Supercharge Your Innovation With Domain-Expert AI Agents!

Method for detecting dimehypo in tea leaves

A detection method and a technique for dimethoside are applied in the field of detecting dimethoate in tea, and can solve the problems of poor stability and low sensitivity, etc.

Inactive Publication Date: 2013-12-25
ZUNYI INST OF PROD QUALITY INSPECTION & TESTING
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is that the method for analyzing dimehypo in the prior art has certain characteristics such as low sensitivity or poor stability, and the present invention provides a high sensitivity, high stability, and easy-to-operate analysis method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting dimehypo in tea leaves
  • Method for detecting dimehypo in tea leaves
  • Method for detecting dimehypo in tea leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1, standard curve

[0013] Nereistoxin standard solution: 100ug / mL, take the standard solution and dilute it with chromatographic grade methanol to prepare 0.05, 0.1, 0.2, 0.4, 1.0ug / mL standard series.

[0014] Instrument used: Brukerscion436-GC, GC-MS chromatographic conditions are: ion source: EI; column type: BR-1ms; inlet temperature: 280°C; column flow rate: 1.0mL / min; Keep at 60°C for 2 minutes, raise to 180°C at a rate of 20°C / min, then rise to 260°C at a rate of 10°C / min, then rise to 280°C at a rate of 30°C / min and hold for 10 minutes; Quantitative ion: 70.0; Qualifier ions: 71.2, 149.1, 56.3; transfer line temperature: 270°C, EI source temperature: 220°C; injection volume: 1ul.

[0015] figure 1 Be the standard curve figure of the dimehypo measured by the present invention, the ordinate in this graph represents the response value of the gas chromatography peak area, and the abscissa represents the concentration of dimehypo; figure 1 It can be se...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting dimehypo in tea leaves. The detecting method comprises the following steps of weighing a testing sample, adding a hydrochloric acid solution, carrying out ultrasonic treatment, filtering, washing residues by the hydrochloric acid solution, adjusting the pH value of the solution to 8.5-9.0 by a sodium hydroxide solution, adding a sodium sulphide solution, reacting in water bath at 70 DEG C for 2.5 hours, cooling the reaction product taken out of the water bath, adding trichloromethane for extracting, layering, extracting repeatedly twice, combining extracted liquids, drying by anhydrous sodium sulfate, carrying out spin drying, making up to volume of 1.0mL by methyl alcohol, and performing GC-MS (gas chromatography-mass spectrometer) quantitative analysis, thereby obtaining the content of dimehypo in tea leaves. The detecting method provided by the invention has the advantages of high sensitivity, good stability, simplicity in operation and the like.

Description

technical field [0001] The invention belongs to the field of analysis of pesticide residues in tea, in particular to a method for detecting dimehypo in tea. Background technique [0002] Dimehypo belongs to the class of nereistoxins and is a broad-spectrum insecticide. Dimehytap is widely used in rice, leaf vegetables and other crops to control rice stem borers, leaf rollers, rice bracts, thrips, leafhoppers, rice planthoppers, cabbage caterpillars, diamondback moths, etc., and is moderately toxic to humans and livestock . [0003] At present, the detection of dimehypo content includes non-aqueous titration method (arbitration method), thin layer-bromination method, high performance liquid chromatography, gas chromatography and gas chromatography-mass spectrometry. Among them, GB5009.114 "Determination of Dimehypo Residues in Rice" uses gas chromatography-flame photometry (GC-FPD) detection, but the sensitivity of this method is not high, and it is greatly disturbed by the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02
Inventor 王兰兰向丽萍李霞马昌红任道援朱丽李梅
Owner ZUNYI INST OF PROD QUALITY INSPECTION & TESTING
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com