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Preparation method of synthetic human milk fat substitute (HMFS) of chrysalis oil source

A technology for breast milk fat and silkworm pupa oil, which is applied to milk substitutes, dairy products, applications and other directions, can solve problems such as the undeveloped utilization rate of silkworm pupa oil and the undeveloped market prospect, and achieve the effects of helping growth and development and reducing import pressure.

Active Publication Date: 2014-04-02
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic research on silkworm chrysalis is still at the primary to intermediate stage, the utilization rate of silkworm chrysalis oil has yet to be developed, and the market prospect has yet to be developed (Food Industry, 1997, 5 (5): 42-43.)

Method used

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  • Preparation method of synthetic human milk fat substitute (HMFS) of chrysalis oil source
  • Preparation method of synthetic human milk fat substitute (HMFS) of chrysalis oil source
  • Preparation method of synthetic human milk fat substitute (HMFS) of chrysalis oil source

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] This example illustrates the process of using lipase as a catalyst to catalyze the esterification reaction.

[0031] Refined silkworm chrysalis oil is obtained by physical pressing of silkworm chrysalis. Refined silkworm chrysalis oil was determined by gas chromatography, and it was found that silkworm chrysalis oil contained palmitic acid (C 16:0 ), palmitoleic acid (C 16:1 ), stearic acid (C 18:0 ), oleic acid (C 18:0 ), linoleic acid (C 18：2 ) and linolenic acid (C 18：3 ). After a large number of reaction experiments, it was found that the fatty acid content in each product reached the optimum when the reaction temperature was 65°C, the molar ratio was 1:5, the proportion of reaction enzyme was 5%, and the reaction time was 2 hours.

[0032] After mixing refined silkworm pupa oil (6.5g) and oleic acid (8.46g) in a molar ratio of 1:5, specific lipase Lipozyme RM IM5% (0.748g) was added, and the mixture was placed in a water-bath shaker at 65°C for 0.5h. After the...

Embodiment 2

[0036] This example illustrates the process of using lipase as a catalyst to catalyze the esterification reaction.

[0037] The refined silkworm chrysalis oil (6.5g) was mixed with oleic acid (2.82g) at a molar ratio of 1:1, then specific lipase Lipozyme RM IM3% (0.449g) was added, and the mixture was placed in a water-bath shaker at 65°C for 2 hours. After the reaction, 300 μL was taken out, 2 mL of n-hexane and 2 mL of 0.5 mol KOH-methanol solution were added to react in a water-bath shaker at 65 °C for 1 h, and then detected by gas chromatography, the oleic acid binding rate in silkworm chrysalis oil was measured to be 50.5%. The content of each fatty acid measured in the silkworm chrysalis oil in this example is shown in Table 2 below.

[0038] Table 2 The content of each fatty acid (FA) and the content of Sn-1, 2, and 3 fatty acids measured in silkworm chrysalis oil in Example 2

[0039]

Embodiment 3

[0041] This example illustrates the process of using lipase as a catalyst to catalyze the esterification reaction.

[0042] After mixing refined silkworm pupa oil (6.5g) and oleic acid (8.46g) in a molar ratio of 1:5, specific lipase Lipozyme RM IM5% (0.748g) was added, and placed in a 65°C water-bath shaker for 2h. After the reaction, 300 μL was taken out, and 2 mL of n-hexane and 2 mL of 0.5 mol KOH-methanol solution were added to react in a water-bath shaker at 65 °C for 1 h, and then detected by gas chromatography, the oleic acid binding rate in silkworm chrysalis oil was measured to be 54.4%. The content of each fatty acid measured in the silkworm chrysalis oil in this example is shown in Table 3 below.

[0043] Table 3 The content of each fatty acid (FA) and the content of Sn-1, 2, and 3 fatty acids measured in silkworm chrysalis oil in Example 3

[0044]

[0045] The data measured in this example is the data source of each fatty acid content of silkworm chrysalis oi...

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Abstract

The invention discloses a preparation method of a synthetic human milk fat substitute (HMFS) of a chrysalis oil source. The preparation method is characterized by comprising the steps: mixing chrysalis oil with oleic acid, adding lipase, and performing an oscillating reaction in a shaking bath to obtain the synthetic breast milk fat substitute, wherein the weight ratio of the chrysalis oil to the oleic acid is 1:1-1:9, the lipase accounts for 3-12 percent of weight of a reaction system, the reaction temperature is 25-90 DEG C, and the reaction time is 0.5-10h. At the present stage, most of HMFS used in China are in dependence on import, and the HMFS produced in China can not meet the demand of people; and the preparation method is beneficial to self large-scale production and development in China, and thus the import load is reduced. A large quantity of silkworm chrysalises are idle every year, and the rational utilization of resources is facilitated. Compared with a product prepared from a conventional raw material, the HMFS prepared from the chrysalis oil is rich in polyunsaturated fatty acid linoleic acid and alpha-linolenic acid, accounts for 72.25 percent, accords with the national standard, and is conducive to the growth and development of a baby.

Description

technical field [0001] The invention relates to the field of biocatalysis, in particular to an enzymatic preparation process of a milk fat substitute rich in linolenic acid derived from silkworm chrysalis oil. Background technique [0002] Existing technology: Human milk fat substitute (HMFS), the chemical structure is shown below. [0003] [0004] HMFS chemical structure [0005] Breast milk is the perfect food for a baby's growth and development. WHO recommends breastfeeding babies for at least 6 months after birth to ensure their growth and development and improve their immunity. Breast milk contains about 4-4.5% fat, 98% of which are triglycerides. The types of fatty acids (FA) in breast milk are complex. Saturated fatty acids include medium-chain, medium-long-chain and long-chain saturated fatty acids, such as lauric acid (5-7%), palmitic acid (20-24%), stearic acid (7- 9%); monounsaturated fatty acids include oleic acid (31~38%), palmitoleic acid (2.5~3.8%); n~...

Claims

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Application Information

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IPC IPC(8): A23C11/10
Inventor 赵星宇王俊刘曦王旭东王敏朱蔚杰
Owner JIANGSU UNIV OF SCI & TECH
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