A method for extracting hostanol from Molleri ochrena
A technology of Moraxella ochracea and hopanol, which is applied in the directions of steroids, organic chemistry, etc., can solve the problem of discovering hopanol and the like, and achieve the effects of simple raw material acquisition, low cost and high purity
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Embodiment 1
[0030] 1) Take Mollerii ochraceae (Qiu Junzhi et al., Discovery of Mollella ochraceae in China. Acta Mycophyta Sinica, 2009.28(1): 148-150) as the material, and pick the bacteria with a size of 1× under sterile conditions Inoculate 2 pieces of 1cm bacterial block into PDB medium (200g potato, 20g glucose, boil in water for 30min, filter through 4 layers of gauze, add distilled water to 1L, divide into 250ml Erlenmeyer flasks, each bottle contains 100ml, in Sterilized under high pressure at 121°C for 20min), cultured continuously at 160r / min and 25°C for 5 days, that is, primary culture, then transferred to PDB medium again according to 10% inoculation amount, and continued at 160r / min, 20°C Under continuous culture for 28 days is the secondary culture;
[0031] 2) Filter the culture solution with a vacuum suction filter to obtain mycelium and bacterial solution. After drying the mycelium at 28°C, grind it into powder, add 1 times the volume (w / v) of ethyl acetate to soak for 1...
Embodiment 2
[0042] 1) Take Mollerella ochraceae as the material, pick 2 pieces of bacteria blocks with a size of 1×1cm under aseptic conditions and inoculate them into PDB medium, and culture them continuously for 8 days at 120r / min and 26°C. Culture, transfer to PDB medium again according to 9% inoculum amount, and continue to culture continuously for 30 days under the conditions of 120r / min and 26°C, that is, secondary culture;
[0043] 2) Filtrate the culture solution with a vacuum filtration device to obtain mycelium and bacterial liquid, dry the mycelium at 32°C and grind it into powder, add 1.5 times the volume (w / v) of ethyl acetate to soak for 12 hours , sonicated for 30 minutes, the soaking solution was rotary steamed to obtain the extract, and ethyl acetate was recovered; the bacterial solution and ethyl acetate were mixed in a volume ratio of 1:1, poured into a separatory funnel, and extracted three times by shaking the flask, each time for 4 minutes, from the bottle Pour out the...
Embodiment 3
[0049] 1) Take Mollerella ochraceae as the material, pick 2 pieces of bacteria blocks with a size of 1×1cm under aseptic conditions and inoculate them into PDB medium, and culture them continuously for 10 days at 140r / min and 28°C. Cultivate, transfer to PDB medium again according to 8% inoculum amount, and continue to cultivate continuously for 25 days under the conditions of 120r / min and 28°C, that is, secondary culture;
[0050] 2) Filtrate the culture solution with a vacuum suction filtration device to obtain mycelium and bacterial liquid, dry the mycelium at 35°C and grind it into powder, add 2 times the volume (w / v) of ethyl acetate to soak for 14 hours , sonicated for 40 minutes, the soaking solution was rotary steamed to obtain the extract, and ethyl acetate was recovered; the bacterial solution was mixed with ethyl acetate in a volume ratio of 1:1, poured into a separatory funnel, and extracted by shaking the flask four times, each time for 5 minutes, from Ethyl aceta...
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