Preparation method for antibiosis ultraviolet prevention silk fibroin nanofiber membrane

Abstract

The invention relates to a preparation method for a nanofiber membrane which mixed nano materials which have an antibiosis ultraviolet prevention property and silk fibroin. The preparation method for the antibiosis ultraviolet prevention silk fibroin nanofiber membrane comprises performing surface modification on the nano materials which have the antibiosis ultraviolet prevention performance, adding into a prepared silk fibroin solution and mixing and forming into a spinning solution and obtaining the antibiosis ultraviolet prevention silk fibroin nanofiber membrane through the electrostatic spinning technique. The preparation method for the antibiosis ultraviolet prevention silk fibroin nanofiber membrane has the advantages of enabling nanoscale composite fibers obtained through the electrostatic spinning technique to have good biocompatibility as the silk fibroin, meanwhile to have the antibiosis ultraviolet prevention performance as photocatalysis type nano-antibacterial materials, to be applicable to biomedicine field and particularly to be capable of serving as cover materials for killing bacteria and treating infected wound surface and being simple in preparation technology, low in production cost and capable of satisfying production and application requirements.

Description

technical field

[0001] The invention relates to a nanofiber and a preparation method thereof, in particular to a nanofiber film blended with a nanomaterial having antibacterial and anti-ultraviolet properties and silk fibroin and a preparation method thereof. The method belongs to the preparation technology of fibers, and the prepared materials can be widely used in the fields of biomedicine and the like. Background technique

[0002] Silk fibroin is a protein extracted from silk. It has good biocompatibility and can be prepared into various forms of materials such as film, gel, and powder. Due to its unique physical and chemical properties, silk fibroin materials are currently It has been widely studied in the field of biomedical materials, such as immobilized enzyme materials, cell culture substrates, drug release agents, artificial organs, etc. In order to improve the performance of silk fibroin and make it better used in the field of biomaterials, in recent years, schol...

Examples

Embodiment 1

[0019] 1) Dissolve a certain amount of nano-ZnO in 20 ml of PVP-containing aqueous solution to make the molar ratio 1:1, and react for 4 hours under ultrasonic conditions. After the reaction, the solution is centrifuged, washed, and dried to obtain surface-modified nano ZnO.

[0020] 2) Put silkworm cocoons into a sodium carbonate solution with a mass percentage concentration of 0.5% and stir, boil for 60 minutes to remove sericin on the surface of silkworm cocoons, wash with water, and dry at 60 °C to prepare silk fibroin fibers. The dried silk fibroin The mixed solution of fiber and calcium chloride, water and ethanol with a molar ratio of 1:8:2 is dissolved at 75 °C to form a silk fibroin solution, and the ethanol and calcium chloride in the solution are removed by dialysis, and silk is obtained by filtration. fibroin solution, and then freeze-dried to obtain silk fibroin.

[0021] 3) According to the weight ratio of 1:0.1:0.01, water, silk fibroin, and PVP surface-modifie...

Embodiment 2

[0025] 1) A certain amount of nano-TiO 2 , dissolved in 20 ml of aqueous solution containing Triton X-100 so that the molar ratio was 1:5, and reacted for 4 hours under ultrasonic conditions. After the reaction, the solution was centrifuged, washed, and dried to obtain surface-modified nano-TiO 2 .

[0026] 2) Put silkworm cocoons into a sodium carbonate solution with a mass percentage concentration of 0.5% and stir, boil for 60 minutes to remove sericin on the surface of silkworm cocoons, wash with water, and dry at 60 °C to prepare silk fibroin fibers. The dried silk fibroin The mixed solution of fiber and calcium chloride, water and ethanol with a molar ratio of 1:8:2 is dissolved at 75 °C to form a silk fibroin solution, and the ethanol and calcium chloride in the solution are removed by dialysis, and silk is obtained by filtration. fibroin solution, and then freeze-dried to obtain silk fibroin.

[0027] 3) Nano-TiO surface modified with water, silk fibroin and Triton X...

Embodiment 3

[0030] 1) Dissolve a certain amount of nano-ZnO in 20 ml of PVA-containing aqueous solution to make the molar ratio 1:0.5, and react for 4 hours under ultrasonic conditions. After the reaction, the solution is centrifuged, washed, and dried to obtain surface-modified nano ZnO.

[0031] 2) Put silkworm cocoons into a sodium carbonate solution with a mass percentage concentration of 0.5% and stir, boil for 60 minutes to remove sericin on the surface of silkworm cocoons, wash with water, and dry at 60 °C to prepare silk fibroin fibers. The dried silk fibroin The mixed solution of fiber and calcium chloride, water and ethanol with a molar ratio of 1:8:2 is dissolved at 75 °C to form a silk fibroin solution, and the ethanol and calcium chloride in the solution are removed by dialysis, and silk is obtained by filtration. fibroin solution, and then freeze-dried to obtain silk fibroin.

[0032]3) Hexafluoroisopropanol, silk fibroin and PVA surface-modified nano-ZnO were mixed accordi...

technical field

[0001] The invention relates to a nanofiber and a preparation method thereof, in particular to a nanofiber film blended with a nanomaterial having antibacterial and anti-ultraviolet properties and silk fibroin and a preparation method thereof. The method belongs to the preparation technology of fibers, and the prepared materials can be widely used in the fields of biomedicine and the like. Background technique

[0002] Silk fibroin is a protein extracted from silk. It has good biocompatibility and can be prepared into various forms of materials such as film, gel, and powder. Due to its unique physical and chemical properties, silk fibroin materials are currently It has been widely studied in the field of biomedical materials, such as immobilized enzyme materials, cell culture substrates, drug release agents, artificial organs, etc. In order to improve the performance of silk fibroin and make it better used in the field of biomaterials, in recent years, schol...

Examples

Embodiment 1

[0019] 1) Dissolve a certain amount of nano-ZnO in 20 ml of PVP-containing aqueous solution to make the molar ratio 1:1, and react for 4 hours under ultrasonic conditions. After the reaction, the solution is centrifuged, washed, and dried to obtain surface-modified nano ZnO.

[0020] 2) Put silkworm cocoons into a sodium carbonate solution with a mass percentage concentration of 0.5% and stir, boil for 60 minutes to remove sericin on the surface of silkworm cocoons, wash with water, and dry at 60 °C to prepare silk fibroin fibers. The dried silk fibroin The mixed solution of fiber and calcium chloride, water and ethanol with a molar ratio of 1:8:2 is dissolved at 75 °C to form a silk fibroin solution, and the ethanol and calcium chloride in the solution are removed by dialysis, and silk is obtained by filtration. fibroin solution, and then freeze-dried to obtain silk fibroin.

[0021] 3) According to the weight ratio of 1:0.1:0.01, water, silk fibroin, and PVP surface-modifie...

Embodiment 2

[0025] 1) A certain amount of nano-TiO 2 , dissolved in 20 ml of aqueous solution containing Triton X-100 so that the molar ratio was 1:5, and reacted for 4 hours under ultrasonic conditions. After the reaction, the solution was centrifuged, washed, and dried to obtain surface-modified nano-TiO 2 .

[0026] 2) Put silkworm cocoons into a sodium carbonate solution with a mass percentage concentration of 0.5% and stir, boil for 60 minutes to remove sericin on the surface of silkworm cocoons, wash with water, and dry at 60 °C to prepare silk fibroin fibers. The dried silk fibroin The mixed solution of fiber and calcium chloride, water and ethanol with a molar ratio of 1:8:2 is dissolved at 75 °C to form a silk fibroin solution, and the ethanol and calcium chloride in the solution are removed by dialysis, and silk is obtained by filtration. fibroin solution, and then freeze-dried to obtain silk fibroin.

[0027] 3) Nano-TiO surface modified with water, silk fibroin and Triton X...

Embodiment 3

[0030] 1) Dissolve a certain amount of nano-ZnO in 20 ml of PVA-containing aqueous solution to make the molar ratio 1:0.5, and react for 4 hours under ultrasonic conditions. After the reaction, the solution is centrifuged, washed, and dried to obtain surface-modified nano ZnO.

[0031] 2) Put silkworm cocoons into a sodium carbonate solution with a mass percentage concentration of 0.5% and stir, boil for 60 minutes to remove sericin on the surface of silkworm cocoons, wash with water, and dry at 60 °C to prepare silk fibroin fibers. The dried silk fibroin The mixed solution of fiber and calcium chloride, water and ethanol with a molar ratio of 1:8:2 is dissolved at 75 °C to form a silk fibroin solution, and the ethanol and calcium chloride in the solution are removed by dialysis, and silk is obtained by filtration. fibroin solution, and then freeze-dried to obtain silk fibroin.

[0032]3) Hexafluoroisopropanol, silk fibroin and PVA surface-modified nano-ZnO were mixed accordi...

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