A method for isolating prostate cancer PC-3 anticancer peptide and its anticancer peptide by using the heat reactant of crucian carp protein antibacterial peptide liquid

A technology of prostate cancer and PC-3, which is applied to the preparation method of peptides, tetrapeptide components, chemical instruments and methods, etc., can solve the problems of heavy fishy smell, limit the application range of functional food additives, etc., and achieve the improvement of fishy smell Odor, reduce the cost of separation and purification, reduce the effect of processing intensity

Active Publication Date: 2016-04-06
ZHEJIANG OCEAN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the fishy smell of the yellow crucian carp protein antimicrobial peptide solution is relatively heavy, which will limit its application range as a functional food additive

Method used

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  • A method for isolating prostate cancer PC-3 anticancer peptide and its anticancer peptide by using the heat reactant of crucian carp protein antibacterial peptide liquid
  • A method for isolating prostate cancer PC-3 anticancer peptide and its anticancer peptide by using the heat reactant of crucian carp protein antibacterial peptide liquid
  • A method for isolating prostate cancer PC-3 anticancer peptide and its anticancer peptide by using the heat reactant of crucian carp protein antibacterial peptide liquid

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Embodiment Construction

[0035] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0036] 1. Preparation of yellow crucian carp protein antimicrobial peptide solution

[0037] 1) Remove the viscera of the yellow crucian carp, wash and cut into small pieces, and grind it evenly with a tissue grinder to make minced fish. Take 50 grams of minced fish and add 200 grams of deionized water;

[0038] 2) Use 6mol / L hydrochloric acid and 6mol / L sodium hydroxide to adjust the pH value of the mixture of minced fish and water to 2.0 and keep it constant;

[0039] 3) Add 55,000 U of pepsin into the mixture, and perform enzymatic hydrolysis for 2.4 hours while maintaining the enzymatic hydrolysis temperature at 37°C;

[0040] 4) Heat up to 95-100°C to passivate and inactivate the enzyme, and the inactivation time is 10 minutes;

[0041] 5) Cool down to below 4°C and centrifuge for 10 minutes at a speed of 7000r / min;

[0042] 6) Filter,...

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Abstract

The invention relates to a method for separating out a prostatic cancer (PC)-3 anticancer peptide by utilizing a thermal reactant of setipinna taty protein antibacterial peptide liquid and an anticancer peptide obtained by using the method. The method is characterized by comprising the following steps of (1), preparing setipinna taty protein antibacterial peptide liquid, regulating the pH value of the setipinna taty protein antibacterial peptide liquid to 5.0-7.0; (2), thermally treating the setipinna taty protein antibacterial peptide liquid, heating for 30 minutes-60 minutes in a sterilization pot of 100 DEG C-121 DEG C to prepare the thermal reactant of setipinna taty protein antibacterial peptide liquid; and (3), separating out the anticancer peptide from the thermal reactant of the setipinna taty protein antibacterial peptide liquid. Compared with the prior art, the method disclosed by the invention has advantages that setipinna taty protein antibacterial peptide liquid is taken as a starting substrate, and two-step gel separation is carried out, so that the prostatic cancer (PC)-3 anticancer peptide separated from the thermal reactant of the setipinna taty protein antibacterial peptide liquid has purity larger than 94%, is small in molecular weight and is easily absorbed by human body without easily causing anaphylactic reaction. The chemically synthesized peptide of the anticancer peptide shows dose-dependent characteristic for proliferation inhibition of the prostatic cancer (PC)-3 anticancer peptide, and has very obvious apoptosis-inducing effect on PC-3 tumor cells, and can block cell division in a period S.

Description

technical field [0001] The invention relates to an anticancer peptide and a separation method thereof, in particular to a method for separating the prostate cancer PC-3 anticancer peptide and the anticancer peptide by using the heat reactant of the crucian carp protein antibacterial peptide liquid. Background technique [0002] Cancer is currently the most important disease affecting human health. However, most anticancer drugs kill or inhibit cancer cells and at the same time have certain toxic and side effects on the immune system of human health. Anticancer drugs are one of the hot research fields at present. Proteins can release active peptides after moderate enzymatic hydrolysis. In recent years, studies have found that the hydrolyzate of some food-source proteins has good anti-cancer effects, and some anti-cancer peptides have been isolated from them, such as: the molecular weight of 440.9 from anchovy sauce Da hydrophobic anticancer peptide (Lee, Lee, Kim, Kim, & Lee...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/107C07K1/16C07K1/14A61K38/07A61P35/00
Inventor 宋茹韦荣编
Owner ZHEJIANG OCEAN UNIV
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