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Graft materials derived from mammalian cartilage

A mammalian and cartilage technology, applied in animal cells, vertebrate cells, microorganisms, etc., can solve problems such as high cost, difficult to handle, and the spread of pathogens

Active Publication Date: 2015-07-22
韩士生科(株)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, since it uses human cartilage, it has some disadvantages such as being difficult to handle in large quantities, always having the risk of spreading pathogens due to being an allograft, and high cost of manufacturing the final product for transplantation

Method used

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  • Graft materials derived from mammalian cartilage
  • Graft materials derived from mammalian cartilage
  • Graft materials derived from mammalian cartilage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Determination of the optimal dose of embodiment 1.γ-ray

[0057] Fresh cartilage extracted from pig ears was soaked in 9% NaCl solution for 5 h and irradiated with ultrasound. Chondrocytes are removed by this treatment. Subsequently, it was immersed in 1% hydrogen peroxide as a virus inactivating solution for 45 minutes and stirred. Viruses and other pathogens are inactivated by this treatment. Then, it was immersed in 0.5M sodium hydroxide (NaOH) as an alkaline solution for 30 minutes. Before each treatment, the pre-treatment solution was thoroughly washed out with the washing solution. Dehydration was performed by sequential immersion in 50%, 80%, and 100% acetone to minimize damage to cartilage tissue, followed by sealing in containers and gamma-irradiation. The cartilage graft prepared by the above method was gamma-irradiated in units of 5 kGy in the range of 5 kGy to 30 kGy in order to determine the optimal dose of gamma rays. The optimal dose of γ-rays was ...

Embodiment 2

[0063] Example 2. Comparison of dehydration

[0064] The prepared cartilage was dehydrated by treatment with acetone or glycerol. Glycerol treatment is performed by soaking the tissue in glycerol including about 0.11% antibiotic and then soaking in greater than 99.9% glycerol to replace water in the tissue with glycerol.

[0065] refer to Figure 9 , comparing the thermal properties of the final product after dehydration by using glycerol. According to the change of thermal properties with dehydration shown in the figure Figure 9 , the glass transition temperature of acetone is about 99°, and that of glycerol is about 96°. Therefore, no significant difference was noted, and the thermal properties of acetone and glycerol did not affect the shape of the specimen. According to the change of cytotoxicity with dehydration according to the graph Figure 10 , also noted the absence of cytotoxicity using acetone or glycerol for dehydration.

[0066] Therefore, the cartilage g...

Embodiment 3

[0068] The mensuration of the ECM factor of embodiment 3. technology

[0069] Such as Figure 5 and Figure 6 The amounts of keratan sulfate, collagen II, fibronectin, and chondroitin sulfate in fresh porcine ear cartilage and final cartilage grafts were determined as indicated in . Keratan sulfate was observed everywhere in the stroma, while chondroitin sulfate was mainly observed near the perichondrium, and chondroitin sulfate was especially concentrated on one side of the perichondrium. Note that the amount of Keratan Sulfate, Collagen II, Fibronectin and Chondroitin Sulfate in the final product decreased slightly, but remained and maintained.

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Abstract

The present invention relates to a graft material derived from mammalian cartilage. The graft material according to the present invention has significantly lowered risk by eliminating antigenicity, pathogen and cytotoxicity through multi-step processing treatments and inactivating all kinds of pathogens without affecting biomechanics and tissue reconstruction performance. It allows smooth accepting and supplying and low cost manufacturing because of use of mammalian cartilage not human's.

Description

technical field [0001] The present invention relates to graft materials derived from mammalian cartilage. Background technique [0002] It has been widely used in the field of orthopedic surgery to treat damaged cartilage or transplant cartilage. Rhinoplasty has been increasing in response to greater demand especially for rhinoplasty. A variety of grafts have been used for decades to do this, including autologous cartilage grafts. Among them, allografts, such as gore-tex or silicon, have the advantages of easy handling, saving operation time and eliminating donor site morbidity. Related prior art using heterogeneous materials are Korean Patent Application Publication No. 2011-0012807, which discloses artificial cartilage using balloon and shape memory alloy; and Korean Patent Application Publication No. 2011-0097662, which discloses artificial cartilage for Regenerated scaffolds and methods for their preparation. [0003] However, side effects such as foreign body sensat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61F2/30A61F2/28C12N5/077C12N13/00
CPCA61L27/3691A61L27/3817A61L2430/02A61L2430/40C12N5/0655C12N13/00
Inventor 黄镐灿姜启远金镇荣安宰亨
Owner 韩士生科(株)有限公司
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