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Detection method for papaya decoction piece

A detection method and technology of decoction pieces, applied in the direction of measuring devices, instruments, scientific instruments, etc.

Inactive Publication Date: 2014-03-19
SHANGHAI JINGFENG PHARMA
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  • Abstract
  • Description
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Problems solved by technology

[0005] The technical problem solved by the present invention is to exist when the oleanolic acid and ursolic acid in the papaya decoction pieces are quantified by existing colorimetric method, spectrophotometry and thin-layer scanning method commonly used for measuring triterpene saponins. Insufficient and provide a kind of detection method of papaya decoction pieces, this detection method has perfected the condition in the content detection method of papaya decoction pieces of Pharmacopoeia, has guaranteed the accuracy of content determination, at the same time, has ensured the required degree of separation, so that inspection work is effective carry out

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  • Detection method for papaya decoction piece
  • Detection method for papaya decoction piece
  • Detection method for papaya decoction piece

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Embodiment Construction

[0013] The present invention intends to proceed from the isolated double bonds in their structures, which will produce ultraviolet absorption at low wavelengths, establishes an HPLC method for the simultaneous determination of oleanolic acid and ursolic acid in papaya, and optimizes the content detection of papaya decoction pieces in the Pharmacopoeia chromatographic conditions.

[0014] For the detection method of papaya decoction pieces of the present invention, the chromatographic conditions during measurement are: chromatographic column: C18 column; mobile phase is methanol-0.1mol / L potassium dihydrogen phosphate solution (90:10); flow rate 1.0mL / min; detection wavelength 210nm; column temperature: room temperature; injection volume 20 μL.

[0015] To be verified by specific examples below:

[0016] 1. Main instruments

[0017] Aileng1100 high-performance liquid chromatography: including high-pressure infusion pump, ultraviolet and visible light detector, column thermost...

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Abstract

The invention discloses a detection method for a papaya decoction piece. The papaya detection piece is detected under the chromatographic conditions of a C18 chromatographic column, flow phase of methanol-0.1mol / L potassium dihydrogen phosphate solution (90:10), flow velocity of 1.0mL / min, detection wavelength of 210nm, column temperature of room temperature and sample size of 20 microliters. According to the detection method, the degree of separation and repeatability of papaya decoction piece detection can be ensured, data analysis and researches on a product are greatly helped, measurement errors are reduced, and the method is particularly suitable for detection work in a laboratory.

Description

Technical field: [0001] The invention relates to the technical field of detection of decoction pieces of traditional Chinese medicine, in particular to a detection method of decoction pieces of papaya. Background technique: [0002] Wild papaya [Chaenom eles cathayensis (Hemsl.) Schneid] is a kind of precious natural plant that integrates medicine and food. It is rich in Zheng'an County, Guizhou Province, which is the "hometown of wild papaya in China". Pentacyclic triterpene saponins such as oleanolic acid and ursolic acid contained in wild papaya are its main physiologically active substances. [0003] Studies in recent years have shown that oleanolic acid and ursolic acid not only have the effects of anti-inflammatory and antibacterial, reducing transaminase, promoting liver cell regeneration, and preventing liver cirrhosis, but also have certain anticancer activities, and ursolic acid is particularly prominent, so It is of great scientific significance to study oleanoli...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 李彤王琼薛亮蔡京
Owner SHANGHAI JINGFENG PHARMA
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