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Preparation method for plant cirrus fermentation liquid glucose

A plant vine and sugar fermentation technology, which is applied in the field of preparation of plant vine fermented sugar liquid, can solve the problems of loss of enzyme activity, low hydrolysis efficiency of crystalline structure hemicellulose structure, etc., achieve low cost, reduce technical risk, and broad market prospects Effect

Active Publication Date: 2013-12-11
TAICANG BIQI NEW MATERIAL RES & DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of enzymatic hydrolysis, due to the irreversible adsorption of some cellulase and hemicellulase on the surface of the substrate, especially the lignin, the enzyme activity will be lost. In addition, the crystal structure of the cellulose itself and the hemifiber The complexity of the protein structure is also an important factor leading to low hydrolysis efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Harvesting watermelon vines, washing them with tap water, drying them, placing the cleaned vines in a grinder, and crushing them into particles with a particle size of 20 to 100 mesh;

[0015] Mix 10 g of crushed vines with 200 mL of 1% ammonium chloride aqueous solution, heat to 60°C, react for 1 hour, cool, and filter to obtain ammonium salt-treated vines;

[0016] Mix 30g of calcium oxalate with 100g of 20% hydrochloric acid solution. After dissolving, add deionized water until the weight of the mixed solution is 1000g to obtain a mixed aqueous solution of calcium oxalate / hydrochloric acid;

[0017] Put 5g of vines treated with ammonium salt in 50mL calcium oxalate / hydrochloric acid mixed aqueous solution, adjust the pH of the solution to 2-3, heat to 80°C in a stainless steel reactor, react for 3 hours, cool, and filter to obtain plant vine fermentation sugar liquid .

[0018] Take out an appropriate amount of sugar solution, boil it, inactivate it, and dilute it a...

Embodiment 2

[0020] Harvesting the pumpkin vines, washing them with tap water, drying them, placing the cleaned vines in a pulverizer, and pulverizing them into particles with a particle size of 20 to 100 mesh;

[0021] Mix 10 g of crushed vines with 600 mL of 5% ammonium sulfate aqueous solution, heat to 80°C, react for 3 hours, cool, and filter to obtain ammonium salt-treated vines;

[0022] Mix 30g of calcium oxalate with 100g of 20% hydrochloric acid solution. After dissolving, add deionized water until the weight of the mixed solution is 1000g to obtain a mixed aqueous solution of calcium oxalate / hydrochloric acid;

[0023] Put 5g of vines treated with ammonium salt in 150mL calcium oxalate / hydrochloric acid mixed aqueous solution, adjust the pH of the solution to 2-3, heat to 120°C in a stainless steel reactor, react for 6 hours, cool, and filter to obtain plant vine fermentation sugar liquid ;

[0024] Take out an appropriate amount of sugar solution, boil it, inactivate it, and di...

Embodiment 3

[0026] Harvest sweet potato vines, wash them with tap water, dry them, put the cleaned vines in a grinder, and grind them into particles with a particle size of 20 to 100 mesh;

[0027] Mix 10 g of crushed vines with 500 mL of 3% ammonium nitrate aqueous solution, heat to 70°C, react for 2 hours, cool, and filter to obtain ammonium salt-treated vines;

[0028] Mix 30g of calcium oxalate with 100g of 20% hydrochloric acid solution. After dissolving, add deionized water until the weight of the mixed solution is 1000g to obtain a mixed aqueous solution of calcium oxalate / hydrochloric acid;

[0029] Put 5g of vines treated with ammonium salt in 100mL calcium oxalate / hydrochloric acid mixed aqueous solution, adjust the pH of the solution to 2-3, heat to 120°C in a stainless steel reactor, react for 6 hours, cool, and filter to obtain plant vine fermentation sugar liquid ;

[0030] Take out an appropriate amount of sugar solution, boil it, inactivate it, and dilute it appropriately...

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Abstract

The invention belongs to the field of biomass energy, and relates to a preparation method for a plant cirrus fermentation liquid glucose. The preparation method provided by the invention hydrolyzes the cirrus, and particularly comprises the technologies of cirrus cleaning, smashing, ammonium salt processing, calcium oxalate catalyzing and hydrolyzing and the like. The preparation method for the plant cirrus fermentation liquid glucose provided by the invention has the following advantages: (1) the cost is low, and the raw material source is wide; (2) the preparation technology is simple, the input is less, and large-scale popularization and application are facilitated; (3) the uncertainty of the microorganism enzyme fermentation sugar refining technology is avoided, and the technical risk is reduced; (4) the sugar conversion rate is high, the produced residue quantity is less, and the preparation method is an environment friendly technology. The plant cirrus fermentation liquid glucose prepared by the invention can be used as a raw material for producing alcohol, and the market prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of biomass energy, and in particular relates to a method for preparing sugar liquid fermented by plant vines. Background technique [0002] With the growth of population, food, energy and environment are the most important challenges facing human beings. The production of renewable clean energy from cellulosic raw materials has attracted increasing attention, and the production of fuel ethanol from crop wastes is considered to be the most promising industry. Economic crops such as watermelons, pumpkins, sweet potatoes, and lentils are planted in a wide area in my country, and almost the same amount of vines are produced every year when the fruits are harvested. The protein content in the vine is low but the carbohydrate content is high, and the carbohydrate content of individual varieties is as high as 82% of the dry matter, so it is a high-quality raw material for the production of biomass energy. Researc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10
CPCY02E50/10
Inventor 蓝碧健
Owner TAICANG BIQI NEW MATERIAL RES & DEV
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