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Method for extracting resveratrol in polygonum cuspidatum fermentation liquor

A technology for resveratrol and fermentation broth, which is applied in the directions of microorganism-based methods, fermentation, chemical instruments and methods, can solve problems such as high cost and low purity, and achieves low production cost, simple process and little environmental pollution. Effect

Inactive Publication Date: 2014-03-12
HUNAN KEYUAN BIO PRODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction method of resveratrol in Polygonum cuspidatum fermentation broth is generally macroporous resin, silica gel or ion exchange resin single-column chromatography, these traditional techniques are high in cost and low in purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] This embodiment includes the following steps:

[0019] (1) Preparation of endophytic fungus L1 spore suspension: pick pentacyclic bacteria from endophytic fungus L1 PDA slant and place in 10ml of normal saline, fully shake;

[0020] The endophytic fungus L1 is preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC NO.4558 (see the patent application document with the application number 201110130127.1);

[0021] (2) Culture medium preparation: Crush fresh Polygonum cuspidatum to 180 mesh, take 0.45g of 180-mesh fresh Polygonum cuspidatum powder (1.5wt% Polygonum cuspidatum), 0.6g (2%) of absolute ethanol, 0.75g (2.5%) of CMC-Na, NH 4 NO 3 0.06g (0.2%), Na 2 HP0 4 0.018g (0.06%), CaCl 2 0.03g (0.1%) was prepared as a fermentation medium with a pH value of 5.5;

[0022] (3) Fermentation: First, the medium in step (2) was sterilized by moist heat at 121°C for 30 minutes; t...

Embodiment 2

[0028] This embodiment includes the following steps:

[0029] (1) Preparation of endophytic fungus L1 spore suspension: pick pentacyclic bacteria from endophytic fungus L1 PDA slant and place in 10ml of normal saline, fully shake;

[0030] The endophytic fungus L1 is preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC NO.4558 (see the patent application document with the application number 201110130127.1);

[0031] (2) Culture medium preparation: Crush fresh Polygonum cuspidatum to 180 mesh, take 0.45g of 180-mesh fresh Polygonum cuspidatum powder (1.5wt% Polygonum cuspidatum), 0.6g (2%) of absolute ethanol, 0.75g (2.5%) of CMC-Na, NH 4 NO 3 0.06g (0.2%), Na 2 HP0 4 0.018g (0.06%), CaCl 2 0.03g (0.1%) was prepared as a fermentation medium with a pH value of 5.5;

[0032] (3) Fermentation: First, the medium in step (2) was sterilized by moist heat at 121°C for 30 minutes; t...

Embodiment 3

[0038] This embodiment includes the following steps:

[0039] (1) Preparation of endophytic fungus L1 spore suspension: pick pentacyclic bacteria from endophytic fungus L1 PDA slant and place in 10ml of normal saline, fully shake;

[0040] The endophytic fungus L1 is preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC NO.4558 (see the patent application document with the application number 201110130127.1);

[0041] (2) Culture medium preparation: Crush fresh Polygonum cuspidatum to 180 mesh, take 0.45g of 180-mesh fresh Polygonum cuspidatum powder (1.5wt% Polygonum cuspidatum), 0.6g (2%) of absolute ethanol, 0.75g (2.5%) of CMC-Na, NH 4 NO 3 0.06g (0.2%), Na 2 HP0 4 0.018g (0.06%), CaCl 2 0.03g (0.1%) was prepared as a fermentation medium with a pH value of 5.5;

[0042] (3) Fermentation: First, the medium in step (2) was sterilized by moist heat at 121°C for 30 minutes; t...

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Abstract

The invention discloses a method for extracting resveratrol in polygonum cuspidatum fermentation liquor. The method comprises the following steps: (1) preparing an endophytic fungi L1 spore suspension liquid; (2) preparing a culture medium; (3) fermenting so as to obtain the polygonum cuspidatum fermentation liquor; (4) extracting the polygonum cuspidatum fermentation liquor with ethyl acetate for four times, combining ethyl acetate phases, concentrating till drying so as to obtain a crude resveratrol extract; (5) carrying out chromatography jointly through macroporous resin and silica gel; (6) refining so as to obtain the resveratrol product. The method is simple, has low production cost, slightly pollutes the environment, and is applicable to industrial production. The purity of the resveratrol purified and separated from the polygonum cuspidatum fermentation liquor by the method reaches 92wt%.

Description

technical field [0001] The invention relates to a method for extracting resveratrol, in particular to a method for extracting resveratrol in fermented liquid of Polygonum cuspidatum. Background technique [0002] Resveratrol is a plant antibiotic, which mainly exists in grape peels, peanuts, mulberries, pine trees, Polygonaceae (Polygonum cuspidatum) 12 families and 72 species of plants belonging to 31 genera. Resveratrol is a chemical substance produced by organisms to resist environmental stress (such as bad weather, microbial infection, etc.), and has significant pharmacological and biological effects, such as anti-cancer, nerve protection, anti-aging, anti-inflammation, and life extension Wait. [0003] At present, the extraction method of resveratrol in Polygonum cuspidatum fermentation broth is generally macroporous resin, silica gel or ion exchange resin single-column chromatography. These traditional techniques are high in cost and low in purity. It can be seen tha...

Claims

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Application Information

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IPC IPC(8): C12P7/22C07C39/21C07C37/72C07C37/82C12R1/80
Inventor 刘杉林
Owner HUNAN KEYUAN BIO PRODS
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