Active site of oldenlandia diffusa as well as preparation method and application thereof
A technology of Hedyotis diffusa and its active parts, which is applied in the field of active parts of Hedyotis diffusa and its preparation, can solve the problem that the material basis of hepatitis B is not clear enough, and achieve the effects of less impurities, cost reduction and high purity
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Embodiment 1
[0025] Take 1 kg of Hedyotis diffusa, crush it into coarse powder, add 20 times the amount of 95% ethanol for the first time, reflux extraction for 2 hours, filter to obtain extract I; add 18 times the amount of 95% ethanol for the second time, and reflux extraction for 1.5 hours , filtered to obtain extract II; add 15 times the amount of 95% ethanol for the third time, reflux extraction for 1 hour, filter to obtain extract III; combine the three extracts, recover ethanol under reduced pressure until there is no alcohol smell, and obtain the extract stream extract Dissolve the extract in 1L of hot water at 70-80°C, filter, pass through a D101 macroporous resin column, elute with water, 10% ethanol, and 50% ethanol in sequence, collect the 50% ethanol eluate, and recover the ethanol under reduced pressure to nothing mellow, to obtain the eluate; add 300ml of water to dissolve the eluate, filter, pass through a PA6 polyamide column, and elute with 6 times the column volume of wat...
Embodiment 2
[0028] Take 1 kg of Hedyotis diffusa, crush it into coarse powder, add 20 times the amount of 95% ethanol for the first time, reflux extraction for 2 hours, filter to obtain extract I; add 18 times the amount of 95% ethanol for the second time, and reflux extraction for 1.5 hours , filtered to obtain extract II; add 15 times the amount of 95% ethanol for the third time, reflux extraction for 1 hour, filter to obtain extract III; combine the three extracts, recover ethanol under reduced pressure until there is no alcohol smell, and obtain the extract stream extract Dissolve the extract in 1L of hot water at 70-80°C, filter, pass through an AB-8 macroporous resin column, elute with water, 10% ethanol, and 60% ethanol in sequence, collect the 60% ethanol eluate, and recover ethanol under reduced pressure Until there is no alcohol smell, the eluate is obtained; 300ml of water is added to the eluate to dissolve, filtered, passed through a PA6 polyamide column, and eluted with 6 time...
Embodiment 3
[0031] Take 1 kg of Hedyotis diffusa, crush it into coarse powder, add 20 times the amount of 95% ethanol for the first time, reflux extraction for 2 hours, filter to obtain extract I; add 18 times the amount of 95% ethanol for the second time, and reflux extraction for 1.5 hours , filtered to obtain extract II; add 15 times the amount of 95% ethanol for the third time, reflux extraction for 1 hour, filter to obtain extract III; combine the three extracts, recover ethanol under reduced pressure until there is no alcohol smell, and obtain the extract stream extract Dissolve the extract in 1L of hot water at 70-80°C, filter, pass through an NKA-9 macroporous resin column, elute with water, 10% ethanol, and 30% ethanol in sequence, collect the 30% ethanol eluate, and recover ethanol under reduced pressure Until there is no alcohol smell, the eluate is obtained; 300ml of water is added to the eluate to dissolve, filtered, passed through a PA66 polyamide column, and eluted with 6 ti...
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