Unlock instant, AI-driven research and patent intelligence for your innovation.
A method for cultivating strong seedlings of potato virus-free test-tube plantlets
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A potato and test-tube seedling technology, which is applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve the problems of being unsuitable for large-scale seedling raising, affecting the inoculation of virus-free seedlings, and the excessive growth of test-tube seedlings, so as to improve the transplanting efficiency. Effects of survival rate, short culture period and short internodes
Inactive Publication Date: 2016-04-13
四川省植物工程研究院
View PDF3 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
The production of virus-free potato seedlings using traditional medium is prone to excessive growth of test-tube seedlings, small stem diameter, long internodes, small leaves, "burnt tips" and many fibrous roots, which not only affect the virus-free seedlings. The working efficiency of inoculation and transplanting reduces the reproduction coefficient and transplanting survival rate of virus-free seedlings. Therefore, it is particularly important to conduct research on promoting the growth of virus-free test-tube seedlings of potato, so as to cultivate robust and high-quality virus-free test-tube seedlings.
[0003] Chinese patent application CN200710066315.6 discloses a method for floating seedlings of potato detoxification tube tubers, which improves the emergence rate of tube tubers, but needs to build seedling ponds, the cost is high, it is not suitable for large-scale seedling cultivation, and the operation is inconvenient
Chinese patent application CN201210450346.2 discloses a cycle production process of virus-free potato test-tube tubers, including the following steps: test-tube plantlets strong seedling cultivation, test-tube tuber induction, after the test-tube plantlets strong seedlings training step, the test-tube plantlets strong seedlings are cultivated After cutting the terminal buds of the virus-free potato plantlets, add the virus-free potato plantlets without the terminal buds to the test-tube tube induction medium to induce the production of test-tube tubers; the clipped terminal buds continue to be cultivated for strong seedlings of the test-tube plantlets. The formula of solid strong seedling medium in this process is MS+10mg L -1 B9+3.5g / L agarpowder+30g / L sugar, the formula in the induction medium is MS+8mg·L -1 BA+100g / L sugar, KNO in MS medium 3 , NH 4 NO 3 , KH 2 PO 4 ,MgSO 4 ,CaCl 2 The concentration is higher, the cost is higher, the process cycle is long, and the liquid strong seedlings are cultivated for about 25 days
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0018] Example 1 Rapid Propagation and Transplanting of Virus-free Potato Seedlings
[0019] (1) Preparation of culture medium:
[0020] Prepared to contain 950mg·L -1 KNO 3 , 825mg·L -1 NH 4 NO 3 , 100mg·L -1 K H 2 PO 4 ,185mg·L -1 MgSO 4 ,220mg·L -1 CaCl 2 , 27.8mg·L -1 FeSO 4 ·7H 2 O, 37.3mg·L -1 Na 2 ·EDTA·2H 2 O, 0.83mg·L -1 KI, 6.2mg·L -1 h 3 BO 3 , 22.3mg·L -1 MnSO 4 4H 2 O, 8.6mg·L -1 ZnSO 4 ·7H 2 O, 0.25 mg·L -1 Na 2 MO 4 2H 2 O, 0.025mg·L -1 CuSO 4 ·5H 2 O, 0.025mg·L -1 CoCl 2 2H 2 O, 100mg·L -1 Inositol, 2mg·L -1 Glycine, 0.1mg·L -1 Thiaminehydrochloride, 0.5mg·L -1 Pyridoxinehydrochloride, 0.5mg·L -1 Niacin, 30g / L edible sugar, 5.4g / L agar, 0~25mg·L -1 For B9 medium, adjust the pH to 5.8;
[0021] (2) Rapid propagation of virus-free seedlings:
[0022]According to each stem node, an explant is forwardly inserted in the medium in step (1), at 23 DEG C, cultivated for 15 days under 2000lx light conditions, and counted the...
Embodiment 2
[0030] Example 2 Rapid Propagation and Transplanting of Virus-free Potato "Mira" Seedlings
[0031] (1) Preparation of culture medium:
[0032] Prepared to contain 950mg·L -1 KNO 3 , 825mg·L -1 NH 4 NO 3 , 100mg·L -1 K H 2 PO 4 ,185mg·L -1 MgSO 4 ,220mg·L -1 CaCl 2 , 27.8mg·L -1 FeSO 4 ·7H 2 O, 37.3mg·L -1 Na 2 ·EDTA·2H 2 O, 0.83mg·L -1 KI, 6.2mg·L -1 h 3 BO 3 , 22.3mg·L -1 MnSO 4 4H 2 O, 8.6mg·L -1 ZnSO 4 ·7H 2 O, 0.25 mg·L -1 Na 2 MO 4 2H 2 O, 0.025mg·L -1 CuSO 4 ·5H 2 O, 0.025mg·L -1 CoCl 2 2H 2 O, 100mg·L -1 Inositol, 2mg·L -1 Glycine, 0.1mg·L -1 Thiaminehydrochloride, 0.5mg·L -1 Pyridoxine hydrochloride, 0.5mg·L -1 Niacin, 30g / L edible sugar, 5.4g / L agar, 0~25mg·L -1 For B9 medium, adjust the pH to 5.8;
[0033] (2) Rapid propagation of virus-free seedlings:
[0034] According to each stem node, an explant is forwardly inserted in the medium in step (1), at 23 DEG C, cultivated for 15 days under 2000lx light conditions, and cou...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention relates to the technology of strengthening seedlings of potato virus-free tube seedlings, and particularly relates to a method for culturing a strong seedling of a potato virus-free tube seedling. The method mainly comprises the following steps: preparation of a culture medium; rapid propagation of a virus-free seedling; transplanting of the virus-free seedling. According to the method, B9 with different concentrations are added in different stages of tissue culture and rapid propagation of potato by taking an improved 1 / 2 MS as a culture medium and tap water and edible white sugar as raw materials, so that the subculture period is shortened and the test-tube seedling is more robust. The concentration of the B9 added in the rapid propagation stage of the virus-free tube seedling is 4-8mg / L, so that stem diameter, number of effective stems and number of roots of the virus-free tube seedling are effectively promoted. The blade is thickened and deeply green. The subculture period is shortened from conventional 3-4 weeks to just 14-18 days, so that the propagation efficiency of the potato virus-free tube seedling is extremely improved. B9 with concentration of 10-15mg / L is added into the culture medium before the virus-free seedling is transplanted, so that the steam of the virus-free tube seedling is thicker and stronger, shorter in internode and higher in degree of lignification of the stem, and the transplantingsurvival rate of the seedling can be improved.
Description
technical field [0001] The invention relates to a technique for growing strong seedlings of potato virus-free test tube seedlings, in particular to a method for cultivating strong seedlings of potato virus-free test-tube seedlings. Background technique [0002] The establishment of potato virus-free and seed potato production system has fundamentally solved the problem of potato seed degradation, and the production and rapid propagation of virus-free test-tube seedlings is one of the most critical links in the whole system. The production of virus-free potato seedlings using traditional medium is prone to excessive growth of test-tube seedlings, small stem diameter, long internodes, small leaves, "burnt tips" and many fibrous roots, which not only affect the virus-free seedlings. The working efficiency of inoculation and transplanting reduces the reproduction coefficient and transplanting survival rate of virus-free seedlings. Therefore, it is particularly important to carry...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More