Oral vaccine strain for recombined rabies virus and preparation method thereof
A technology of rabies virus and oral vaccine, which is applied in the field of recombinant rabies virus oral vaccine strain and its preparation, can solve problems such as infection, and achieve the effect of enhancing immunogenicity and good replicability
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[0025] Example 1 Design, rescue, identification and preliminary evaluation of immune efficacy of recombinant virus LBNSE-dog GM-CSF
[0026] 1 Materials and methods
[0027] 1.1 Material
[0028] 1.1.1 Viruses
[0029] The LBNSE strain is provided by the State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University. For specific information and construction methods of the virus, please refer to the relevant literature (Wen Y, Wang H, Wu H, Yang F, Tripp RA, et al. (2011) Rabies virus expressing dendritic cell-activating molecules enhances the innate and adaptive immune response to vaccination. J Virol85:1634-1.).
[0030] 1.1.2 Plasmid
[0031] For the construction strategy and specific methods of the infectious clone pLBNSE used to rescue the LBNSE strain, please refer to the relevant literature (Wen Y, Wang H, Wu H, Yang F, Tripp RA, et al. (2011) Rabies virus expressing dendritic cell-activating molecules enhances the innate and adaptive immune response to vacc...
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