A method for preparing liposomes suitable for water-soluble anthraquinone drugs
A water-soluble lipid and water-soluble technology, applied in the field of liposome preparation for highly efficient loading of water-soluble anthraquinone drugs, can solve the problems of easy aggregation of liposome particle size, low drug encapsulation efficiency, and high cost
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Embodiment 1
[0025] 1. Disperse 2kg of fructose carrier through micronization in the ethanol that has dissolved 400g soybean lecithin, carry out organic solvent recovery process under reduced pressure, liposome membrane material will be adsorbed on the micronization water-soluble support carrier, A finely powdered phospholipid-carrier complex is obtained.
[0026] 2. Using 0.01mol phosphate buffer as a hydration medium, the aforementioned phospholipid-carrier complex is hydrated at 40° C. and 0.8 MPa to obtain liquid multilamellar liposomes. This multilayered liposome is subjected to 400w probe ultrasonic treatment, and the liposome solution turns into a translucent milky white liquid.
[0027] 3. Stir and mix with ammonium sulfate solution as the ionic medium with 188 g of mitoxantrone and the aforementioned liposome solution at a temperature of 55° C., and the drug enters the inner water phase of the liposome from the outer water phase and becomes stable. Clear drug-loaded liposomes for...
Embodiment 2
[0030] 1. Get the mitoxantrone 188g that has been processed through micronization as carrier and be dispersed in the ethanol that has dissolved 400g soybean lecithin, carry out organic solvent recycling under reduced pressure condition, liposome membrane material will be adsorbed on micronization water-soluble On the supporting carrier, the phospholipid-mitoxantrone complex in the form of fine powder is obtained.
[0031] 2. Using 0.01M phosphate buffer as a hydration medium, the aforementioned phospholipid-mitoxantrone complex was hydrated at 55° C. and 1 MPa to obtain liquid multilamellar liposomes. This multilayered liposome is subjected to 400w probe ultrasonic treatment, and the liposome solution turns into a blue band opalescent liquid.
[0032] 3. After obtaining the liposome solution, add a lyoprotectant to carry out vacuum freeze-drying treatment on the liposome at -30°C. Obtain dry liposome powder with good resolubility.
Embodiment 3
[0034] 1. Mix 1230g of micronized glucose carrier and 400g of soybean lecithin with a small amount of ethanol (20mL) and place it in a supercritical incubation tank, and introduce CO under the condition of 13MPa 2 Carry out supercritical incubation for 27 minutes, and quickly evacuate CO 2 , the liposome membrane material will be adsorbed on the micronized water-soluble support carrier to obtain a micropowdered phospholipid-carrier complex.
[0035] 2. Using 0.01mol phosphate buffer as a hydration medium, the aforementioned phospholipid-carrier complex is hydrated at 40° C. and 0.8 MPa to obtain a liquid translucent milky white liquid.
[0036] 3. The sodium chloride solution with a concentration of 1M is used as an ionic medium at a temperature of 37° C. to stir and mix with 177 g of mitoxantrone and the aforementioned liposome solution, and the medicine enters the inner water phase of the liposome from the external water phase and becomes stable. Clear drug-loaded liposomes...
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Abstract
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