A method for increasing the content of thermus trehalose synthase

Abstract

The invention relates to a method for increasing the trehalose synthase content of thurmus. The method comprises the following steps: (1) inoculating a liquid seed of a thurmus strain into a liquid culture medium for culturing to prepare a strain cell culture solution; (2) fermenting the strain cell culture solution in a pressure reactor under the pressure to prepare a fermentation solution; (3) centrifuging the fermentation solution, collecting bacterial cells, breaking the cells to prepare a cell breaking solution, and extracting the cell breaking solution by a two-aqueous-phase extraction system to prepare trehalose synthase. According to the method, the trehalose synthase content of the thurmus cells can be increased, the production cost of the trehalose synthase can be reduced, the trehalose synthase can be continuously produced on a large scale, and the production process of the trehalose synthase is simple and convenient.

Description

technical field

[0001] The invention relates to a method for increasing the content of thermus trehalose synthase, which belongs to the technical field of bioengineering. Background technique

[0002] Trehalose synthase (Trehalosesynthase, EC5.4.99.16) is an intramolecular glucosidic transferase that can specifically catalyze the conversion of α-1,4 glycosidic bonds of maltose into α-1,1 glycosidic bonds to generate trehalose. Trehalose is a non-reducing disaccharide formed by the condensation of two glucose molecules through hemiacetal hydroxyl groups. It has the special function of protecting the biofilm and biological macromolecules of organisms under adversity, and can endow lower animals and plants The ability to resist nutrient deficiency, high temperature, high osmotic pressure, dryness, toxic substances and other harsh environments, and microorganisms, so it has broad application prospects in the fields of biological products, food, medicine, crop breeding and fine c...