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Artificial feeding method of fragrant pear euzophera pyriella

A technology for the superior spot borer and the superior spot borer pupa, which is applied in the direction of animal husbandry and the like, and achieves the effects of consistent development, neat insect age and simple operation.

Inactive Publication Date: 2015-03-04
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Up to now, there is no relevant report on the method of artificial rearing of C.

Method used

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  • Artificial feeding method of fragrant pear euzophera pyriella
  • Artificial feeding method of fragrant pear euzophera pyriella
  • Artificial feeding method of fragrant pear euzophera pyriella

Examples

Experimental program
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Effect test

Embodiment 1

[0045] The artificial rearing method of embodiment 1 fragrant pear leaf borer

[0046] (1) screening the bacterial strain of pear tree rot pathogen

[0047]The samples of fragrant pear disease were mainly collected from Aksu and Bayingolengzhou in Xinjiang. Indoor separation and purification, observed and recorded the colony characteristics of 60 pear rot pathogen strains on PDA. The 60 strains of P. pear rot were classified into 3 groups. Among them: group Ⅰ (representing strain XJxl5) gray-green easy sporulation group; 32 (53%), group II (representing strain XJxl9 (CGMCC No.8507) yellow easy sporulation group; 25 (42%), group III (representative strain XJxl4), gray-white dystocia group, and its pathogenicity was determined.

[0048] Table 1 Results of analysis of variance of lesion expansion area of ​​different strains

[0049]

[0050]

[0051] Note: 1. Mean values ​​are listed as mean ± standard error; 2. Different letters in the same column indicate extremely si...

Embodiment 2

[0058] The artificial rearing method of embodiment 2 Fragrant pear leaf borer

[0059] The 3 types of bacterial strains screened in Example 1 are prepared into mushroom cakes according to the method of the present invention for subsequent use.

[0060] Mature larvae of Eustoma chinensis were collected in the fragrant pear orchard of the Forestry Institute Extension Station in Aksu Prefecture, and pupated and emerged indoors. Referring to the method of Example 1, 502 newly hatched larvae were obtained, which were respectively placed in petri dishes of three types of bacterial strains. The petri dish was moved to an artificial climate box with a temperature range of 22° C. and a humidity of 60% RH.

[0061] Table 3 Survival rate of Ceratocystis spp. under the conditions of different types of strains

[0062]

[0063] Table 4 Oviposition and hatching rate of females in different population types

[0064]

Embodiment 3

[0065] The artificial rearing method of embodiment 3 Fragrant pear leaf borer

[0066] The type II bacterial strain screened in Example 1 was prepared into mushroom cake according to the method of the present invention for subsequent use. Mature larvae of Eustoma chinensis were collected in the fragrant pear orchard of the Forestry Institute Extension Station in Aksu Prefecture, and pupated and emerged indoors. With reference to the method of Example 1, 750 newly hatched larvae were obtained and placed in a petri dish of pear tree rot fungus. The petri dish was moved to an artificial climate box with a temperature range of 25° C. and a humidity of 60% RH for raising. The results showed that the survival rate of 3rd instar larvae was 90%, the pupation rate was 82.35%, the adult eclosion rate was 76.19%, and the average egg production per head was 25.48.

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Abstract

The invention provides an artificial feeding method of fragrant pear euzophera pyriella. The method includes screening bacterial strains of pear tree rotting bacteria, and culturing the bacterial strains on a PDA culture medium to be in a bacterial cake state for standby use; enabling pupas of the fragrant pear euzophera pyriella to be transformed into adults through emergence, placing the adults into an insect feeding cage with hydromel according to a female-male ratio of 1:1 for mating, and taking out females after mating and placing the same in a preservation box for oviposition; hatching out larvas after eggs are conventionally disinfected, and connecting the larvas into a culture dish which grows to be a bacterial cake until the larvas are transformed into pupas. The artificial feeding method is simple and convenient, a pear tree rotting bacterium cake is taken as feed of the fragrant pear euzophera pyriella to complete the life story of the fragrant pear euzophera pyriella, and a lot of undamaged eggs and accurate larvas and pupas at all ages can be obtained without damaging the eggs and the larvas. The artificial feeding method has the advantages of short developmental duration, high survival rate and the like, and experimental population is provided for in-depth and systematic study of the fragrant pear euzophera pyriella in the field of biology, ecology, prevention and control technology and the like.

Description

technical field [0001] The invention relates to a method for artificially rearing the pear moth. Background technique [0002] Euzophera pyriella Yang belongs to Lepidoptera Euzophera pyriella subfamily Euzophera, named by Yang Jikun in 1994, distributed in Urumqi, Tacheng, Bole, Yining, Changji, Hami in Xinjiang , Turpan, Korla and Aksu and other places, the main hosts are pears, apples, figs, dates, apricots, almonds, peaches and poplars. Related reports indicated that the main distribution areas of pears, apples and jujubes in my country are the potential suitable habitats for C. [0003] Korla Fragrant Pear (Pyrus sinkiangensis Yü), a pear species unique to Xinjiang, exported more than 10,000 tons in 2008, with a value of more than 17 million US dollars. In recent years, due to the continuous expansion of cultivation area, improper supporting management measures, and the rapid increase in the number of older pear trees, Korla fragrant pears are facing serious threats f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
Inventor 温俊宝侯世星吴芳马姝岑刘红霞
Owner BEIJING FORESTRY UNIVERSITY
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