Arabinogalacto-oligosaccharide and preparation and application thereof

A technology of arabinogalactan and galactooligosaccharides, which is applied in the fields of arabinogalactan and galactooligosaccharides and its preparation and application, can solve the problems of uncontrollable broken glycosidic bond position and less research, and achieve obvious promotion in vitro Growth effect, wide application prospect, effect of molecular weight reduction

Active Publication Date: 2020-01-31
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods cannot be directly used for the separation and purification of arabinogalactooligosaccharides
[0004] The existing degradation methods of polysaccharides cannot control the position of the broken glycosidic bonds, which brings about the problem that the molecular weight distribution of the degraded polysaccharides is wide, so that the degraded products may contain polysaccharides with the target molecular weight and the molecular weight produced by excessive degradation. Smaller polysaccharides and oligosaccharides and some polysaccharides with larger molecular weights, so it is necessary to establish a method to obtain functional oligopolysaccharides
[0005] In addition, most of the existing reports on polysaccharides are about the primary structure of polysaccharides, and there are few studies on the advanced structure analysis of polysaccharides, such as the research on the composition of monosaccharides and glycosidic linkages; more and more Studies have shown that the important functions of polysaccharides are determined by their structural characteristics, and their higher-level structures (secondary and tertiary structures) are closer. The biological activity of polysaccharides is closely related to their molecular weight and molecular chain conformation. Molecular chain conformation, etc. are more helpful to clarify the mechanism of its biological activity

Method used

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  • Arabinogalacto-oligosaccharide and preparation and application thereof
  • Arabinogalacto-oligosaccharide and preparation and application thereof
  • Arabinogalacto-oligosaccharide and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Preparation of Arabinogalactooligosaccharides:

[0047] (1) Degradation: Take 0.1g of arabinogalactan in a 100ml hydrolysis bottle, add 20mL of 0.05mol / L citric acid buffer in the hydrolysis bottle, add β- 1,3-galactosidase (that is, add 10Uβ-1,3-galactosidase per gram of arabinogalactan), add distilled water to make the total volume of enzymolysis 50mL, adjust the pH value to 5.0, in 200 rpm, 45 ° C in a constant temperature shaker for 30 h, centrifuged to remove the precipitate, to obtain the supernatant; continue to add α-arabinosidase (i.e. Add 65Uα-arabinosidase per gram of arabinogalactan), add distilled water to make the total volume of enzymolysis 50mL, adjust the pH value to 5.5, enzymolyze in a constant temperature shaker at 200 rpm and 50°C for 30h, centrifuge Remove the precipitate, and finally obtain the degraded supernatant, that is, the degradation solution.

[0048] (2) Membrane separation and purification: Dilute the degradation solution obtained in s...

Embodiment 2

[0052] Preparation of Arabinogalactooligosaccharides:

[0053] (1) Degradation: Take 0.5g arabinogalactan in a 100ml hydrolysis bottle, add 30mL of 0.05mol / L citric acid buffer in the hydrolysis bottle, add β- 1,3-galactosidase (that is, add 45Uβ-1,3-galactosidase per gram of arabinogalactan), add distilled water to make the total volume of enzymolysis 50mL, adjust the pH value to 7.0, in 200 rpm, 55 ° C in a constant temperature shaker for 60 h, centrifuged to remove the precipitate, to obtain the supernatant; continue to add α-arabinosidase (i.e. Add 50Uα-arabinosidase per gram of arabinogalactan), add distilled water to make the total volume of enzymolysis 50mL, adjust the pH value to 7.5, enzymolyze in a constant temperature shaker at 200 rpm and 65°C for 60h, centrifuge Remove the precipitate, and finally obtain the degraded supernatant, that is, the degradation solution.

[0054] (2) Membrane separation and purification: Dilute the degradation solution obtained in step...

Embodiment 3

[0058] Preparation of Arabinogalactooligosaccharides:

[0059] (1) Degradation: Take 0.8g arabinogalactan in a 100ml hydrolysis bottle, add 40mL of 0.05mol / L citric acid buffer in the hydrolysis bottle, add β- 1,3-galactosidase (that is, add 30Uβ-1,3-galactosidase per gram of arabinogalactan), add distilled water to make the total volume of enzymolysis 50mL, adjust the pH value to 5.5, and 200 rev / min, 45 ℃ of constant temperature shaker in enzymolysis 48h, centrifugation removes precipitation, obtains supernatant; Continue to add α-arabinosidase (namely Add 40U α-arabinosidase to each gram of arabinogalactan), add distilled water to make the total volume of enzymolysis 50mL, adjust the pH value to 6.5, enzymolyze in a constant temperature shaker at 200 rpm and 50°C for 50h, centrifuge Remove the precipitate, and finally obtain the degraded supernatant, that is, the degradation solution.

[0060] (2) Membrane separation and purification: Dilute the degradation solution obtai...

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Abstract

The invention discloses arabinogalacto-oligosaccharide and preparation and application thereof. The arabinogalacto-oligosaccharide is composed of polysaccharide with a weight percentage content of 99%or above, the polysaccharide is composed of arabinose and galactose in a molar ratio of 1: 16, wherein the weight-average molecular weight of the arabinogalacto-oligosaccharide is 4,000 to 5,000 Da.According to the preparation, two-step enzymolysis is adopted, and the degraded arabinogalacto-oligosaccharide is separated and purified by adopting an ultrafiltration-nanofiltration coupled mode, sothe arabinogalacto-oligosaccharide with the weight-average molecular weight of 4,000 to 5,000 Da is directionally obtained; and purification time is greatly shortened, and the problem that oligosaccharide with a certain fixed molecular weight is not easy to separate in the prior art is solved. The branched chain substitution degree of the arabinogalacto-oligosaccharide is reduced, the biological functional activity of the arabinogalacto-oligosaccharide is obviously enhanced, and the arabinogalacto-oligosaccharide has an obvious in-vitro growth promoting effect on probiotics such as bifidobacteria, so the arabinogalacto-oligosaccharide can be used as functional oligosaccharide to the fields of food additives, health care products and medicines.

Description

technical field [0001] The invention relates to the technical field of polysaccharides, in particular to an arabinogalactooligosaccharide and its preparation and application. Background technique [0002] Arabinogalactan (Arabinogalactan, AG) is a kind of neutral polysaccharide with highly branched chains, which has a variety of biological activities such as anti-tumor, anti-oxidation, immune regulation, etc. Studies have shown that AG also has the function of regulating the intestinal tract , In 2002, it has been certified and approved by the US FDA as a food additive. The relative molecular mass of AG is about 6.8×10 5 Da, its structure mainly consists of galactan as the main chain, arabinose as its branch chain and galactose through β-1,3 bonds or β-1,6 bonds, but its relative molecular mass is relatively large, so it needs to be Degradation can improve its biological activity. [0003] However, the degradation cannot be oriented to obtain oligosaccharides with the des...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12P19/04C12P19/14
CPCC08B37/006C12P19/04C12P19/14
Inventor 贺亮施锴云程俊文王衍彬王进魏海龙胡传久李海波
Owner ZHEJIANG FORESTRY ACAD
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