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Method for rapid detection of aflatoxin content in grain and food

A kind of aflatoxin, rapid technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long detection time and high detection cost, and achieve the effect of short detection time, low detection cost and high industrial application value

Inactive Publication Date: 2016-02-17
张淑金
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, most of the current inspection and detection methods require special instruments and complicated procedures [4, 5], which have disadvantages such as long detection time and high detection cost.

Method used

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  • Method for rapid detection of aflatoxin content in grain and food
  • Method for rapid detection of aflatoxin content in grain and food
  • Method for rapid detection of aflatoxin content in grain and food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The mensuration of the aflatoxin content of growth in the embodiment 1 peanut

[0036] (1) Take 1 moldy peanut, weigh it, 0.45g, cut it into small pieces, place it in a test tube, add 800 μl of chloroform, stir and extract for 5 minutes, take the extract, and obtain the test product;

[0037] (2) The test sample and the aflatoxin standard sample of step (1) are added to the same thin-layer silica gel plate respectively, and when the test sample and the aflatoxin standard sample are added in the spot, the points are all increased by the point addition amount. The order is added sequentially, and the amount of the test sample and aflatoxin standard sample is shown in Table 1.

[0038] Table 1. The sample volume of the test product and aflatoxin standard sample on the thin-layer silica gel plate

[0039]

[0040]

[0041] The aflatoxin standard sample in Table 1 is a mixture of aflatoxin B1, aflatoxin B2, aflatoxin G1, and aflatoxin G2 in a mass ratio of 1:1:1:1. I...

Embodiment 2

[0053] Detect the types and contents of aflatoxins from Aspergillus parasitica and Aspergillus flavus growing on the culture medium and contaminated peanut samples.

[0054] Samples to be tested: Aspergillus parasitica (Y1) grown on the culture medium, Aspergillus flavus grown on the culture medium (Y2), peanut infected by Aspergillus parasitica (Y3), peanut infected by Aspergillus flavus (Y4).

[0055] Said Y1 and Y2 are the mycelia obtained after inoculating Aspergillus parasitica and Aspergillus flavus respectively on the PDA medium and culturing them at 30°C for 5 days, as shown in figure 2 , it can be seen that Aspergillus parasitica is covered with hyphae on the medium and produces spores (Y1).

[0056] (1) Use a knife to cut out 1cmx1cm agar with mold (0.5cmthick) from Y1 and Y2 respectively, the total amount is 0.5cm 3 . Cut into small pieces, place them in test tubes respectively, add 800μl chloroform, stir and extract for 5 minutes, take the extract, and obtain Y1...

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Abstract

The invention discloses a method for quickly detecting the content of aflatoxin in cereals and foods. The method specifically comprises the steps of crushing or cutting a sample to be detected into small pieces, and then extracting the sample by using an extraction solvent to obtain a test product; dropwise adding the test product and an aflatoxin standard sample on a same thin-layer silica gel plate or filter paper respectively, and comparing the fluorescence colors and fluorescence intensities of the test product and the aflatoxin standard sample under an ultraviolet lamp to judge the content and types of aflatoxin in the test product. The detection method disclosed by the invention is simple in step and short in detection time, can be suitable for detecting a large amount of samples at the same time, and is low in detection cost, very few in solvent amount, environment-friendly and high in industrial application value.

Description

technical field [0001] The invention relates to a method for rapidly detecting aflatoxin in grains and food, and belongs to the technical field of food safety inspection. Background technique [0002] Aflatoxin is a type of mycotoxin. Structurally, aflatoxins are a class of small molecular compounds with low molecular weight synthesized from amino acids, shikimic acid, and malonyl-COA through a special pathway. They can accumulate in fungal hyphae or secreted into the surrounding environment. These toxins are basically produced in agricultural products food by contamination with Aspergillus flavus and Aspergillus parasitica. Aspergillus parasiticus produces four major aflatoxins: B1, B2, G1 and G2, whereas A. flavus produces only two aflatoxins, B1 and B2. Aflatoxin was originally identified and isolated from Aspergillus flavus. Type B aflatoxin shows blue (Blue) fluorescence under ultraviolet light, while type G aflatoxin shows green (Green) fluorescence under ultraviol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90
Inventor 张淑金孙家琪
Owner 张淑金
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