Method for preparing 7-ACA (aminocephalosporanic acid) and obtaining alpha-aminoadipic acid by one-step enzymatic reaction

Abstract

The invention relates to a method for preparing 7-ACA (aminocephalosporanic acid) and obtaining alpha-aminoadipic acid. The method comprises the following steps of (1) treating cephalosporin C fermentation liquid; (2) performing an enzymolysis reaction of cephalosporin C under the effect of cephalosporin C acyltransferase to obtain 7-ACA; (3) treating the 7-ACA lysate after the enzymolysis; (4) separating out the 7-ACA; (5) separating alpha-aminoadipic acid from the separated 7-ACA lysate.

Description

Technical field: [0001] The invention relates to the application of an enzymatic cleavage method, in particular to a one-step enzymatic application for preparing 7-aminocephalosporanic acid and obtaining alpha-aminoadipic acid. Background technique: [0002] 7-aminocephalosporanic acid (7-aminocephalosporanic acid, 7-ACA), [0003] Structural formula: [0004] It is an important basic raw material for semi-synthetic cephalosporins. At present, cephalosporin C (Cephalosporin C, CPC) is usually cleaved by chemical method or two-step enzymatic method in industry to remove the 7-position D-α-aminohexadiene Acyl side chains give 7-ACA. [0005] α-Aminoadipic acid [0006] α-Aminoadipic acid (α-Aminoadipic acid), molecular weight 161.16, molecular formula C6H11N1O4, white crystal or white crystalline powder. It was first found in corn seeds or in the urine of humans and guinea pigs. Belonging to a kind of amino acid, it is widely used in medicine, food and feed, cosmetics i...

AI Technical Summary

Problems solved by technology

Although the two-step enzymatic method solves the environmental pollution problem of the chemical method, the two-step enzymatic method is somewhat insufficient in terms of yield, purity and cost, and the process of the previous step GL-7-ACA is difficult to control

[0011] Although these methods are superior to the two-step enzymatic method for preparing 7-aminocephalosporanic acid by one-step enzymatic method, the efficiency of different enzymes is very different, and its specific treatment method will also have an impact on the yield and purity of the product. , and does not involve the recovery and treatment of α-aminoadipic acid, the present invention finds a method that combines the chemical method (high yield and high purity) and the two-step enzymatic method ( The advantages of environmental protection and high yield), and at the same time, a simple and efficient method for recovering α-aminoadipic acid is provided, which is simpler than the 7-ACA obtained by the existing one-step enzymatic method, and the product yield is higher and the purity is higher High, the reaction conditions are milder and faster, and the discharged wastewater has almost no pollution, which not only saves production costs, protects the environment, but also creates new benefits

Images