Extracting method and application of seabuckthorn flavone

A technology of seabuckthorn flavonoids and an extraction method, which is applied in the field of extraction of seabuckthorn flavonoids, can solve the problems of large dosage of patients, rough process, many impurities and the like

Inactive Publication Date: 2014-01-08
NANJING ZHENGLIANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the prior art, there is no report on the joint application of seabuckthorn flavonoids using supercritical and microwave technology in the extraction and preparation. However, the current extraction method is rough and backward in process and has many impurities, which leads to excessive dosage for patients, which is inconvenient to take and seriously affects This product has been applied clinically

Method used

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  • Extracting method and application of seabuckthorn flavone
  • Extracting method and application of seabuckthorn flavone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 1000g of seabuckthorn and add it to CO 2 In a supercritical extractor, ethanol is used as an entrainer, and the entrainer accounts for 4% by volume of the total extraction solvent. The extraction pressure is 15MPa, the temperature is 30°C, and the CO 2 The flow rate is 1m1 / g crude drug·min, the extraction time is 150min, and the supercritical extract is obtained, then 70% ethanol is added, and put into the microwave extraction device for microwave extraction, the extraction power is 400W, and the extraction is performed twice, each time for 4 minutes, and each time the added The amount of 70% ethanol is 2000g, the extract is obtained, concentrated, added to a D101 macroporous adsorption resin column, eluted with 50% ethanol, collected 5 times the column volume eluate, recovered ethanol under reduced pressure, concentrated and dried to obtain seabuckthorn Flavonoid extract, the content of isorhamnetin determined by high performance liquid chromatography is 53.6%.

Embodiment 2

[0021] Take 1000g of seabuckthorn and add it to CO 2 In a supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer in the total extraction solvent is 6%, the extraction pressure is 30MPa, the temperature is 50°C, and the CO 2 The flow rate is 3m1 / g crude drug·min, the extraction time is 180min, and the supercritical extract is obtained, then 70% ethanol is added, and put into a microwave extraction device for microwave extraction, the extraction power is 600W, and the extraction is performed twice, each time for 8 minutes, and each time the added The amount of 70% ethanol is 2000g, the extract is obtained, concentrated, added to a D101 macroporous adsorption resin column, eluted with 50% ethanol, collected 5 times the column volume eluate, recovered ethanol under reduced pressure, concentrated and dried to obtain seabuckthorn Flavonoid extract, the content of isorhamnetin determined by high performance liquid chromatography is 54.3%....

Embodiment 3

[0023] Take 1000g of seabuckthorn and add it to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the entrainer accounts for 5% by volume of the total extraction solvent, the extraction pressure is 20MPa, the temperature is 40°C, and the CO 2 The flow rate is 2m1 / g crude drug·min, the extraction time is 160min, and the supercritical extract is obtained, then 70% ethanol is added, and put into the microwave extraction device for microwave extraction, the extraction power is 500W, and the extraction is performed twice, each time for 6 minutes, and each time the added The amount of 70% ethanol is 2000g, the extract is obtained, concentrated, added to a D101 macroporous adsorption resin column, eluted with 50% ethanol, collected 5 times the column volume eluate, recovered ethanol under reduced pressure, concentrated and dried to obtain seabuckthorn Flavonoid extract, the content of isorhamnetin determined by high performance liquid chromatography is 55.2%. ...

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Abstract

The invention provides an extracting method of seabuckthorn flavone which is prepared by adopting supercritical extraction and microwave extraction, so that content is greatly improved. The invention further provides application of the seabuckthorn flavone in preparation of a drug for restraining cell proliferation of C57BL / 6 mouse fibrosarcoma cells S180 and application of the seabuckthorn flavone in preparation of a drug for increasing bone density.

Description

technical field [0001] The invention relates to the technical field of extraction of traditional Chinese medicines, in particular to an extraction method and application of seabuckthorn flavonoids. Background technique [0002] The plant seabuckthorn is a deciduous shrub belonging to the genus Hippophaeaceae. It is characterized by drought resistance, wind and sand resistance, and can survive on saline-alkali land, so it is widely used in water and soil conservation. Domestically distributed in North China, Northwest, Southwest and other places. Seabuckthorn is a medicinal and edible plant. The roots, stems, leaves, flowers, and fruits of seabuckthorn, especially the fruit of seabuckthorn, are rich in nutrients and biologically active substances, and can be widely used in many fields of the national economy such as food, medicine, light industry, aerospace, agriculture, animal husbandry and fishery. Seabuckthorn fruit used as medicine has the effects of relieving cough and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P35/00A61P19/08A61P19/10
Inventor 不公告发明人
Owner NANJING ZHENGLIANG MEDICAL TECH
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