Single-microchannel PCR (Polymerase Chain Reaction) amplification system based on piezoelectric ceramic agitation conveying

Abstract

The invention relates to a single-microchannel PCR (Polymerase Chain Reaction) amplification system based on piezoelectric ceramic agitation conveying. The system comprises a carrier microchannel, power modules and a singlechip control module, wherein the carrier microchannel serves as a biochip; the power of the system is realized by the combined action of piezoelectric ceramics and miniature electromagnetic valves, and the miniature electromagnetic valves are controlled by a singlechip. The system is mainly applied to the subsequent establishment of a real-time fluorescent detection device and an integrated biochip for PCR amplification reaction on a miniature carrier and PCR amplification reaction in a weightless environment, aims to consist of a single-microchannel-cycling PCR amplification reaction module and two kinds of power modules which are respectively applicable to conventional volume and miniature physical check-up, and is suitable for automatically working in a spatial in-orbit weightless environment, so that the aims of functional integration, structure miniaturization, light weight, small size and fully-automatic detection are achieved.

Description

technical field [0001] The invention relates to a single microchannel PCR amplification system, which belongs to the fields of biology, analytical chemistry and medical detection, in particular to a single microchannel PCR amplification system based on piezoelectric ceramic agitation conveying type. Background technique [0002] As manned spaceflight technology matures, its mission cycle will gradually extend, and the requirements for astronauts' health protection will become higher and higher. Unmonitored microorganisms in spacecraft pose a serious threat to the health of astronauts. During long-term spaceflight, the growth of microbial flora and the variation of strains will affect the performance of instruments and equipment and the working environment conditions, and cause harm to the normal operation of spacecraft and its instruments and equipment. In order to prevent the occurrence and spread of infectious diseases in space flight, the microorganisms in the spacecraft...

AI Technical Summary

Problems solved by technology

However, the heating rate and cooling rate in temperature control are not easy to control, and if the temperature is raised too fast, the biological activity in the PCR reagent will be reduced or even the sample bacteria will be lethal.

In addition, the temperature control cycle is very slow, and it cannot drop quickly from the high temperature area to the low temperature area, resulting in the final biological curve not being obvious

Therefore, the temperature control circuit is too large and difficult to be transplanted into the overall biological micro-instrument

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