Rhodamine functionalized poly(styrene-alternate galactosyl maleic acid) as well as synthetic method and application thereof
A synthetic method and styrene technology, applied in the field of rhodamine functionalized polymer and its synthesis, can solve the problems that have never been reported, and achieve good auxiliary effect, low background signal, and low toxicity
Inactive Publication Date: 2014-04-09
厦门生光生物科技有限公司
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The use of lectin-saccharide-mediated drug delivery has been reported in the literature, but the use of this mechanism to mediate fluorescent materials for tumor imaging has not been reported
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[0032] 1, the pH titration of macromolecule nanomaterial of the present invention
[0033] Weighing 20 mg of the polymer nanomaterial was dissolved in 2 ml of sterilized water, and ultrasonicated for 1 hour to obtain a 10 mg / ml nanomaterial solution. The solution was diluted 10 times to obtain 1 mg / ml nanomaterial. Pipette 1 mg / ml of nanomaterial 10 μ 1 into 1 ml of disodium hydrogen phosphate-phosphate buffer solution (100 mM) at pH 4-9, and collect fluorescence spectra on a microplate reader modeled as Spectramax M5 (Molecular Device). Use a pipette to pipette 10 μl of 10 mg / ml nanomaterials into 1 ml of disodium hydrogen phosphate-phosphate buffer (100 mM) at different pHs, and collect ultraviolet absorption spectra on a model Spectramax M5 (Molecular Device) microplate reader. figure 1 It is the pH-sensitive ultraviolet absorption spectrogram of the polymer nanomaterial described in the present invention. figure 2 It is the pH-sensitive fluorescence emission spectrum d...
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The invention discloses rhodamine functionalized poly(styrene-alternate galactosyl maleic acid) as well as a synthetic method and application thereof, and relates to poly(styrene-alternate galactosyl maleic acid). The molecular weight of the rhodamine functionalized poly(styrene-alternate galactosyl maleic acid) is 20000, and the rhodamine functionalized poly(styrene-alternate galactosyl maleic acid) is a high molecular nano material. The synthetic method comprises the following steps: adding poly(styrene-alternate maleic acid) into anhydrous N,N-dimethylformamide to obtain high molecular nano material liquor; dissolving rhodamine in the anhydrous N, N-dimethylformamide to obtain rhodamine liquor; adding rhodamine liquor into the high molecular nano material liquor, and then adding triethylamine and galactosamine to obtain a mixture; adding saturated aqueous liquor of sodium hydrogen carbonate into the mixture, dialyzing, drying, dissolving the obtained solid in water and carrying out ultrasonic treatment. The rhodamine functionalized poly(styrene-alternate galactosyl maleic acid) can be applied to preparing tumor imaging agents.
Description
technical field [0001] The invention relates to poly(styrene-alternating galactosylmaleic acid), in particular to rhodamine functionalized poly(styrene-alternating galactosylmaleic acid) and its synthesis method and application. Background technique [0002] With the increasing incidence of cancer worldwide, the development of new cancer treatment modalities has important clinical significance. Surgical resection is widely used in tumor treatment. However, the ability to observe tumor lesions with the naked eye is limited, so it often leads to incomplete tumor removal and thus tumor recurrence. Surgical resection based on fluorescent probes can provide surgeons with the accurate location of tumor lesions, so that cancer tissues can be completely removed (Q.T. Nguyen, R.Y. Tsien, Nat Rev Cancer 2013, 13, 653-662). At present, there are some clinical evidences for the effectiveness of fluorescence-assisted cancer surgery. For example, scientists use fluorescein isothiocyanate...
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IPC IPC(8): A61K49/00C08F212/08C08F222/38C08F8/34
Inventor 韩守法吴选俊
Owner 厦门生光生物科技有限公司