Multiple PCR method for detecting quinolone antibiotics campylobacter jejuni and kit
A technology of Campylobacter jejuni and quinolones, applied in the biological field, can solve the problems of long time consumption and poor sensitivity, and achieve the effects of fast and accurate detection, good sensitivity and simple detection technology
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[0030] Example 1. Integrating mismatch amplification mutation analysis PCR (MAMA-PCR) and multiplex PCR technology for strain identification rapid detection of quinolone-resistant Campylobacter jejuni
[0031] Strains to be tested:
[0032] Salmonella resistant to quinolone antibiotics (No. 395, this laboratory’s own strain collected from China, confirmed by drug susceptibility tests that it is resistant to quinolone antibiotics), Salmonella ATCC13311 (purchased from the American Standard Biological Products Collection), Campylobacter coli (numbered TA-60, this laboratory's own strain collected from China, confirmed by drug susceptibility tests to be quinolone antibiotic-resistant Campylobacter coli), quinolone antibiotic-resistant Campylobacter jejuni DH69, quinolone drugs The sensitive Campylobacter jejuni model strain 11168 (numbered NCTC11168), and the quinolone-sensitive (non-resistant) Campylobacter jejuni standard quality control strain 33560 (numbered ATCC33560).
[0033] Qu...
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