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Silkworm chrysalis polypeptide skin rejuvenation mask and preparation method thereof

A technology of silkworm chrysalis protein polypeptide and silkworm chrysalis, which is applied in the field of cosmetics and skin care products, which can solve the problems of high degree of heat denaturation of silkworm chrysalis protein, allergies of users, and difficulty in absorption, etc., to achieve abundant sources, reduce environmental pollution and waste of resources, and low price Effect

Active Publication Date: 2015-08-19
INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1. The silk reeling process generally requires high temperature treatment, so silkworm chrysalis protein has a high degree of thermal denaturation and poor solubility. If it is directly used in the preparation of cosmetics, it is not easy to be absorbed by the skin; Substances absorbed by the skin;
[0007] 2. Silkworm chrysalis protein is a substance with high protein content. Since some people are allergic to high protein, if the protein ingredients contained in it are directly used in the cosmetics industry, it will also cause allergies to some users

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Step 1: Two-step enzymatic hydrolysis to prepare silkworm chrysalis protein polypeptide

[0037] (1) Prepare a silkworm chrysalis protein solution with a concentration of 12% with deionized water, add papain, the enzyme amount is 20000IU / g silkworm chrysalis protein, the enzymatic hydrolysis temperature is 55°C, the pH value is 7.0, the enzymatic hydrolysis time is 3h, and the enzymatic hydrolysis is completed at 92°C Inactivate the enzyme for 13 minutes;

[0038] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 9.0, add Esperase protease, the amount of enzyme added is 0.15KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 3 hours, the enzymatic hydrolysis temperature is 55°C, and the enzyme is extinguished at 92°C after the enzymatic hydrolysis is completed. Enzyme 13min;

[0039] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, cle...

Embodiment 2

[0065] Step 1: Two-step enzymatic hydrolysis to prepare silkworm chrysalis protein polypeptide

[0066] (1) Prepare a silkworm chrysalis protein solution with a concentration of 10% with deionized water, add papain, the enzyme amount is 8000IU / g silkworm chrysalis protein, the enzymatic hydrolysis temperature is 50°C, the pH value is 7.5, the enzymatic hydrolysis time is 4h, and the enzymatic hydrolysis is completed at 95°C Inactivate the enzyme for 10 minutes;

[0067] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 9.5, add Esperase protease, the amount of enzyme added is 0.075KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 4 hours, the enzymatic hydrolysis temperature is 50°C, and the enzyme is extinguished at 95°C after the enzymatic hydrolysis is completed. Enzyme 10min;

[0068] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, cle...

Embodiment 3

[0076] Step 1: Two-step enzymatic hydrolysis to prepare silkworm chrysalis protein polypeptide

[0077] (1) Prepare silkworm chrysalis protein solution with a concentration of 10% in deionized water, add papain, enzyme amount 60000IU / g silkworm chrysalis protein, 3%, enzymolysis temperature 45℃, pH value 6.5, enzymolysis time 2h, enzymolysis is completed Then inactivate the enzyme at 90°C for 15 minutes;

[0078] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 10.0, add Esperase protease, the amount of enzyme added is 0.225KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 4 hours, the enzymatic hydrolysis temperature is 45°C, and the enzymatic hydrolysis is extinguished at 90°C Enzyme 15min;

[0079] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, clear and transparent solution. The polypeptide solution is passed through an ultra-microfi...

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Abstract

The invention relates to the technical field of cosmetics and skin care products, in particular to a silkworm chrysalis polypeptide skin rejuvenation mask: 5-10 parts of silkworm chrysalis protein polypeptide, 1-2 parts of rosemary essential oil, 1-3 parts of pomegranate seed extract, 1 part of seaweed extract ~6 parts, 3~6 parts of glycerin, 1~4 parts of olive oil, 2~5 parts of caprylic triglyceride, 1~3 parts of pearl powder. The silkworm chrysalis protein polypeptide is obtained by hydrolyzing silkworm chrysalis protein with papain and Esperase protease. Active silkworm chrysalis polypeptide combined with active substances such as rosemary extract and pomegranate seed extract can help the skin resist free radicals and collagenase damage, thereby effectively preventing and resisting wrinkles, increasing skin elasticity, and delaying aging. The facial mask prepared by using silkworm chrysalis protein and plant extracts as raw materials is natural, mild and non-irritating to the skin, and multiple active substances act synergistically on the skin, and the effect is better than that of ordinary facial masks.

Description

technical field [0001] The invention relates to the technical field of cosmetics and skin care products, in particular to a silkworm chrysalis polypeptide skin rejuvenating mask, and also relates to a preparation method of the silkworm chrysalis polypeptide skin rejuvenating mask. Background technique [0002] silkworm chrysalis( silkworm pupa ), the chrysalis of Bombyx mori, the silkworm moth, is the main by-product of the silk industry. my country is a big country in the silk industry. There are hundreds of thousands of tons of leftovers from silk reeling factories every year. Silkworm chrysalis resources are extremely rich. However, due to the lack of deep processing technology, most silkworm chrysalis are used as feed or fertilizer, or even discarded directly. The resource utilization rate and The added value is low, resulting in great waste. [0003] Silkworm chrysalis contains a variety of active substances, among which the protein content of silkworm chrysalis is ab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/98A61K8/97A61K8/92A61K8/64A61Q19/00A61Q19/08
Inventor 刘玮杜方岭陈相艳刘丽娜徐同成陶海腾邱斌
Owner INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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