Method for preparing cadmium sulfide-coated zinc oxide nano array composite material and method for detecting multiple tumor cells

A zinc oxide nanometer and composite material technology is applied in the field of preparing cadmium sulfide-coated zinc oxide nanometer array composite materials, which can solve the problems of complicated operation, long analysis and detection time, etc., and achieves simple operation, strong application prospect and good effect. Effect

Inactive Publication Date: 2013-12-18
HARBIN INST OF TECH
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Problems solved by technology

However, the above methods usually have the disadvantages of long analysis and detection time and cumbersome operation.

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  • Method for preparing cadmium sulfide-coated zinc oxide nano array composite material and method for detecting multiple tumor cells

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Embodiment Construction

[0016] The technical solution of the present invention will be further described below in conjunction with specific embodiments, but it is not limited to this. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the present invention within the scope of protection.

[0017] Taking HepG2 liver cancer cells as an example, the method for highly sensitive detection of liver cancer cells based on nanocomposite array materials of the present invention includes the following steps:

[0018] (1) Synthesis of nano-zinc oxide array materials: ZnO nanorod arrays were prepared by hydrothermal method, and the ITO (indium tin oxide conductive glass) substrate was immersed in zinc nitrate hexahydrate (0.05~0.07 mol / L) and hexamethylene In a mixed solution of tetramine (0.05 ~0.07 mol / L) (15~20 mL) (the volume ratio of zinc nitrate hexah...

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Abstract

The invention discloses a method for preparing a cadmium sulfide-coated zinc oxide nano array composite material and a method for detecting multiple tumor cells and relates to a method for preparing the cadmium sulfide-coated zinc oxide nano array composite material and a method of the composite material. Preparation of a nano array material is realized according to chemical deposition, modification and assembly of a nanometer material are realized through the electrostatic interaction, and finally, the synthesized nano array material is applied to preparing a cancer cell biosensor. Moreover, the hepatoma cells are sensitively detected, and the method has the advantages of simplicity in operation, high reaction speed, good effect, high selectivity, low cost and the like. The method also can realize detection of multiple tumor cells by changing the types of the modified antibodies, can be used in aspects such as scientific research and clinical medicine and particularly has wide application prospects in the field of biological analysis and in the aspects such as tumor cell detection.

Description

technical field [0001] The invention relates to a method for preparing a zinc oxide nano-array composite material coated with cadmium sulfide and a method for applying the material to detect HepG2 liver cancer cells (or other target tumor cells). Background technique [0002] Zinc oxide is an important wide-bandgap semiconductor material with good optical and electrical properties, and has broad application prospects in high-tech fields such as sensors. Ordered ZnO nanoarrays are ideal optical materials due to their high specific surface area and fast electron transport capability. Cadmium sulfide is another commonly used semiconductor material, which has good photoelectric properties and can be used in electrochemiluminescence immunoassays. [0003] Cancer is one of the important diseases that threaten human life, and liver cancer is a relatively common type of cancer. Therefore, how to detect and diagnose liver cancer early has become one of the research hotspots all over...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76B82Y30/00B82Y15/00
Inventor 韩晓军王磊马生华刘丹青王雪靖
Owner HARBIN INST OF TECH
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