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Endogenous brevibacillus brevis wettable powder

A technology of Bacillus brevis and wettable powder, applied in biocides, bactericides, biocides, etc.

Inactive Publication Date: 2014-04-09
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Bacillus brevis B-011 in this paper has a good antibacterial effect on a variety of plant pathogens, and the prepared endophytic Bacillus brevis B-011 wettable powder has not been reported in the literature so far. Formulation screening of Bacillus wettable powder lays the foundation

Method used

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Experimental program
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Effect test

Embodiment 1

[0015] Embodiment 1 Preparation of Bacillus brevis B-011 fermentation broth

[0016] Seed liquid preparation: pick the B-011 strain preserved on the slant with an inoculation loop, insert it into the BPY liquid medium, the liquid volume is 100ml / 250ml, the initial pH is 6.8, 30°C, shaker at 180r / min and shake for 24h.

[0017] Fermentation culture: Inoculate the seed liquid with 1% (V / V) inoculum amount into 100mL / 250mL fermentation medium, shake and culture at 30°C for 60h, and shaker speed is 150r / min. In the fermented liquid, the spore content is ≥4.0×10 11 , spore rate ≥ 90%.

Embodiment 2

[0019] Take 67kg of endophytic Bacillus brevis B-011 fermentation broth and 11kg of talcum powder to make a mother liquor, dry it with air at 60°C for 80 hours, grind it into powder to make a mother powder, add 8kg of calcium lignosulfonate to the mother powder , 2.85kgDBS, 3kgCMC-Na, 0.15kg dextrin and 8.0kg white carbon black, through spray drying (inlet air temperature 170°C, outlet air temperature 55°C) to obtain the wettable powder of the present invention.

Embodiment 3

[0021] Take 70kg of endophytic Bacillus brevis B-011 fermentation liquid and 10kg of talc powder to make mother liquor, blow dry at 70°C for 76 hours, grind it into powder to make mother powder, add 7kg of calcium lignosulfonate to the mother powder , 3kgDBS, 2kgCMC-Na, 0.1kg dextrin and 7.9kg white carbon black, spray drying (inlet air temperature 172°C, outlet air temperature 58°C) to obtain the wettable powder of the present invention.

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Abstract

The invention relates to endogenous brevibacillus brevis wettable powder which is formed by brevibacillus brevis B-011 (with the collection number of CGMCC No.7466) fermentation liquor, talcum powder, calcium lignosulphonate, DBS (Sodium Dodecyl Benzene Sulfonate), CMC-Na (sodium carboxymethylcellulose), dextrin and white carbon black according to a certain proportion. The prepared brevibacillus brevis B-011 wettable powder achieves the spore content at 1.8*1010 cfu / g at the room temperature of 25 DEG C, the pH value at 6.8, the suspension rate at 76%, the wetting time at 52 seconds and the fineness pass rate at 98%, achieves the survival rate by 90% after being stored at (4+ / -2) DEG C for 100 days, plays prevention and control roles on tomato early blight, can effectively reduce the usage ratio of a chemical pesticide on agricultural production, alleviates the destruction of a pesticide on an ecological environment and meets the requirements of the modern society on the agricultural production and IPM (Integrated Pest Management).

Description

technical field [0001] The invention relates to the field of biological pesticides, in particular to a wettable powder made from endophytic bacteria Bacillus brevis B-011. Background technique [0002] A large number of chemical pesticide preparations are used in our country every year, which can easily cause excessive pesticide residues and bio-enrichment, which not only pollutes the ecological environment, but also affects human health. However, biopesticides have few side effects and are harmless to humans. They are green pesticides with broad prospects, and are increasingly becoming a trend in the development of global pesticides. [0003] Studies in recent years have shown that plant endophytic bacteria have a good inhibitory effect on many plant pathogens, and the research on endophytic bacteria has become a research hotspot in the biological control of plant diseases. Wide range of applications. [0004] The wettable powder not only improves the economic effect, but...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N25/14A01P3/00
Inventor 易有金田健李舒雯
Owner HUNAN AGRICULTURAL UNIV
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