Production method of high-quality microalgae biodiesel

A microalgae biological, high-quality technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of easy implementation, increased yield, and simple operation

Active Publication Date: 2013-12-18
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, research on microalgae biodiesel at home and abroad is mainly focused on how to increase the oil content and how to catalyze the conversion of oil into biodiesel. In the cultivation stage of microalgae, how to improve the oil content while improving The quality of oil has almost become blank

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The algal species Chlorella in the present invention has been preserved in the China Typical Culture Collection Center, its preservation number is CCTCC NO: M209256, and its fermentation medium components are as follows:

[0037] Sea salt 28-34g / L, NaNO 3 350-550mg / L, NaH 2 PO 4 30-80mg / L, Na 2 EDTA10-30mg / L, FeSO 4 ·7H 2 O 2.5-5.0mg / L, CuSO 4 ·5H 2 O 0.01mg / L, ZnSO4 7H 2 O0.03-0.06mg / L, CoCl 2 ·6H 2 O 0.02-0.05mg / L, MnCl 2 4H 2 O 0.2-0.6mg / L, Na 2 Mo 4 2H 2 O 0.1-0.3mg / L, Vitamin B 1 0.006-0.06mg / L, Vitamin B 2 0.00005-0.0005mg / L, biotin 0.00005-0.0005mg / L, and the ratio of nitrogen and phosphorus elements is 15-25;

[0038] The first stage of fermentation conditions: the light quality is white light, the light intensity is 8Klux, the initial pH is 7.0, the ventilation intensity is 0.5VVM, CO 2 The concentration is 1.0%, and the culture temperature is 25°C; when the nitrogen nutrient concentration drops to zero, the first stage of fermentation ends;...

Embodiment 2

[0041] The first stage of fermentation conditions: the light quality is blue light, the light intensity is 6Klux, the initial pH is 7.0, the aeration intensity is 0.5VVM, CO 2 The concentration is 1.0%, and the culture temperature is 25°C; when the nitrogen nutrient concentration drops to zero, the first stage of fermentation ends;

[0042] Fermentation conditions in the second stage: the light quality is blue light, the light intensity is 8Klux, the ventilation intensity is 0.5VVM, CO 2 The concentration is 1.0%, and the culture temperature is 28°C. When the algae liquid turns light yellow or the chlorophyll in the algae drops sharply, the fermentation ends; the oil content reaches 38%, and the CN value is 52.1.

Embodiment 3

[0044] The first stage of fermentation conditions: the light quality is white light, the light intensity is 8Klux, the initial pH is 7.0, the ventilation intensity is 0.5VVM, CO 2 The concentration is 1.0%, and the culture temperature is 24°C; when the nitrogen nutrient concentration drops to zero, the first stage of fermentation ends;

[0045] Fermentation conditions in the second stage: the light quality is blue light, the light intensity is 8Klux, the ventilation intensity is 0.5VVM, CO 2 The concentration is 1.0%, and the cultivation temperature is 30°C. When the algae liquid turns light yellow or the chlorophyll in the algae drops sharply, the fermentation ends; the oil content reaches 40%, and the CN value is 50.1.

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Abstract

The invention relates to a production method of high-quality microalgae biodiesel. The method includes: inoculating Chlorella vulgaris (CCTCC: M209256) seeds into a photobioreactor loaded with a fermentation medium, at a first fermentation stage, controlling the illumination intensity at 8-10Klux, the initial pH at 6.0-7.5, the ventilation intensity at 0.4-0.7VVM, the concentration of CO2 at 0.5-2.0%, and the temperature at 24-26DEG C; at a second fermentation stage, keeping the illumination intensity at 30-70Klux, the ventilation intensity at 0.4-0.7VVM, the concentration of CO2 at 0.5-2.0%, and the temperature at 27-30DEG C, and finishing fermentation when the algal liquid turns into light yellow or the chlorophyll in the algae declines substantially. The algal cells obtained by the method have an oil content of over 35%, a stable CN value of more than 50, and a ratio of unsaturated fatty acid to saturated fatty acid at 1.5-2.4.

Description

technical field [0001] The invention belongs to the fields of environmental protection and new technology energy, and in particular relates to a method for producing high-quality microalgae biodiesel. Background technique [0002] As a substitute for petrochemical diesel, microalgae biodiesel should meet the requirements for the use of petrochemical diesel, that is, its physical and chemical properties should be close to those of petrochemical diesel to ensure its fuel characteristics. The indicators to measure the properties of biodiesel mainly include: density, kinematic viscosity, flash point, slip distance, low temperature performance, carbon residue, ash, cetane number, methanol content, fat content, phosphorus content, glycerin content, glycerin content, iodine value And free fatty acids, etc., wherein the combustion performance of biodiesel is evaluated by cetane number. Combustion performance is one of the most important qualities of biodiesel, while cetane number i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCY02E50/10
Inventor 何皓高振尹继龙唐小红黄和孙洪磊齐泮仑兰玲付兴国胡徐腾
Owner PETROCHINA CO LTD
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