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A kind of bacillus amyloliquefaciens and microbial bacterial agent and application thereof

A technology of amylolytic spores and microbial agents, applied in the direction of microorganisms, biocides, and methods based on microorganisms, to achieve the effects of increasing photosynthetic area, inhibiting the occurrence of peanut leaf spot, and improving peanut yield

Active Publication Date: 2016-03-23
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the biological control of peanut leaf spot. The purpose of the present invention is to screen out a microbial strain that can efficiently antagonize the pathogenic bacteria of peanut leaf spot, and form a microbial agent to reduce peanut leaf spot by biological control. Harm caused by spot disease to peanut production

Method used

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  • A kind of bacillus amyloliquefaciens and microbial bacterial agent and application thereof
  • A kind of bacillus amyloliquefaciens and microbial bacterial agent and application thereof
  • A kind of bacillus amyloliquefaciens and microbial bacterial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Isolation and Screening of Bacteria with Antagonism to Peanut Leaf Spot

[0041] Soil samples from different ecological environments were collected and bacteria were isolated in the laboratory. After repeated isolation and purification on PB medium, after obtaining pure culture bacteria, Cercospora coccoides was used as the indicator bacteria (such as figure 2 Shown), the strains with high antagonistic performance were screened by the confrontation test method.

[0042] Specific steps: the above-mentioned soil samples were spread in PB medium after gradient dilution, and cultured in a constant temperature incubator at 28°C. The above-mentioned medium was prepared according to the records in the "Microbial Culture Medium Handbook". The formula of the medium was: beef extract 5g, peptone 10g, sodium chloride 5g, agar 15g, tap water 1000ml, pH7.2~7.4, sterilized at 121°C for 20min use. After colonies grow on the plate, single colonies of different shapes were ...

Embodiment 2B

[0044] The bacteriostatic spectrum determination of embodiment 2BA-KA1

[0045] BA- The antibacterial spectrum of KA1, BA-KA1 has a strong antagonistic effect on 9 kinds of pathogenic fungi, and the antagonistic effect on all pathogenic bacteria is more than 40%, among which the inhibitory effect on tomato early blight is the strongest, which is 74.3% .

[0046] Table 1 The inhibitory effect of Bacillus amyloliquefaciens BA-KA1 on different pathogenic fungi

[0047]

Embodiment 3

[0048] Identification of embodiment 3 antagonistic strains

[0049] According to the experimental content and experimental methods recorded in the "Common Bacteria System Identification Manual", the screened preservation number is: CGMCCNo.8341 for identification, and the colony morphology observation is carried out on PB medium. Cultivate at constant temperature for 36 hours to observe the colony morphology. The colony of the strain with the preservation number CGMCCNo.8341 is nearly round, milky white in color, relatively dry and wrinkled on the surface, dull and opaque, eroded on the edge, sunken in the middle, easy to pick, the color of the front and back of the colony or the color of the edge and the center consistent (eg image 3 shown).

[0050] After picking and culturing the colony for 24 hours, after Gram staining, observe under the 100 times oil lens of the optical microscope, as shown in Figure 4 As shown, under a microscope, it can be seen that the strain with...

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Abstract

The invention relates to antagonistic bacteria and microbial preparations for preventing and treating peanut leaf spot, and belongs to the field of biological control of crop diseases. The invention provides a strain with the preservation number of CGMCC? Bacillus amyloliquefaciens BA-KA1 of No.8341 ( Bacillus? amyloliquefaciens ), and the microbial agent containing the bacillus amyloliquefaciens, and the application of the bacillus amyloliquefaciens or the microbial agent in the prevention and treatment of peanut leaf spot is also provided. The Bacillus amyloliquefaciens BA-KA1 with the preservation number of CGMCC No. 8341 and its microbial agent provided by the present invention have significant antagonism against peanut leaf spot, and can prevent the occurrence of peanut leaf spot with a control efficiency of over 70%. The use of the bacterial strain and its microbial preparation can reduce the use of chemical pesticides in the peanut production process, and has good ecological and social benefits.

Description

technical field [0001] The invention relates to a bacillus amyloliquefaciens BA-KA1 strain which has antagonistic effect on peanut leaf spot and a microbial agent capable of preventing and treating peanut leaf spot, belonging to the field of biotechnology. Background technique [0002] Peanut leaf spot is the general term for leaf black spot and brown spot. The disease mainly occurs on the leaves, petioles, stipules, and stems are also affected. Generally, the disease starts on the lower leaves and gradually spreads to the upper leaves. The leaves spread, and when the disease is severe, disease spots can be formed on the stems, petioles, fruit needles, etc. There are clear yellow halos around the leaf spots on the front of the leaves, and the leaves are yellowish brown to dark brown. [0003] At present, farmers do not know enough about peanut leaf spot disease, and think that the leaf spot disease in the later stage of peanut growth basically does not affect the yield, so i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
Inventor 黄亚丽马宏刘昆昂贾振华宋聪赵芊黄媛媛宋水山李书生
Owner BIOLOGY INST OF HEBEI ACAD OF SCI