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Extraction method of aquatic collagen

An aquatic collagen and extraction method technology, applied in the field of peptide or protein preparation, can solve the problems of poor color, fishy smell, low purity, etc., and achieve the effects of short cycle, good solubility and high purity

Active Publication Date: 2014-02-26
NINGBO XINUOYA MARINE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the disadvantages of fishy smell, low purity and poor color of the products produced by the existing aquatic collagen extraction method, and provides an extraction method of aquatic collagen whose purity, color, smell and other indicators are better than those of the previous products

Method used

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  • Extraction method of aquatic collagen

Examples

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Effect test

Embodiment 1

[0023] A method for extracting aquatic collagen, comprising the steps of:

[0024] a. Raw material processing: raw material cleaning, pulverization, adding 10% NaCl solution 5 times the volume of the raw material, soaking for 5 hours, centrifuging to collect the precipitate, washing the precipitate, sterilizing with damp heat at 115°C for 30 min, and obtaining the crude collagen fiber.

[0025] b. Enzymolysis treatment: adjust the crude collagen fibers to a pH of 8 with food-grade NaOH, prepare a substrate solution with a mass concentration of 50g / L, add 50PPm of compound enzyme, and perform an enzymolysis reaction at 45°C for 8 hours to obtain an enzymolysis solution liquid. The complex enzymes include cellulase, α-galactosidase, mannanase, protease and lipase.

[0026] c. Purification treatment: Enzymolysis solution was inactivated at 70°C for 15 minutes, activated carbon decolorized, the amount of activated carbon added was 3% of the raw material mass, filtered and freeze-...

Embodiment 2

[0028] A method for extracting aquatic collagen, comprising the steps of:

[0029] a. Raw material treatment: the raw material was cleaned and pulverized, added 7% NaCl solution 8 times the volume of the raw material, soaked for 12 hours, centrifuged to collect the precipitate, and sterilized by damp heat at 121°C for 20 minutes to obtain crude collagen fibers.

[0030] b. Enzymolysis treatment: adjust the crude collagen fibers to a pH of 8 with food-grade NaOH, prepare a substrate solution with a mass concentration of 100g / L, add 80PPm of compound enzyme, and perform an enzymolysis reaction at 48°C for 12 hours to obtain an enzymolysis solution liquid. The complex enzymes include cellulase, α-galactosidase, mannanase, protease and lipase.

[0031] c. Purification treatment: Enzymolysis solution was inactivated at 80°C for 12 minutes, decolorized with activated carbon, the amount of activated carbon added was 5% of the raw material mass, filtered and freeze-dried to obtain aq...

Embodiment 3

[0033] A method for extracting aquatic collagen, comprising the steps of:

[0034] a. Raw material processing: raw material cleaning, crushing, adding 10% NaCl solution 10 times the volume of the raw material, soaking for 8 hours, centrifuging to collect the precipitate, washing the precipitate, and sterilizing with damp heat at 125°C for 15 minutes to obtain crude collagen fibers.

[0035] b. Enzymolysis treatment: adjust the crude collagen fibers to a pH of 9 with food-grade NaOH, prepare a substrate solution with a mass concentration of 150g / L, add 100PPm of compound enzyme, and perform an enzymolysis reaction at 50°C for 10 hours to obtain an enzymolysis solution liquid. The complex enzymes include cellulase, α-galactosidase, mannanase, protease and lipase.

[0036] c. Purification treatment: Enzymolysis solution was inactivated at 85°C for 10 minutes, decolorized with activated carbon, and the amount of activated carbon added was 4% of the raw material mass, filtered and...

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Abstract

The invention relates to a preparation method of a peptide or a protein, and discloses an extraction method of an aquatic collagen. The method comprises the following steps: raw material treatment, enzymolysis and purification treatment. The aquatic collagen is obtained by the following specific steps: washing and crushing the raw materials; soaking by sodium chloride liquor and centrifugalizing the raw materials; carrying out enzymolysis by virtue of a compound enzyme for 8-15 hours at 45-50 DEG C; inactivating, filtering, freezing and drying an enzymatic hydrolysate. The collagen product prepared by the method provided by the invention is low in molecular weight and good in solubleness, and can be used for overcoming the technical problems of peculiar smell and fishy smell of conventional aquatic collagens, low purity, poor color and luster and the like. The collagen product is high in purity, uniform in color and luster, free from peculiar smell, simple and convenient in production and shorter in period, and can be widely applied to industries such as foods, health-care products and cosmetics.

Description

technical field [0001] The invention relates to a preparation method of peptide or protein, in particular to an extraction method of aquatic collagen. Background technique [0002] Collagen is an important functional protein with strong biological activity and biological function. It has been widely used in medicine, food, daily chemical industry, biosynthesis and other industrial fields, such as medicinal capsules, surgical materials, food additives , cosmetics, etc. At present, the applied collagen mostly comes from the collagen tissue extracts of mammals or poultry animals such as cattle, pigs and chickens. In recent years, livestock and poultry diseases such as mad cow disease in cattle, foot-and-mouth disease in pigs, and avian influenza in poultry have occurred one after another. The safety of collagen in livestock and poultry has been questioned, and some consumers are allergic to collagen in livestock Propensity. Therefore, the use of collagen extracted from terre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/04
Inventor 诸辉贾艳吴吕平石亚妮
Owner NINGBO XINUOYA MARINE BIOTECH CO LTD
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