A kind of propagation method of Hemerocallis grandiflora
A technology for Hemerocallis grandiflorum and flower moss, which is applied in the field of using tissue culture technology to propagate Hemerocallis grandiflora, to achieve a stable production system and meet the effect of large-scale rapid propagation production
Active Publication Date: 2015-10-07
浙江乡伴萱草生态农业科技有限公司
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Problems solved by technology
The above methods can meet the production needs, but for the excellent new varieties bred in Hemerocallis grandiflora hybrid breeding, if they are to be popularized in a large area, the annual reproduction coefficient of division propagation is 5-10. It takes at least 6-8 years for the new breeds bred to reach a certain scale.
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Embodiment 1
[0038] Such as figure 1 As shown, a method for propagation of Hemerocallis grandiflora adopts tissue culture technology and specifically includes the following steps:
[0039] (1) Cut the young shoot tips or moss of Hemerocallis grandiflora as tissue culture explants. The said Hemerocallis grandiflora is diploid or polyploid, and the collected explants are mixed with detergent and placed under running water. Rinse for 30 minutes, sterilize with 75% alcohol on a clean bench for 30 seconds, rinse with sterile water for 3-5 times, then use 0.1% mercury to disinfect the young shoot tips for 12-14 minutes and the young flower moss for 8-10 minutes .
[0040] (2) Inoculate the sterilized explants on the bud induction medium (see figure 1 A), sent to the culture room for aseptic culture, the culture temperature of the culture room is 25℃, the light time is 12h·d -1 ,Light intensity is about 25-30μmol·m -2 ·S -1 In this process, according to the differentiation of explants on different c...
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The invention discloses a method for propagating hemerocallis hybridus. The method for propagating the hemerocallis hybridus comprises the following steps: (1) selecting an explant and sterilizing; (2) inoculating the sterilized explant onto a bud induction medium, and carrying out bud induction culture in a culture room; (3) transferring a differentiated explant onto a subculture medium and carrying out subculture, so as to obtain a differentiated seedling; (4) inoculating the differentiated seedling onto a rooting culture medium and carrying out rooting culture; (5) hardening the differentiated seedling, moving the differentiated seedling out of the culture room, and transplanting, wherein the hemerocallis hybridus is a diploid or multiploid. The method for propagating the hemerocallis hybridus is obviously better than other rapid plant tissue culture propagation methods in the aspects of sterilization time, sterilization effect, operation method, regeneration efficiency, production cost and the like, so that an established large-scale commodity production system is stable and the large-scale rapid propagation production can be realized; the method for propagating the hemerocallis hybridus is mainly applied to rapid propagation of new hemerocallis hybridus varieties and is an effective approach for enabling the new hemerocallis hybridus varieties to be rapidly put into the market, so that the current situation that new varieties are pushed over-slowly in China can be changed.
Description
Technical field [0001] The present invention relates to a method for breeding Hemerocallis grandiflora, in particular to a method for breeding Hemerocallis grandiflora using tissue culture technology. Background technique [0002] Hemerocallis hybridus (Hemerocallis hybridus) is rich in germplasm resources and has a wide range of functions. The market demand for it is continuously increasing, especially as an ornamental plant. It has a wide variety of beautiful flowers, green leaves, rich colors, and a long flowering period. It is an excellent garden green flower that integrates leaf viewing and flower viewing. It is generally loved by people all over the world. Because it has the characteristics of easy reproduction, extensive management, strong adaptability and resistance, it meets the requirements of building an economical garden in my country. In garden planting, Hemerocallis can be planted in clusters, rows or rows in flower beds, flower borders, roadsides, lawns, etc. Slice...
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IPC IPC(8): A01H4/00
Inventor 赵天荣蔡建岗
Owner 浙江乡伴萱草生态农业科技有限公司