Tissue culture method of anthurium

Abstract

The invention relates to a tissue culture method of anthurium. The method comprises the steps of (1) inducing callus; (2) differentiating adventitious buds; and (3) carrying out subculture proliferating on adventitious buds, and transplanting and nursing. The method is characterized in that when seedlings in step (3) in a regrowth block mass grow to 2cm or more and respectively have 1 to 3 roots, the regrowth block mass can be used as a subsequent transplanting regrowth block mass; the method also comprises a step (4) of transferring the regrowth block mass into a greenhouse to carry out seedling hardening two weeks before the transplanting, and opening a tissue culture bottle cover 3 to 5 days before the transplanting; a step (5) of cutting the large block mass into small block masses, cutting off test-tube seedlings with roots, which is more than 3cm high, and immersing the test-tube seedlings in a chlorothalonil liquid medicine for 30 minutes; step (6) of adopting peat soil or peat soil, perlite and river sand as cultivation substrates, transplanting the regrowth block mass and the test-tube seedlings, wherein the substrate is 1 to 1.5cm away from a seedling sieve notch, the small block mass is used as a transplanting unit, and 2 to 3 test-tube seedlings are used as a transplanting unit; and step (7) of nursing the seedlings after the transplanting. By adopting the method, the environmental applicability of the test-tube seedlings can be improved, and the survival rate can be increased by 8 percent.

Description

Technical field: [0001] The invention relates to plant tissue culture technology, in particular to a method for anthurium tissue culture. Background technique: [0002] Anthurium adndraeanum is a perennial evergreen herb of Araceae Anthurium, which can be used as potted flowers and cut flowers. It is one of the most popular and precious flowers in the world today, with high ornamental value and economic value. [0003] In terms of Anthurium tissue culture research, the predecessors adopted the following steps: [0004] ① Callus induction. In the callus induction culture of Anthurium anthurium, the young leaves after disinfection were inoculated in the culture medium containing the combination of plant growth regulator 2,4-D0.1~1.0mg / L+6-BA1.0~2.0mg / L Dedifferentiation was induced to form callus on modified MS medium; [0005] ② Differentiation of adventitious buds. In the adventitious bud differentiation culture, inoculate the induced callus to the combination containing...

AI Technical Summary

Problems solved by technology

However, the first technological process has disadvantages such as too long technological process, high production cost, and high seedling variation rate, while the second technological process is simplified on the basis of the first technological process, saving the time for strong seedlings of test-tube seedlings. production costs such as labor costs, drug costs, and water and electricity costs in the link and rooting of test-tube seedlings, and can reduce the seedling mutation rate, but in the test-tube seedling domestication and transplanting link, due to the main use of anthurium tissue culture seedlings to separate the regenerated seedlings Cutting into single seedlings for domestication and transplanting and seedling tray cultivation, resulting in high cost of cutting and single seedlings for domestication and transplantation, complicated seedling transplanting management technology and low production efficiency