New pure white hypsizigus marmoreus strain
A technology of white shimeji mushrooms and strains, applied in fungal products, botanical equipment and methods, lichen products, etc., can solve the problems of reducing the market competitiveness of products, long cultivation and cultivation cycles, and easy occurrence of nodular mushrooms, etc. Conducive to mechanized and automated operations, short cultivation and cultivation cycles, and suitable for large-scale promotion of planting
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Embodiment 1
[0032] Embodiment 1 hybridization obtains Finc-W-247
[0033] This example illustrates the breeding process of the pure white mushroom strain Finc-W-247 of the present invention. Specific steps are as follows:
[0034] 1) Select parent strains: select TNN-11 and H-W as parents, the fruiting bodies of these two parents can be purchased in the market, and are also preserved in the strain collection center of Shanghai Academy of Agricultural Sciences.
[0035] 2) Preparation of culture medium
[0036] PDA medium: use 200g of potatoes, 20g of glucose and 20g of agar per 1L of medium.
[0037] Original seed culture medium: 65% of sawdust (percentage by weight, the same below), 18% of rice bran, 7% of bran, 10% of corn flour, and the water content is 60% to 62%.
[0038] Cultivated medium: 40% corn cob, 24% soybean hulls, 14% rice bran, 17% bran, 5% corn flour, and the water content is 64% to 66%.
[0039] 3) Obtain 800 heterozygotes by conventional hybridization method.
[004...
Embodiment 2
[0043] Embodiment 2 bacterial age test
[0044] The present embodiment compares the culture period (i.e. bacterial age), cultivation period and per unit yield of the pure white shimeji mushroom strain Finc-W-247 of the present invention with the most widely planted H-W in the existing pure white shimeji mushroom varieties, and concludes The advantages and characteristics of the pure white jiji mushroom strain Finc-W-247 of the present invention.
[0045] The cultivar culture medium that present embodiment adopts is:
[0046] Medium A:
[0047] 65% sawdust, 18% rice bran, 7% bran, 10% corn flour, among which the water content is 60-62%.
[0048] Medium B is:
[0049]25% corn cob, 23% miscellaneous sawdust, 20% soybean hulls, 12% rice bran, 15% bran, 5% corn flour, of which the water content is 64% to 66%.
[0050] Taking H-W as a control, set 9 bacterial ages from 70d (ie days, the same below) to 110d for culture time, every 5d is a bacterial age, and each bacterial age is ...
Embodiment 3
[0054] Embodiment 3 preservation test
[0055] After Finc-W-247 and H-W are harvested, they are packed in 155-165g packs, 24 packs each, stored in cold storage (3°C-8°C) for 11 days, and then taken out and placed in an environment of 23°C to record the quality changes of fruiting bodies Condition. Among them, normal: it means that the fruiting body is completely fresh; starting to deteriorate: it means that one or more fruiting bodies have started to rot, but the whole still has commodity value; completely deteriorated: more than two fruiting bodies are moldy and have no commercial value.
[0056] The test results are shown in Table 2. After being stored in a cold storage (3°C-8°C) for 11 days, they were taken out and placed in an indoor environment at 23°C. Finc-W-247 was still intact after being stored for 13 days. 10d began to turn from snow white to yellow, and lost its freshness, while the 18d part of Finc-W-247 began to turn yellow; after H-W was stored in cold storage ...
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