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A kind of rapid determination method of peracetic acid residual

A peracetic acid, rapid determination technology, applied in the field of biotechnology enzymatic analysis, can solve problems such as lack of detection of peracetic acid residues, and achieve the effects of saving test costs, ensuring accuracy, and simple testing process

Active Publication Date: 2015-12-09
GUANGDONG HUANKAI MICROBIAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is still a lack of widely recognized methods for the detection of peracetic acid residues, so it is imminent to develop a convenient, high-sensitivity, and reliable peracetic acid residue testing product

Method used

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  • A kind of rapid determination method of peracetic acid residual

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Prepare with pure water containing 0.04g / L horseradish peroxidase, 1.5g / LTMB hydrochloride, 0.037mol / L citric acid, 0.126mol / L disodium hydrogen phosphate, 10g / L trehalose, 5g / L glycine , 3g / LBSA, 10g / LPEG4000 mixed color solution. Soak the chromatographic filter paper completely in the above mixture for about 10 minutes, take out the filter paper, and dry it under vacuum at a temperature lower than 30°C. One end of the bottom plate is made of peracetic acid residue rapid determination test paper 1#.

Embodiment 2

[0038] Prepare with pure water containing 0.01g / L horseradish peroxidase, 1.0g / LTMB sulfate, 0.028mol / L citric acid, 0.11mol / L disodium hydrogen phosphate, 10g / L trehalose, 5g / L arginine A mixed chromogenic solution of acid, 5g / LBSA, 10g / LPVP. Soak the chromatographic filter paper completely in the above mixture for 5 minutes, take out the filter paper, and dry it under vacuum at a temperature lower than 30°C. Cut the filter paper into small pieces of 5×5mm and stick them on the strip-shaped bottom plate of 5×50mm with glue One end is to make peracetic acid residue rapid determination test paper 2#.

Embodiment 3

[0040]Prepare with pure water containing 0.025g / L horseradish peroxidase, 1.25g / LTMB propanesulfonate, 0.044mol / L citric acid, 0.14mol / L disodium hydrogen phosphate, 2g / L sucrose, 2g / L seaweed Mixed chromogenic solution of sugar, 2g / L glycine, 1g / LHSA, 4g / LPEG6000. Soak the qualitative material completely in the above mixture for about 20 minutes, take out the filter paper, and dry it under vacuum at a temperature lower than 30°C. Cut the filter paper into small pieces of 5×5 mm, and glue them on a strip of plastic hardwood with a size of 5×50 mm. One end of the plate is made into 3# rapid determination test paper for peracetic acid residue.

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Abstract

The invention discloses a rapid determination method for residual peracetic acid, which is a fast response time, high sensitivity, good stability, simple operation, economical and practical method capable of rapidly detecting the content of residual peracetic acid in a sample on site , the minimum detectable peracetic acid content is 0.5mg / L. The pH regulator in the test paper used in the method of the present invention ensures the accuracy of the test results; the enzyme protective agent in the test paper ensures the stability of the enzyme test paper, and the shelf life can reach at least 12 months; the area of ​​the test paper used is small, and the test process is simple, There is no need for other instruments and accessories, which can greatly save the cost of testing; the bottom plate of the test paper can prevent contamination by human hands during the process of taking it, which is very safe and reliable.

Description

technical field [0001] The invention belongs to the field of biotechnology enzymatic analysis, in particular to a rapid determination method for peracetic acid residue. Background technique [0002] As a new type of high-efficiency and low-toxicity bactericidal disinfectant, peracetic acid is mainly used in the disinfection of food processing equipment, medical equipment and supplies. With the deterioration of the environment, there are more and more kidney disease patients in the world. According to incomplete statistics, the number of hemodialysis patients in my country has exceeded 100,000 in 2008, so the disinfection of peracetic acid in hemodialysis is particularly important. The hemodialysis machine generally needs to be disinfected once every 1-3 months, and 0.2% peracetic acid can be used to circulate for 20 minutes and soak for 30 minutes to disinfect; the water storage tank and pipeline generally need to be disinfected once every 15 days, and 0.2% peracetic acid ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 邓金花秦惠黄报亮吴清平
Owner GUANGDONG HUANKAI MICROBIAL SCI & TECH
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