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Beauveria bassiana strain capable of killing chrysomelidae insects and application thereof

A technology of Beauveria bassiana and strains, applied in application, pesticides, biocides, etc., can solve problems such as polluted environment, inability to achieve effective control of pest populations, and rampant pests.

Inactive Publication Date: 2014-02-05
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Leaf beetle pests are more sensitive to organophosphorus, organochlorine, and permethrin pesticides. Although these drugs can achieve short-term effects, they also bring some disadvantages, such as enhancing pest resistance, polluting the environment, killing birds, causing pests The vicious cycle of rampant again cannot achieve effective control of the pest population

Method used

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  • Beauveria bassiana strain capable of killing chrysomelidae insects and application thereof
  • Beauveria bassiana strain capable of killing chrysomelidae insects and application thereof
  • Beauveria bassiana strain capable of killing chrysomelidae insects and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Workflow of XWY-1 strain acquisition

[0015] Collect naturally susceptible dead worms in the field→isolate and purify the strains→inoculate Ulmus indigo beetle larvae to determine the insecticidal virulence→obtain the highly virulent strain XWY-1→strain morphology, spore morphology, identification→extract DNA of the strain and use the ITS sequence of the fungus Universal primers were used to amplify the strain DNA, and the obtained amplified sequence was sequenced and compared in GenBank → the strain XWY-1 was determined to be Beauveria bassiana.

Embodiment 2

[0016] Embodiment 2: the screening and isolation process of XWY-1 bacterial strain

[0017] The XWY-1 strain was isolated from white susceptible insects collected by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences in 2010 during the survey of sweet potato fields in Xiong County, Hebei Province. The white susceptible insects were brought back to the laboratory and treated with moisture. When spores were produced on the body surface, the spores were inoculated in SDAY (10 g peptone, 10 g yeast, 40 g glucose, 15 g agar, 1000 mL distilled water, natural pH ) on plate medium until conidia were produced, collected and freeze-dried at 4°C for later use.

Embodiment 3

[0018] Embodiment 3: Bacterial strain culture and spore suspension preparation

[0019] The XWY-1 strain was inoculated on the SDAY plate medium and cultivated under constant temperature and light (14L:10D) at 25±1°C. After the formation of conidia, the spores were collected with 0.05% (v / v) sterile Tween 80 aqueous solution, stirred with a magnetic stirrer for 30 min, the spores were completely and evenly dispersed, and then filtered through 4 layers of sterile gauze to obtain a pure spore suspension , counted with a hemocytometer and prepared into a gradient concentration of spore suspension for the determination of pathogenicity.

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PUM

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Abstract

The invention discloses a new beauveria bassiana strain XWY-1 which has high poisoning capability on forestry important insects and especially on coleoptera chrysomelidae insect pyrrhalta aenescens. Toxicity tests show that XWY-1 strain has extremely toxicity on coleoptera chrysomelidae insect pyrrhalta aenescens, and therefore beauveria bassiana XWY-1 can be prepared into pesticides for controlling forestry insects, and thus the use amount of pesticides is reduced, the environmental pollution is reduced, natural enemies are protected, and beauveria bassiana XWY-1 has important economical value and application prospect.

Description

technical field [0001] The invention relates to a new microbial strain and its application, in particular to a Beauveria bassiana and its application in forestry pest control. Background technique [0002] Beauveria bassiana ( Beauveria bassiana ) is a pathogenic fungus of a class of parasitic insects. It adheres to the epidermis or stomach of the insects, reproduces rapidly, causes the death of the pests, and causes the epidemic infection of the pests with conidia. Beauveria bassiana is a fungal insecticide with the longest research time and a large application area in my country. Since the 1960s, China began to study the use of Beauveria bassiana to control the forestry pest pine caterpillar and the agricultural pest corn borer. In the 1970s, solid-phase culture and liquid-solid two-phase production methods and application technologies were developed, and factories were successively built. Produce crude products of Beauveria bassiana, and control pine caterpillars and co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 杜立新曹伟平宋健冯书亮王金耀
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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