Production method of porcine circovirus type 2

Abstract

The invention discloses a production process for producing porcine circovirus type 2 by culturing cells pK-15A1# in a bioreactor. The production process comprises the technical steps of (1) selecting pK-15A1# cells for multiplying circovirus type 2; (2) utilizing the bioreactor as a cell culture tool; (3) carrying out a virus culture process of synchronous virus inoculation; (4) culturing the pK-15A1# cells on micro-carriers in the bioreactor; (5) carrying out multiplication culture on viruses of porcine circovirus type 2 in the cells on the micro-carriers in the bioreactor; and (6) harvesting virus culturing materials of porcine circovirus type 2. The production process has the advantages that the production cost can be lowered, and compared with that of a traditional spinner bottle production process, the input-output ratio of the production process is increased by 5-10 times; the production cycle is short, the occupied area is small, the production scale can be easily and rapidly expanded, the environmental pollution is slight and is easy to avoid, the automation degree is high, few workers are required, the quality is stable, the cost can be remarkably lowered, and the yield and quality of vaccines can be remarkably improved.

Description

technical field [0001] The invention relates to a vaccine production method using bioreactor cell culture technology, which can be used for industrial production of porcine circovirus type 2 vaccine and replaces the traditional production process of rotary bottle culture method. Background technique [0002] At present, the domestic production of porcine circovirus type 2 inactivated vaccines is mainly realized by the method of cell spinner bottle culture. This traditional process produces semi-finished products with low antigen titers, and it is difficult to meet the requirements for vaccine matching. It requires labor-intensive, time-consuming and low-efficiency. , high production costs; easy to be polluted by the environment; large differences between different production batches or between different bottles of the same production batch; difficult to expand production; prone to hidden dangers in vaccine quality caused by bacteria or other virus contamination, involving bio...

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Problems solved by technology

[0002] At present, the domestic production of porcine circovirus type 2 inactivated vaccines is mainly realized by the method of cell transfer bottle culture. This traditional process produces semi-finished products with low antigen titers, and it is difficult to meet the requirements for vaccine matching. It requires labor-intensive, time-consuming and low-efficiency. , high production costs; easy to be polluted by the environment; large differences between different production batches or between different bottles of the same production batch; difficult to expand production; prone to hidden dangers of vaccine quality caused by bacteria or other virus contamination, involving biological safety and public health Hygiene issue

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