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Gene coding sequence for regulating and controlling organ development of cauliflowers and application of gene coding sequence

A gene coding and cauliflower technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as limited understanding, and achieve the effect of low operating environment requirements, high transformation efficiency, and simple operation process

Active Publication Date: 2013-12-18
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current understanding of the molecular regulation mechanism of cauliflower organ development is very limited.

Method used

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  • Gene coding sequence for regulating and controlling organ development of cauliflowers and application of gene coding sequence
  • Gene coding sequence for regulating and controlling organ development of cauliflowers and application of gene coding sequence
  • Gene coding sequence for regulating and controlling organ development of cauliflowers and application of gene coding sequence

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Experimental program
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Embodiment 1

[0031] 一种在花椰菜器官发育中发挥关键调控作用的基因编码序列,它由序列5’ATGATTCGTGAAATCTCCGGTCTACAAAACGACATCGTAAACATTCAAGAACGCTCTTCAAGCAGCAACAACAAGATCATGGACATGAGAAGAGATATTCGCCGACCAGCTCAAGCTCCTATGATGAGTAAGCAAGAATATTTTCGGACATTGTCGTCTCAGAACAGTCCGAGGAGGCTAATCTCAGCGAGTCACTTCAGTTTGGAGTCAATGGTTGTTCTTGTTGGTCTCACAGCATCGCTCTTGATCCTTCCCTTGATTCTTCCACCGTTGCCTCCTCCTCCGTTCATGCTGCTTCTCATTCCTATTGGGATAATGGTTCTGCTTATGGTTCTTGCTTTAATGCCTTCTTCTTCCTCTTCTTCTTCTTCTTCTAATGCCTTACATGTAACAAGAACTTATATGTAA3’组成(SEQ ID NO:1)。

[0032] The method for cultivating high-biomass transgenic plants using the above-mentioned broccoli organ development regulatory gene coding sequence comprises the steps of:

[0033] (1) Extraction of total RNA from cauliflower and synthesis of first-strand cDNA: Total RNA was extracted using Trizol reagent sold by Shanghai Bioengineering Company. Put about 0.2g of sterile cauliflower seedlings or flowering organs in a mortar, quickly add an appropriate amount of liquid nitrogen for grinding, and grind it into powder,...

Embodiment 2

[0040] 一个在花椰菜器官发育中发挥关键调控作用的基因编码序列,它由序列5’ATGATTCGTGAAATCTCCGGTCTACAAAACGACATCGTAAACATTCAAGAACGCTCTTCAAGCAGCAACAACAAGATCATGGACATGAGAAGAGATATTCGCCGACCAGCTCAAGCTCCTATGATGAGTAAGCAAGAATATTTTCGGACATTGTCGTCTCAGAACAGTCCGAGGAGGCTAATCTCAGCGAGTCACTTCAGTTTGGAGTCAATGGTTGTTCTTGTTGGTCTCACAGCATCGCTCTTGATCCTTCCCTTGATTCTTCCACCGTTGCCTCCTCCTCCGTTCATGCTGCTTCTCATTCCTATTGGGATAATGGTTCTGCTTATGGTTCTTGCTTTAATGCCTTCTTCTTCCTCTTCTTCTTCTTCTTCTAATGCCTTACATGTAACAAGAACTTATATGTAA3’组成。 The method for cultivating high-biomass transgenic plants using gene coding sequences is carried out as follows:

[0041] (1) Cauliflower total RNA extraction and first-strand cDNA synthesis;

[0042] (2) Design and synthesis of primers with restriction sites: use biological software to design primers with XbaI and SacI restriction sites: ARL-S: 5' TCTAGA ATGATTCGTGAAATCTCCG3' (the underline indicates the XbaI restriction site); ARL-A: 5' GAGCTC TTACATATAAGTTCTTGTTACATGT3' (the underline indicates the SacI restriction site), and ...

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Abstract

The invention discloses a gene coding sequence which achieves a key regulation and control effect during organ development of cauliflowers and a method for cultivating high-biomass transgenic plants by using the gene coding sequence. The method comprises the following steps of (1) extracting a main RNA (ribonucleic acid) of the cauliflowers and synthesizing a first chain cDNA (deoxyribonucleic acid); (2) designing and synthesizing a primer with enzyme cutting sites; (3) constructing an overexpression transformation vecter; (4) performing genetic transformation; (5) screening transgenetic positive seedlings; (6) observing the biological characters of transgenetic plants. The biological characters of the purified transgenetic plants are observed, and a result shows that the number of leaves of the transgenetic plants is obviously increased and the number of the transgenetic plants is obviously increased; the biomass is about 200 percent of that of wild contrasts. The method for cultivating the high-biomass transgenic plants by using the gene coding sequence disclosed by the invention has an important value for indication of a molecular basis for regulating and controlling the organ development of the cauliflowers, intervention with the organ development progress of the plants through gene engineering measures and cultivation of high-yield crops or economical plants which can fulfill a breeding aim.

Description

technical field [0001] The invention belongs to the technical field of modern molecular biological transgenic plants, and relates to a gene coding sequence in biotechnology that plays a key regulatory role in the development of cauliflower organs and its application in cultivating high-biomass transgenic plants. Background technique [0002] In nature, there are significant differences in organ size between different plant species, but very small differences in organ size among different plant individuals of the same genotype within the same species, indicating that the development of plant organ size is strictly genetically controlled. Organ size is an important feature of plant morphology, and it is also a valuable agronomic trait, such as seed size and fruit size, which directly affect the yield and quality of crops or commercial crops. Therefore, revealing the regulatory factors involved in the development of plant organ size and their mechanism of action has great appli...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82
Inventor 王春国李慧陈成彬宋文芹
Owner NANKAI UNIV
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