Preparation method of drug-loaded microsphere/chitosan/sodium alginate injectable hydrogel
A technology of drug-loaded microspheres and sodium alginate, which is applied in the field of preparation of biomedical materials, can solve the problems of low mechanical strength and poor drug release performance, and achieve the effects of enhanced mechanical strength, low cost, and weakened burst release effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0022] (1) Dissolve 0.35g chitosan in 2% 10ml dilute acetic acid aqueous solution to obtain solution 1; dissolve 0.3g sodium alginate in 10ml deionized water at 80°C to obtain solution 2; dissolve 0.03g Tris in 1ml deionized water Ionized water to obtain solution 3; 0.75g anhydrous calcium chloride was dissolved in 3ml deionized water to obtain solution 4;
[0023] (2) Stir the solutions 1, 2, and 3 obtained in step (1) at room temperature at 470rpm to obtain a mixed solution; add 0.105g of drug-loaded microspheres of 300~450um prepared in advance to the mixed solution, and use a homogenizer to Disperse evenly at a high speed of 7000rpm to obtain a suspension, and store in a freezer at 4°C for 2 hours;
[0024] (3) Inject the suspension into the defect site, and after 7 minutes, after initial solidification, inject 2.1ml of solution 4; obtain the drug-loaded hydrogel.
[0025] In vitro drug release curve experiment of composite hydrogel: Add 2 mg of drug-loaded microspheres / c...
Embodiment 2
[0027] (1) Dissolve 0.1g chitosan in 1% 10ml dilute acetic acid aqueous solution to obtain solution 1; dissolve 0.12g sodium alginate in 2ml 70°C deionized water to obtain solution 2; dissolve 0.05g Tris in 10ml deionized water Ionized water to obtain solution 3; 1.5g anhydrous calcium chloride was dissolved in 2ml deionized water to obtain solution 4;
[0028] (2) Stir the solutions 1, 2, and 3 obtained in step (1) at room temperature at 300rpm to obtain a mixed solution; add 0.88g of drug-loaded microspheres of 200~300um prepared in advance to the mixed solution, and use a homogenizer to Disperse evenly at high speed at 2000rpm to obtain a suspension, and store in a freezer at 0°C for 4.5h;
[0029] (3) Inject the suspension into the defect site, and after 3 minutes, after initial coagulation, inject 1.47ml of solution 4; obtain the drug-loaded hydrogel.
[0030] The drug-loaded microspheres / chitosan / sodium alginate injectable hydrogel prepared in this embodiment is tested ...
Embodiment 3
[0032](1) Dissolve 0.18g chitosan in 3% 10ml dilute acetic acid aqueous solution to obtain solution 1; dissolve 0.15g sodium alginate in 15ml deionized water at 60°C to obtain solution 2; dissolve 0.75g Tris in 5ml deionized water Ionized water to obtain solution 3; 3g of anhydrous calcium chloride was dissolved in 6ml deionized water to obtain solution 4;
[0033] (2) Stir the solutions 1, 2, and 3 obtained in step (1) at room temperature at 600rpm to obtain a mixed solution; add 0.6g of drug-loaded microspheres of 100~200um prepared in advance to the mixed solution, and use a homogenizer to Disperse evenly at high speed at 4700rpm to obtain a suspension, and store in a freezer at 8°C for 8 hours;
[0034] (3) Inject the suspension into the defect site, and after 10 minutes, after initial solidification, inject 6ml of solution 4; obtain the drug-loaded hydrogel.
[0035] The drug-loaded microspheres / chitosan / sodium alginate injectable hydrogel prepared in this embodiment is ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More