A method for extracting tea polyphenols from tea dregs by compound enzyme method

A compound enzyme method and tea residue technology, which is applied in the direction of drug combination, pharmaceutical formula, medical preparations containing active ingredients, etc., can solve the problems that the safety of tea polyphenols cannot be guaranteed, it is not suitable for large-scale production, and the selection of precipitants is high. problem, achieve the effect of avoiding toxic components, low cost and wide application

Inactive Publication Date: 2016-04-27
BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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Problems solved by technology

[0003] There are many extraction methods of tea polyphenols, but there are the following problems, such as the water extraction method to extract tea polyphenols content is very low; solvent extraction method uses organic solvents in the extraction process, so that toxic substances remain in tea polyphenols, which is not safe The extraction of tea polyphenols by precipitation method has high requirements for the control of pH value. Too high or too low pH is not good for the extraction of tea polyphenols, and the selection of precipitant is high, and some metal salt ions are prone to residue, so that The safety of tea polyphenols cannot be guaranteed; the extraction of tea polyphenols by supercritical carbon dioxide extraction is pollution-free and free of impurities, but the cost is high and it is not suitable for large-scale production; the method of membrane technology to extract tea polyphenols has high cost and low output

Method used

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  • A method for extracting tea polyphenols from tea dregs by compound enzyme method
  • A method for extracting tea polyphenols from tea dregs by compound enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Take tea Camelliasinensis (L.) O. Kuntzes leaf residue 500g, pulverize.

[0028] (2) Add 3 times the amount of distilled water, soak for 1 hour, and add 1.0 g of pectinase.

[0029] (3) Adjust the pH to 5.6 with HCl, enzymatically hydrolyze at 50°C for 2.5 hours, and then boil for 3 minutes to inactivate the enzyme.

[0030] (4) Add ethanol to make the final concentration of ethanol reach 5% by volume, and extract by heating. The tea leaves were extracted twice with ethanol solution with a final concentration of 5% by volume. The extraction conditions are: temperature 77°C, 1 hour each time. Combine the three extracts, filter the extract, centrifuge, take the supernatant, evaporate under reduced pressure, and the extraction amount is 8.332 mg / g.

[0031] (5) Concentrate the supernatant under reduced pressure to obtain the extract, add water to the extract until it is completely dissolved, slowly add the above extract to the AB-8 macroporous adsorption resin, adju...

Embodiment 2

[0034] (1) Take 500g of tealeaves Camelliasinensis (L.) O. Kuntze slag and crush them.

[0035] (2) Add 3 times the amount of distilled water, soak for 1 hour, and add 1.5g of pectinase.

[0036] (3) Adjust the pH to 5.6 with HCl, enzymatically hydrolyze at 50°C for 2.5 hours, and then boil for 3 minutes to inactivate the enzyme.

[0037] (4) Add ethanol to make the final concentration of ethanol reach 5% by volume, and extract by heating. The tea leaves were extracted twice with ethanol solution with a final concentration of 5% by volume. The extraction conditions are: temperature 77°C, 1 hour each time. Combine the extracts for three times, filter the extracts, centrifuge, take the supernatant, evaporate under reduced pressure, and the extraction amount is 7.865mg / g.

[0038] (5) Concentrate the supernatant under reduced pressure to obtain the extract, add water to the extract until it is completely dissolved, slowly add the above extract to AB-8 macroporous adsorption re...

Embodiment 3

[0041] (1) Take 500g of tealeaves Camelliasinensis (L.) O. Kuntze slag and crush them.

[0042] (2) Add 3 times the amount of distilled water, soak for 1 hour, and add 2.0g of pectinase.

[0043] (3) Adjust the pH to 5.6 with HCl, enzymatically hydrolyze at 50°C for 2.5 hours, and then boil for 3 minutes to inactivate the enzyme.

[0044] (4) Add ethanol to make the final concentration of ethanol reach 5% by volume, and extract by heating. The tea leaves were extracted twice with ethanol solution with a final concentration of 5% by volume. The extraction conditions are: temperature 75°C, 1 hour each time. Combine the extracts for three times, filter the extracts, centrifuge, take the supernatant, evaporate under reduced pressure, and the extraction amount is 7.241 mg / g.

[0045] (5) Concentrate the supernatant under reduced pressure to obtain the extract, add water to the extract until it is completely dissolved, slowly add the above extract to LX-60 macroporous adsorption ...

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Abstract

The invention relates to a complex enzyme method for extracting tea polyphenol from tea leaves, which comprises the following specific steps: performing enzymolysis of a tea leaves water solution by a complex enzyme ( pectolase, cellulase and zytase), boiling for 2 min to inactivate the enzyme, wherein the enzymolysis conditions are that the pH is 5.0-6.5, the temperature is 50-60 DEG C and the enzymolysis time is 2-4 hours; extracting the mixture obtained in the last step by a 5-10% ethanol solution through digestion for 3 times, fusing extraction supernatant fluids for concentration, recovering solvent to obtain an extractum, dissolving the extractum in water for adding to macroporous adsorption resin, and adjusting the pH to be 5.0-8.0, wherein the liquid injection amount is 1 / 3 of the column volume; removing impurity by water washing, leaching by a 50-70% ethanol, and recovering tea polyphenol, wherein the dosage of the ethanol is 3-4 times of the resin volume. Compared with a traditional extraction method, the method is high in extraction speed and low in cost, requires no recrystallization, saves time and labor, and is high in extraction rate, low in impurity content, and easy to purify. Moreover, the extraction process requires no poisonous solvent, so that the environmental-friendly purpose is achieved. The project is suitable for large scale industrialized production.

Description

technical field [0001] The invention belongs to the field of natural product or raw material processing, and in particular relates to a method for extracting tea polyphenols from tea dregs by a compound enzyme method. Background technique [0002] Tea dregs are the dregs of tea after brewing. Tea polyphenols (TeaPolyphenols) is the general term for polyphenols in tea, including flavanols, anthocyanins, flavonoids, flavonols and Phenolic acids, etc., the undissolved components are mainly water-insoluble components, such as flavonoids. Tea polyphenols are antioxidants, which have a scavenging effect on free radicals in the human body. Its scavenging effect on free radicals is many times stronger than that of VC and VE. In addition, tea polyphenols and antioxidants also have a synergistic effect. The research on the extraction and separation of tea polyphenols has attracted more and more attention from the world. Discarding tea dregs directly will cause huge waste, so ration...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/82A61P39/06A61P3/06A23L33/00
Inventor 葛喜珍林强田平芳
Owner BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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