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Active site of oldenlandia diffusa as well as preparation method and application thereof

A technology of Hedyotis diffusa and its active parts, which is applied in the field of active parts of Hedyotis diffusa and its preparation, can solve the problem that the material basis of hepatitis B is not clear enough, and achieve the effects of less impurities, cost reduction and high purity

Inactive Publication Date: 2014-03-05
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the material basis for its treatment of hepatitis B has not been clear enough, so far there has been no report on the treatment of hepatitis B with the active part of Hedyotis diffusa

Method used

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  • Active site of oldenlandia diffusa as well as preparation method and application thereof
  • Active site of oldenlandia diffusa as well as preparation method and application thereof
  • Active site of oldenlandia diffusa as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 1 kg of Hedyotis diffusa, crush it into coarse powder, add 20 times the amount of 95% ethanol for the first time, reflux extraction for 2 hours, filter to obtain extract I; add 18 times the amount of 95% ethanol for the second time, and reflux extraction for 1.5 hours , filtered to obtain extract II; add 15 times the amount of 95% ethanol for the third time, reflux extraction for 1 hour, filter to obtain extract III; combine the three extracts, recover ethanol under reduced pressure until there is no alcohol smell, and obtain the extract stream extract Dissolve the extract in 1L of hot water at 70-80°C, filter, pass through a D101 macroporous resin column, elute with water, 10% ethanol, and 50% ethanol in sequence, collect the 50% ethanol eluate, and recover the ethanol under reduced pressure to nothing mellow, to obtain the eluate; add 300ml of water to dissolve the eluate, filter, pass through a PA6 polyamide column, and elute with 6 times the column volume of wat...

Embodiment 2

[0028] Take 1 kg of Hedyotis diffusa, crush it into coarse powder, add 20 times the amount of 95% ethanol for the first time, reflux extraction for 2 hours, filter to obtain extract I; add 18 times the amount of 95% ethanol for the second time, and reflux extraction for 1.5 hours , filtered to obtain extract II; add 15 times the amount of 95% ethanol for the third time, reflux extraction for 1 hour, filter to obtain extract III; combine the three extracts, recover ethanol under reduced pressure until there is no alcohol smell, and obtain the extract stream extract Dissolve the extract in 1L of hot water at 70-80°C, filter, pass through an AB-8 macroporous resin column, elute with water, 10% ethanol, and 60% ethanol in sequence, collect the 60% ethanol eluate, and recover ethanol under reduced pressure Until there is no alcohol smell, the eluate is obtained; 300ml of water is added to the eluate to dissolve, filtered, passed through a PA6 polyamide column, and eluted with 6 time...

Embodiment 3

[0031] Take 1 kg of Hedyotis diffusa, crush it into coarse powder, add 20 times the amount of 95% ethanol for the first time, reflux extraction for 2 hours, filter to obtain extract I; add 18 times the amount of 95% ethanol for the second time, and reflux extraction for 1.5 hours , filtered to obtain extract II; add 15 times the amount of 95% ethanol for the third time, reflux extraction for 1 hour, filter to obtain extract III; combine the three extracts, recover ethanol under reduced pressure until there is no alcohol smell, and obtain the extract stream extract Dissolve the extract in 1L of hot water at 70-80°C, filter, pass through an NKA-9 macroporous resin column, elute with water, 10% ethanol, and 30% ethanol in sequence, collect the 30% ethanol eluate, and recover ethanol under reduced pressure Until there is no alcohol smell, the eluate is obtained; 300ml of water is added to the eluate to dissolve, filtered, passed through a PA66 polyamide column, and eluted with 6 ti...

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Abstract

The invention discloses an active site of oldenlandia diffusa as well as a preparation method and application thereof, belonging to the technical field of traditional Chinese medicines. The active site is prepared by the following steps of: getting the oldenlandia diffusa, adding 95% alcohol 15-20 times the amount of the oldenlandia diffusa, refluxing and extracting, filtering, combining extracting liquor, reducing pressure to recycle a solvent to obtain extract; dissolving the extract by hot water, filtering, carrying out macroporous resin column chromatography, eluting by alcohol liquor, collecting eluant, reducing pressure to recycle the solvent to obtain eluate; dissolving the eluate by water, filtering, carrying out polyamide column chromatography, eluting by water, collecting water eluant, reducing pressure and concentrating, and drying to obtain the active site. The method adopted in the invention is simple and suitable for industrial production; adopted separating materials are cheap and can be recycled, so that cost is lowered; finally, the obtained active site has less impurities, high purity and more obvious pesticide effect.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to an active part of Hedyotis diffusa and its preparation method and application. Background technique [0002] Hedyotis diffusa ( Hedyotis diffusa Willd.) is a Rubiaceae plant, which has the effects of clearing heat and detoxifying, diuretic and swelling, promoting blood circulation and relieving pain. Widely distributed in Guangdong, Guangxi, Hunan, Fujian, Zhejiang and other southern provinces. Oral use is mostly used to treat malignant tumors, hepatitis, appendicitis, urinary system infection, bronchitis, tonsillitis, laryngitis and other diseases, and external use can treat poisonous snake bites, carbuncles, boils and so on. Hedyotis diffusa mainly contains chemical components such as flavonoids, anthraquinones, terpenoids and sterols, and its pharmacological effects include anti-tumor, anti-oxidation, antibacterial, immune regulation and liv...

Claims

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Application Information

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IPC IPC(8): A61K36/748A61P1/16A61P31/20
Inventor 张永勇潘家荣
Owner CHINA JILIANG UNIV
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