Biological extracting and purifying method for beer yeast soluble 1,3-beta-D-glucan

A technology of brewer's yeast and purification method, applied in the field of glucan, can solve the problems such as the lack of continuity in the process

Inactive Publication Date: 2013-12-25
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, for the extraction of soluble 1,3-β-D-glucan in yeast cell walls, acid and alkali are generally used to prepare insoluble glucan, and then solubilized by enzymatic or chemical modification methods. The process does not have continuity

Method used

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  • Biological extracting and purifying method for beer yeast soluble 1,3-beta-D-glucan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045]Step 1: Take 120g of fresh yeast sludge from the bottom of the fermenter, wash it repeatedly with excess distilled water, and centrifuge at 4000 rpm for 20 minutes until the supernatant is colorless and clear, and no sugar can be detected by the phenol-sulfuric acid method;

[0046] Step 2: Add water to 5L, the solid content is 2%, stir, put the ultrasonic generator of the ultrasonic cell pulverizer into the mixed solution, the ultrasonic power is 400W, the working time is 5 seconds, the interval time is 10 seconds, and the total ultrasonic time is 20 minutes , to break the wall;

[0047] Step 3: Continue to replenish 5L of water for dilution, adjust the pH value to 6.0, boil and cool to 48°C, add mannanase at 0.5% of the dry weight of yeast, add neutral protease at 200U / g, and react for 24 hours;

[0048] Step 4: Adjust the pH to 5.0, add β-glucanase at 0.5% of the dry weight of the yeast, take 30% after 4 hours of reaction, centrifuge, collect the supernatant, return t...

Embodiment 2

[0052] Step 1: Take 535g of fresh yeast sludge from the bottom of the fermenter, wash it repeatedly with excess distilled water, centrifuge at 4000 rpm for 20 minutes, until the supernatant is colorless and clear, and no sugar can be detected by the phenol-sulfuric acid method;

[0053] Step 2: Add water to 5L, the solid content is 9%, stir, put the ultrasonic generator of the ultrasonic cell pulverizer into the mixed liquid, the ultrasonic power is 500W, the working time is 7 seconds, the interval time is 10 seconds, and the total ultrasonic time is 25 minutes , to break the wall;

[0054] Step 3: Continue to replenish 5L of water for dilution, adjust the pH value to 7.0, boil and cool to 50°C, add mannanase at 1% of the dry weight of yeast, add neutral protease at 210U / g, and react for 26 hours;

[0055] Step 4: Adjust the pH to 5.0, add β-glucanase according to 1% of the dry weight of the yeast, take 30% after 4 hours of reaction, centrifuge, collect the supernatant, return...

Embodiment 3

[0059] Step 1: Take 890g of fresh yeast sludge from the bottom of the fermenter, with a solid content of 15.84%, wash repeatedly with excess distilled water, and centrifuge at 4000 rpm for 20 minutes until the supernatant is colorless and clear, and no sugar can be detected by the phenol-sulfuric acid method ;

[0060] Step 2: Add water to 5L, make the solid content 15%, stir, put the ultrasonic generator of the ultrasonic cell pulverizer into the mixed liquid, ultrasonic power 600W, working time 10 seconds, interval time 10 seconds, total ultrasonic time 30 minutes , to break the wall;

[0061] Step 3: Continue to replenish 5L of water for dilution, adjust the pH value to 8.0, boil and cool to 52°C, add mannanase at 1.5% of the dry weight of yeast, add neutral protease at 250U / g, and react for 28 hours;

[0062] Step 4: Adjust the pH to 5.0, add β-glucanase at 1.5% of the dry weight of the yeast, take 30% after 4 hours of reaction, centrifuge, collect the supernatant, return...

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Abstract

The invention relates to a biological extracting and purifying method for beer yeast soluble 1,3-beta-D-glucan. An existing process for extracting soluble 1,3-beta-D-glucan in yeast cell walls is short of continuity. According to the method, in the same reaction system, yeast cells are degraded by ultrasonic wave, neutral protease, mannose and beta-glucanase, and soluble 1,3-beta-D-glucan with the molecular weight of 10 to 50kD is extracted by an ultrafiltration film with molecular cutoff of 10 to 50kD, so as to obtain soluble 1,3-beta-D-glucan with the molecular weight of 10 to 50kD. The method has the advantages of short technological process, high safety in production and fixed range of the molecular weight of a product; the obtained product has the characteristics of high solubility, good stability and high safety, and is suitable for the fields of food, medicines, cosmetics, pesticides, new materials, electronics, environmental protection and the like.

Description

technical field [0001] The invention relates to a glucan, in particular to a method for bio-refining and purifying brewer's yeast soluble 1,3-beta-D-glucan. Background technique [0002] Glucan is a polysaccharide composed entirely of α-D-glucopyranose monomers, widely distributed in plants, fungi, and bacteria, and is the main skeleton of the cell wall structure. The β-glucan in the cell wall of yeast is the first glucan with immune activity discovered by humans. In subsequent studies, it was found that it also has anti-infection, anti-tumor, anti-radiation and promotion of wound healing, etc. function and is an important biological effect responder. [0003] The 1,3-β-D-glucan in the yeast cell wall occupies the glucan structure, which is connected by a β-1,3 glycosidic bond to another glucose group at the C6 position of the D-glucosyl group, and at the β-1, The main chain connected by 3 glycosidic bonds has small β-1,6 glycosidic bond connected branches, of which β-1,6...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/04
Inventor 李皎杨国武汪大敏邓媛
Owner MICROBIOLOGY INST OF SHAANXI
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