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Rhodococcus ruber strain and application thereof in treatment of wastewater containing organic pollutants

A technology of organic pollutants, Rhodococcus rubrum, applied in biological water/sewage treatment, water/sludge/sewage treatment, water pollutants, etc. , environmental hazards and other issues, to achieve the effect of protecting health, low production and use costs, and protecting the ecological environment

Active Publication Date: 2014-03-12
ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially nowadays, a lot of industrial wastewater is chemically synthesized wastewater, which has complex components and contains a lot of salt, which increases the difficulty of sewage treatment and makes it difficult for the discharged sewage to meet the standards. The toxic organic pollutants contained in these sewage not only directly affect people and the environment. Hazard, also inhibits the ability of environmental organisms to remove organic pollutants

Method used

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  • Rhodococcus ruber strain and application thereof in treatment of wastewater containing organic pollutants
  • Rhodococcus ruber strain and application thereof in treatment of wastewater containing organic pollutants
  • Rhodococcus ruber strain and application thereof in treatment of wastewater containing organic pollutants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Obtaining and Identification of Example 1 Rhodococcus Erythrococcus ZHY1-6

[0045] 1. Acquisition and identification of strain ZHY1-6

[0046] Take oil-contaminated sludge from Changqing Oilfield, with a salt content of about 3% (mass percentage), take 2g of sludge and add it to basal salt medium, add 5g of crude oil to 100mL of basal salt medium, 37°C, 200 During the cultivation process, observe that the color of the black crude oil in the shake flask gradually becomes lighter until the crude oil is degraded to a minimum, then take 1 mL of the bacterial liquid and inoculate it in the same medium, and repeat the acclimatization and enrichment culture for 3 times.

[0047] Then inoculate 1mL of the enriched bacterial solution to inoculate the cells containing phenol, C 18 In the basal salt medium with alkanes and naphthalene as the only carbon source, the same enrichment culture was carried out for 3 times, and then 1 mL of the bacterial solution was taken for gradient...

Embodiment 2

[0063] Example 2 Degradation of different compounds by bacterial strain ZHY1-6 in high-salt basal liquid medium

[0064] Pick a single colony of strain ZHY1-6 and inoculate it in 100mL liquid medium (10g / L peptone, 5g / L yeast extract powder, 10g / L NaCl, 1g / L glucose, pH7.0-7.2), 37°C, 180 rpm Shake culture for 48 hours per minute to obtain fresh bacterial liquid.

[0065] In 100mL basal salt medium (same as the salt basal medium in Example 1), containing 2% NaCl (g / V), add phenol, 200mg / LC 18 Alkanes, 200mg / L naphthalene, inoculated with 5mL of fresh bacterial solution, 35°C, 180 rpm, cultured on a shaking table for 72 hours, and samples were taken to determine the degradation rate of the compound.

[0066] Table 1 Degradation rate of strain ZHY1-6 to different compounds

[0067] substrate phenol C 18 alkanes naphthalene Degradation rate(%) 91.6 68.5 <55

Embodiment 3

[0068] Example 3 Degradation effect of bacterial strain ZHY1-6 in high-salt phenol-containing pharmaceutical wastewater

[0069] Pick a single colony of strain ZHY1-6 and inoculate it in 100mL liquid medium (10g / L peptone, 5g / L yeast extract powder, 10g / L NaCl, 1g / L glucose, pH7.0-7.2), 37°C, 180 rpm Shake culture for 48 hours per minute to obtain fresh bacterial liquid.

[0070] Take 100mL of high-salt phenol-containing pharmaceutical wastewater with a salt content of about 2%, and detect that the phenol content is 181mg / L.

[0071] Insert 5mL of fresh bacterial solution, place it at 35°C, and culture it on a shaker at 160 rpm. Samples were taken to determine the degradation rate of the compound after 2 days, 3 days, and 4 days of culture.

[0072] Table 2 Removal of phenol by strain ZHY1-6 in high-salt phenol-containing wastewater

[0073] substrate Culture for 2 days 3 days 4 days Degradation rate(%) 61.4 78.6 89.1

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Abstract

The invention discloses a Rhodococcus ruber strain and an application thereof in treatment of wastewater containing organic pollutants. The strain is named as Rhodococcus ruber ZHY1-6, the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.8173. A microbial agent is prepared from the Rhodococcus ruber strain; the Rhodococcus ruber ZHY1-6 and the microbial agent are used in a treatment technology of treating wastewater containing organic pollutants, so as to degrade the organic pollutants in wastewater; when the strain and the microbial agent are used in treatment of high-salt phenol wastewater, hydrocarbon-containing wastewater and perfume wastewater, cost of wastewater treatment is reduced, and the strain and the microbial agent have important significance in protecting ecological environment and people health.

Description

technical field [0001] The invention relates to the technical field of degradation of organic pollutants, in particular to a rhodococcus rubococcus, a microbial agent prepared from the rhodococcus rubococcus and the application of the rhodococcus and the microbial agent in the degradation of organic pollutants. Background technique [0002] Phenols, hydrocarbons and aromatic compounds are important chemical raw materials and the main components of industrial wastewater such as pharmaceuticals and spice wastewater. These compounds are harmful to humans and environmental organisms. Especially nowadays, a lot of industrial wastewater is chemically synthesized wastewater, which has complex components and contains a lot of salt, which increases the difficulty of sewage treatment and makes it difficult for the discharged sewage to meet the standards. The toxic organic pollutants contained in these sewage not only directly affect people and the environment. Harm, but also inhibit t...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/34C02F101/32C02F101/36
Inventor 王慧荣梅荣武韦彦斐李明智张宇
Owner ENVIRONMENTAL SCI RES & DESIGN INST OF ZHEJIANG PROVINCE
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