Bacillus subtilis fermentation medium

A technology of Bacillus subtilis and fermentation medium, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of reducing the activity and quality stability of the bacterial agent, the low rate of spore formation, and the low rate of spores of the bacterial agent. Achieve the effects of promoting good growth, increasing spore production rate, and high spore rate

Inactive Publication Date: 2015-05-20
ZHENGZHOU COLLEGE OF ANIMAL HUSBANDRY ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the current industrial production process, the Bacillus subtilis inoculum has the problems of low spore formation rate and high production cost; the spore rate in the inoculum is low, and bacteria without spores will die in a short time, greatly reducing The activity and quality stability of the bacterial agent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The Bacillus subtilis fermentation medium of the present embodiment consists of the following components in parts by weight: 2 parts of bran, 8 parts of corn flour, 1 part of lactose, 0.5 part of beef extract, 0.2 part of ammonium sulfate, and 0.01 part of sodium hydroxide , 40 parts of astragalus water, 1 part of hawthorn water, and 50 parts of double distilled water. The astragalus water is prepared by the following method: boil the astragalus in water for 30 minutes, then filter, repeat the boiling and filtering twice, and combine the filtrates three times to obtain the product. The hawthorn water is prepared by the following method: boil the hawthorn in water for 30 minutes, then filter, repeat the boiling and filtering twice, and combine the filtrates three times to obtain the product.

[0020] The Bacillus subtilis fermentation medium of this embodiment is prepared by the following method: After mixing astragalus water, hawthorn water and double distilled water in...

Embodiment 2

[0022] The Bacillus subtilis fermentation medium of the present embodiment is composed of the following components in parts by weight: 10 parts of bran, 5 parts of corn flour, 4 parts of lactose, 0.01 part of beef extract, 0.4 part of ammonium sulfate, and 0.3 part of sodium hydroxide , 30 parts of astragalus water, 10 parts of hawthorn water, 62 parts of double distilled water. The astragalus water is prepared by the following method: boil the astragalus in water for 30 minutes, then filter, repeat the boiling and filtering twice, and combine the filtrates three times to obtain the product. The hawthorn water is prepared by the following method: boil the hawthorn in water for 30 minutes, then filter, repeat the boiling and filtering twice, and combine the filtrates three times to obtain the product.

[0023] The Bacillus subtilis fermentation medium of this embodiment is prepared by the following method: After mixing astragalus water, hawthorn water and double distilled water...

Embodiment 3

[0025] The Bacillus subtilis fermentation medium of the present embodiment consists of the following components in parts by weight: 8 parts of bran, 1 part of corn flour, 6 parts of lactose, 0.2 part of beef extract, 0.01 part of ammonium sulfate, and 0.5 part of sodium hydroxide , 20 parts of astragalus water, 6 parts of hawthorn water, and 74 parts of double distilled water. The astragalus water is prepared by the following method: boil the astragalus in water for 30 minutes, then filter, repeat the boiling and filtering twice, and combine the filtrates three times to obtain the product. The hawthorn water is prepared by the following method: boil the hawthorn in water for 30 minutes, then filter, repeat the boiling and filtering twice, and combine the filtrates three times to obtain the product.

[0026] The Bacillus subtilis fermentation medium of this embodiment is prepared by the following method: After mixing astragalus water, hawthorn water and double distilled water i...

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Abstract

A disclosed bacillus subtilis fermentation medium is composed of the following components in parts by weight: 2-10 parts of wheat bran, 1-8 parts of corn flour, 1-6 parts of lactose, 0.01-0.5 part of beef extract, 0.01-0.4 part of ammonium sulfate, 0.01-0.5 part of sodium hydroxide, 20-40 parts of radix astragali aqueous solution, 1-10 parts of haw aqueous solution and 50-74 parts of double-distilled water. According to the bacillus subtilis fermentation medium provided by the inveiton, wheat bran, corn flour, lactose, beef extract, ammonium sulfate, sodium hydroxide, radix astragali aqueous solution and haw aqueous solution are compounded for preparing the fermentation medium, the good growth of bacillus subtilis is improved and the spore generating rate of bacillus subtilis is improved; and also a bacillus subtilis liquid fermented by the medium has the effect of improving immunity and promoting feed intake.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation and relates to a Bacillus subtilis fermentation medium. Background technique [0002] Bacillus subtilis is a kind of Bacillus genus, which belongs to Gram-positive bacteria, without capsule, with perinatal flagella, and can move. As a probiotic, Bacillus subtilis has the following advantages: during the growth of the bacteria, active substances such as subtilisin, polymyxin, nystatin, and gramicidin are produced, and these active substances are effective against pathogenic bacteria or endogenous infections. The conditional pathogenic bacteria have obvious inhibitory effect; can rapidly consume free oxygen in the environment, cause intestinal hypoxia, promote the growth of beneficial anaerobic bacteria, and produce organic acids, reduce the pH value of the intestinal tract, and indirectly inhibit other pathogenic bacteria Growth; stimulate the growth and development of animal immune...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/125
Inventor 边传周乔宏兴王永芬潘春梅
Owner ZHENGZHOU COLLEGE OF ANIMAL HUSBANDRY ENG
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