Preparation method and application of sodium hydroxide coated capillary hollow micro reactor for methylation of saccharides
A sodium hydroxide, capillary technology, applied in chemical instruments and methods, chemical/physical/physical chemical processes, chemical/physical processes, etc., can solve the problems of complex preparation process, poor permeability, solvent condensation, etc. High base rate, quick reaction, easy to make effect
Inactive Publication Date: 2015-07-22
EAST CHINA UNIV OF SCI & TECH
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Problems solved by technology
However, the preparation process of the sodium hydroxide filled capillary reactor is relatively complicated, and its permeability is poor, and the solvent DMSO used has a high freezing point. In practical applications, it is easy to cause the solvent to condense due to high pressure.
These defects greatly limit its application
Method used
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[0017] 1) Preparation of sodium hydroxide capillary hollow microreactor: Prepare a 1:1 mixed solution of sodium hydroxide saturated solution and methanol, and pass it into a capillary with an inner diameter of 500 μm and a length of 25 cm, so that the mixed solution fills the entire capillary. The capillary was baked in a vacuum oven at 180° C. for 30 minutes to completely evaporate the solvent and crystallize sodium hydroxide on the inner wall of the capillary. Repeat the operation of feeding the mixed solution and drying for 3 times, so that the sodium hydroxide solid coating is fully and evenly coated on the surface of the inner wall of the capillary. Fill the capillary hollow microreactor with anhydrous acetonitrile and fill it with sodium hydroxide, seal both ends with silicon rubber, and store it in a desiccator.
[0018] 2) Permethylation of β-cyclodextrin: 400 μL DMSO was injected into the sodium hydroxide-coated capillary hollow microreactor with a microflow syringe...
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Abstract
The invention relates to a preparation method and application of a sodium hydroxide coated capillary hollow micro reactor for methylation of saccharides. Galactosylated modification of protein is one of the most important, common and complicated protein post-translational modifications in cells, and methylation is the most common derivatization analysis manner for study of saccharides. The capillary hollow micro reactor for methylation of saccharides is designed and manufactured according to the preparation method and the application. The preparation method comprises the steps of mixing a sodium hydroxide saturated solution with methyl alcohol according to certain proportion, and injecting the mixture into a capillary tube, carrying out vacuum drying so as to form a porous sodium hydroxide solid layer on the inner wall of the capillary tube to obtain the sodium hydroxide coated capillary hollow micro reactor. When a reaction liquid containing saccharides flows through the reactor, the reaction is completed in the presence of catalysis of sodium hydroxide. Due to the porous sodium hydroxide structure on the inner wall of the reactor, a large area of alkaline contact environment is offered, and the overall structure of a hollow column guarantees high permeability and stability of the reactor, so that the reactor is applicable to methylation of saccharide samples. The reactor is simple to prepare, is reusable, is convenient to operate in batch, and has the potential of online application.
Description
technical field [0001] The invention relates to a capillary hollow microreactor for methylation of carbohydrates and a preparation method thereof. A solid-phase sugar methylation microreactor with high specific surface area and high permeability is prepared by attaching sodium hydroxide crystals to the inner wall of a capillary to form a surface porous structure. Background technique [0002] Glycosylation of proteins is one of the most important, common and complex protein post-translational modifications in cells. Glycosylation plays an important role in protein folding, transport, and localization [Document 1. Helenius A et al. Science, 2001, 291(5512): 2364-2369], and participates in receptor activation, signal transduction and many other important [Document 2. Ohtsubo K et al. Cell. 2006, 126(5): 855-867], so the study of protein glycosylation is of great significance. Methylation is a common derivatization assay used in carbohydrate research. The currently commonly...
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IPC IPC(8): B01J19/00C08B37/16
Inventor 张维冰高小迪李伟华高方圆张凌怡钱俊红
Owner EAST CHINA UNIV OF SCI & TECH