Method and device for collecting and sampling unconventional components in methanol car tail gas
A technology of automobile exhaust and sampling devices, which is applied in the direction of sampling devices, etc., can solve the problems that samples cannot be used in biological tests and physical and chemical tests, and achieve the effects of easy popularization, simple collection and sampling methods, and moderate instrument prices
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Embodiment 1
[0027] As shown in Figure 1, the device of this embodiment includes a four-way pipe 1, a condensation pipe 2, an absorption pipe 3, a sampling pipe 4, a silica gel drying bottle 5, a gas meter 6, a sampling pump 7 and an ice water tank 8, wherein the four-way pipe The inlet of 1 is connected to the outlet of the automobile exhaust pipe, the condensation pipe 2, the absorption pipe 3 and the sampling pipe 4 are connected in sequence and placed in the ice water tank 8, the inlet of the condensation pipe 2 is connected to the outlet of the four-way pipe 1, the silica gel drying bottle 5, the gas meter 6 is connected to the sampling pump 7 in sequence, and the inlet of the silica gel drying bottle 5 is connected to the outlet of the sampling tube 4 .
[0028] The condensation tube is a self-made glass tube with an outer diameter of 6 mm, a wall thickness of 1.5 mm, and a total length of 2 m; the U-shaped tube is a glass tube with an outer diameter of 11 mm, a wall thickness of 1.5 ...
Embodiment 2
[0034] The conventional components of methanol vehicle exhaust were measured by an automobile exhaust analyzer, and the unconventional components of the above-mentioned collected samples were measured by gas chromatography-mass spectrometry. The measurement results showed that the content of CO in the conventional components was 4.02±0.77%, hydrocarbons The content of compound (HC) is 1870±123ppm, and the content of methanol in unconventional components is 2.85±0.02g / m 3 , the content of formaldehyde is 44.33±0.94mg / m 3 . To sum up, the organic carbon components in methanol vehicle exhaust include methanol, HC, and formaldehyde, and their proportions are 70%, 29%, and 1%. The proportions of these three organic carbon components are 85%, 9%, and 6%. Although the proportions of each component are different, it may be caused by the difference in the vehicle used and the selected working conditions, but the trend is the same. That is, methanol is the most in the tail gas, follow...
Embodiment 3
[0036] The solution sample collected in Example 1 was used as the test substance for the biotoxicity test of methanol automobile exhaust, and its biological effects were studied through cytotoxicity, immunotoxicity and genotoxicity tests. The results showed that: (1) methanol automobile exhaust has an effect on lung cancer The cell line (A549 cells) has an injury effect, can inhibit cell proliferation, and has a certain apoptosis effect on cells; (2) In the immunotoxicity test, methanol automobile exhaust has no effect on the immune activity of lung macrophages, but has an effect on its anti-tumor effect. (3) Among the four genotoxicity tests of methanol automobile exhaust, the cell micronucleus test, the comet test, the Ames test and the TK gene test, the results of the first three genotoxicity tests were negative, while the TK gene test was negative at the highest dose Group 130ml / ml methanol car exhaust showed positive. In addition, the formaldehyde vehicle exhaust has simp...
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