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On-line detection system for glutamic acid fermentation and online detection method using the system

A technology of glutamic acid and fermentation liquid, which is applied in the field of glutamic acid fermentation, can solve the problems of low reliability and substrate consumption, and achieve the effect of simple structure and good fermentation process

Inactive Publication Date: 2015-12-09
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The object of the present invention is to provide a kind of glutamic acid fermentation on-line detection system and the method adopting this system on-line detection, to solve the time-varying, correlation, hysteresis and unavoidable human intervention of biochemical parameters in the glutamic acid fermentation process and other disturbances, and some less reliable problems in the implementation of mathematical models of substrate consumption, cell growth, and product generation

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  • On-line detection system for glutamic acid fermentation and online detection method using the system
  • On-line detection system for glutamic acid fermentation and online detection method using the system

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Effect test

Embodiment 1

[0063] The bacterial strain transferred in December 2012 is selected as the seed, and the method for online detection of glutamic acid and glucose concentration by the glutamic acid fermentation online detection system comprises the following steps:

[0064] 1) Calibration: the system controls the sampling valve 3 to extract 40uL standard solution from the standard sample pool 4 to the biosensor reaction pool 8, and at the same time, the reaction pool cleaning pump 17 pumps 20mL of buffer solution into the biosensing reaction pool 8 to determine the standard The concentration of glucose and glutamic acid in the solution; two consecutive measurements, the error of the two measurement results is less than 4% (set value), the system shows that the calibration is passed;

[0065] 2) OD value test: set the sampling interval to 7 minutes, the control system controls the sampling pump 14 to extract the fermentation broth in the fermenter to the sampling pool 4 according to the set tim...

Embodiment 2

[0072] The bacterial strain transferred in February 2013 is selected as the seed, and the method for online detection of glutamic acid and glucose concentration by the glutamic acid fermentation online detection system comprises the following steps:

[0073]1) Calibration: the system controls the sampling valve 3 to extract 40uL standard solution from the standard sample pool 4 to the biosensor reaction pool 8, and at the same time, the reaction pool cleaning pump 17 pumps 20mL of buffer solution into the biosensing reaction pool 8 to determine the standard The concentration of glucose and glutamic acid in the solution; three consecutive measurements, the error of the two consecutive measurement results is less than 4% (set value), the system shows that the calibration is passed;

[0074] 2) OD value test: set the sampling interval to 255min, the control system controls the sampling pump 14 to extract the fermentation broth in the fermenter to the sampling pool 4 according to t...

Embodiment 3

[0080] The bacterial strain transferred in June 2013 is selected as the seed, and the method for online detection of glutamic acid and glucose concentration by the glutamic acid fermentation online detection system comprises the following steps:

[0081] 1) Calibration: the system controls the sampling valve 3 to extract 40uL standard solution from the standard sample pool 4 to the biosensor reaction pool 8, and at the same time, the reaction pool cleaning pump 17 pumps 20mL of buffer solution into the biosensing reaction pool 8 to determine the standard The concentration of glucose and glutamic acid in the solution; two consecutive measurements, the error of the two measurement results is less than 4% (set value), the system shows that the calibration is passed;

[0082] 2) OD value test: set the sampling interval to 100min, and the control system controls the sampling pump 14 to extract the fermentation broth in the fermenter to the sampling pool 4 according to the set time i...

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Abstract

The invention discloses an online glutamic acid fermentation detection system and an online detection method using the system, which is used for detecting the concentrations of glucose and glutamic acid on line during glutamic acid fermentation so as to solve the problems of disturbance caused due to time-varying characteristics, relevance and hysteresis of biochemical parameters and inevitable manual interference in a fermentation process of glutamic acid and low reliability in implementing a plurality of mathematical models which are generated with relevance to substrate consumption, bacteria growth and product generation. The online detection method for the concentrations of the glucose and the glutamic acid is finished under automatic control of programs completely; manual sampling is not required; meanwhile, the concentrations of the glucose and the glutamic acid can be simultaneously detected and data can be input to a computer to be saved; the glutamic acid fermentation dynamics is researched according to the concentrations of the glucose and the glutamic acid in different fermentation stages, so that the fermentation process is well controlled.

Description

technical field [0001] The invention relates to the technical field of glutamic acid fermentation, in particular to an online detection system for glutamic acid fermentation and an online detection method using the system. The biomass online detection method in the glutamic acid fermentation process of the present invention can automatically complete the detection of various biological parameters, avoids manual errors and simplifies the measurement process; and can simultaneously detect substrates and target products. Background technique [0002] The purpose of fermentation is to accumulate a large amount of microbial metabolites that people need; the artificial control of metabolism is to artificially break the metabolic control system of microorganisms, so that the metabolism is carried out in the direction that people want. [0003] Biomanufacturing is an advanced production method that uses the biocatalytic function of cells or enzymes for large-scale material processin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N35/00G01N1/28G01N27/26
Inventor 毕春元朱思荣周万里赵晓华史建国
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI