained by means of shaking culture with mycelium;

[0050] b) Spore purification treatment: The obtained culture product containing parenchyma conidia is subjected to spore purification treatment, and the purification treatment method is to remove the hyphae in the culture product by filtering with two layers of gauze; then the filtrate is centrifuged and collected Precipitating spores can remove the medium components and bacterial exudates in the culture products; then suspend and wash the precipitated spores with ster...

Embodiment 2

[0061] A method for culturing and preparing thin-walled conidia of the rice smut fungus strain Uv-105 was adopted to cultivate and prepare the thin-walled conidia of the rice smut fungus strain Uv-105, and the operations were carried out according to step 1) to step 4) of Example 1, and the results were as follows: plate to obtain 30ml of culture product containing parenchyma liquid, and the spore concentration of the culture product reached 2.72×10 6 spore / ml; the appearance character of this culture product is identical with embodiment 1;

[0062] After the operation was carried out according to step 5) of Example 1, very pure parenchyma conidia of bacterial strain Uv-105 were obtained.

Embodiment 3

[0064] A method for culturing and preparing thin-walled conidia of the rice smut fungus strain Uv-110 was adopted to cultivate and prepare the thin-walled conidia of the rice smut fungus strain Uv-110, and the operations were carried out according to step 1) to step 4) of Example 1, and the results were as follows: dish to obtain 30ml of the culture product containing the parenchyma liquid, and the spore concentration of the culture product reached 2.15×10 6 spore / ml; the appearance character of this culture product is identical with embodiment 1;

[0065] After the operation was carried out according to step 5) of Example 1, very pure parenchyma conidia of bacterial strain Uv-110 were obtained.

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Abstract

The invention discloses a plate culture and preparation method of ustilaginoidea virens thin-wall conidium. The core of the method is that the thin-wall conidium is directly utilized for culturing and breeding new thin-wall conidium through a plate culture medium. The method comprises the following steps: 1) preparing an initial thin-wall conidium solution; 3) preparing a sporulation culture medium plate; 3) performing sporulation cultivation; 4) collecting new thin-wall conidium; and 5) purifying conidium. The method has the advantages that the cultivation and preparation can be performed within a shorter time which is generally 4 to 6 days; the operation is simple, easy and convenient; oscillating culture equipment is not required; the thin-wall conidium in an obtained culture product is mainly the conidium of the same generation; the conidium solution is relatively high in quality; no purifying treatment is required; and the method is applied to a plurality of research works.

Description

technical field [0001] The present invention relates to agricultural technology and biotechnology. Specifically, it is a preparation method for plate culture of conidia of rice false smut fungus parenchyma. Background technique [0002] In most fungal disease systems, the propagules of pathogenic bacteria not only have the function of multiplying and expanding their own population, but more importantly, they have the function of interacting with and identifying the host, and initiating the function of infecting the host. Therefore, in many important plant disease research In the process, the propagules of pathogenic bacteria need to be used as test materials. Obviously, an efficient and convenient method for culturing and preparing propagule materials will be very conducive to the smooth development of research work. Rice false smut has become a major disease in rice production in my country. The propagules of rice false smut include three types: sexual ascospores, asexual ...

Claims

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Application Information

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IPC IPC(8): C12N3/00
Inventor 张君成覃茜
Owner GUANGXI UNIV
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